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pTlpA-Wasabi

Plasmid

#85455

Purpose

mWasabi reporter gene gated by wild type TlpA

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

D341A

Promoter

TlpA and TlpA, LacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pFREE_Amp

Plasmid

#92051

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Article

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Inserts

Cas9
gRNA array

Use

CRISPR

Promoter

pRhamBAD and ptet

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pRS405-FlagPEN2/PS1wt

Plasmid

#207803

Purpose

Yeast integrative vector for expression of FlagPEN2 and Presenilin 1

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Gamma-secretase subunits PEN-2 and presenilin1 wt

Expression

Yeast

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pSiMPls_N + pSiMPls_C

Plasmid

#167203

Purpose

SiMPl plasmid pair (pSiMPls) for use with spectinomycin/streptomycin

Depositor

Barbara Di Ventura

Article

Palanisamy et al ACS Omega. 2021 May 21;6(22):14148-14153

Inserts

EGFP
SmR 1-75 + gp41-1 N-intein
mRuby3
gp41-1 C-intein + SmR 76-263

Expression

Bacterial

Promoter

AmpR, CAT, pBAD, and pTrc

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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TroponinT-GCaMP5-Zeo

Plasmid

#46027

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

GCaMP5-2A-Zeo

Use

Lentiviral

Expression

Mammalian

Promoter

TNNT2

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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HRE-doUnaG

Plasmid

#201713

Purpose

Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a and a PEST sequence

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Expression

Mammalian

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoEGFP-Kan

Plasmid

#44900

Purpose

Yeast tagging vector to create gene fusion with yeast optimized EGFP with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoEGFP

Expression

Yeast

Available Since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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Lyn-tailed mCherry-SEpHluorin

Plasmid

#32002

Depositor

Sergio Grinstein

Article

Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

Insert

Membrane-targeting sequence of Lyn kinase

Expression

Mammalian

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pBbAk-w4-loxP-TT-loxP-mCherry-PCB

Plasmid

#228469

Purpose

Fluorescent reporter for Cre recombinase activity and the PCB production gene. Cre removes a transcription terminator, leading to mCherry production

Depositor

Mary Dunlop

Article

Jafarbeglou et al ACS Synth Biol. 2024 Nov 19. doi: 10.102

Inserts

w4-loxP-TT-loxP-mCherry
Ho1
PcyA

Expression

Bacterial

Promoter

J23106, J23108, and T7A1

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcNDA-NES-PhoCl2c-mCherry

Plasmid

#164037

Purpose

Optogenetic control of protein localization by PhoCl2c

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NES-PhoCl2c-mCherry

Tags

kozak sequence

Expression

Mammalian

Promoter

CMV promotor

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pS448∙CsR

Plasmid

#122096

Purpose

CRISPR/Cas9 counterselection in Gram-negative bacteria

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Inserts

Cas9
sgRNA

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pem7 and Pm

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pETSXM2-pLacI-mtrCAB

Plasmid

#174618

Purpose

Bacterial expression of gene cluster mtrCAB from Shewanella oneidensis MR-1.

Depositor

I-Son Ng

Article

I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

Insert

mtrCAB

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLacI

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGreen_dualluc_ORF_sensor

Plasmid

#55207

Purpose

Dual-luciferase reporter for microRNA targeting in plants

Depositor

Michael Axtell

Article

Liu et al Plant Cell. 2014 Feb;26(2):741-53. doi:

Inserts

Renilla luciferase
Firefly luciferase

Use

Luciferase; Plant transient expression

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pRS402-nicastrin/APH-1a-HA

Plasmid

#207804

Purpose

Yeast integrative vector for expression of Nicastrin and APH-1a-HA

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Gamma-secretase subunits nicastrin and Aph1a

Expression

Yeast

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLX208-cytoFLARE1.0-TEV

Plasmid

#234512

Purpose

Transcriptional reporter for detecting calcium increase in HEK293T (TEVp); the same TEVp involved in FLiCRE2

Depositor

Wenjing Wang

Article

Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

Insert

EGFP-P2A-CaM-V5-TEVp

Use

Lentiviral

Expression

Mammalian

Available Since

July 11, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pHR_Gal4UAS_Tbet_T2A_GFP_PGK_mCherry

Plasmid

#85431

Purpose

Lentiviral vector for Gal4 inducible Tbet T2A GFP and constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human Tbet

Use

Lentiviral

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pME-mTagBFP

Plasmid

#75148

Purpose

TagBFP (blue) fluorophore for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

mTagBFP

Use

Middle entry vector for multisite gateway three f…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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gRNA-MS2x2-TagRFP657

Plasmid

#184441

Purpose

sgRNA with MS2 aptamer, TagRFP657 as selection marker

Depositor

Rhys Allan

Article

Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

Insert

sgRNA-MS2x2, TagRFP657

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pSL1-GFP

Plasmid

#180420

Purpose

Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a GFP promoter.

Depositor

Nancy Moran

Article

Li et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

GFP

Expression

Bacterial

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLX208-cytoFLARE1.0-TF

Plasmid

#234513

Purpose

Transcriptional reporter for detecting calcium increase in HEK293T (transcription factor)

Depositor

Wenjing Wang

Article

Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

Insert

ERT2-MK2-hLOV-TEVcs-NLS-GAL4-FLAG

Use

Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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