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pTF023_His_hsDDA1

Plasmid

#174480

Purpose

Expresses DDA1 in insect cells

Depositor

Eric Fischer

Publication

Faust et al Nat Chem Biol. 2020 Jan;16(1):7-14. doi:

Insert

DDA1 (DDA1 Human)

Tags

His-TEV

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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KG#121

Plasmid

#110879

Purpose

Expresses the C. elegans unc-31 cDNA pan-neuronally

Depositor

Kenneth Miller

Publication

Charlie et al Genetics. 2006 Feb;172(2):943-61. doi: 1

Inserts

rab-3 promoter
unc-31 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Expression

Bacterial

Mutation

Missing first 408bp

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pWJ1

Plasmid

#90484

Purpose

For gene disruption in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

Depositor

Sheng Yang

Publication

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Insert

pIM13 Origin

Expression

Bacterial

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pTXB1-EXO1b D173A

Plasmid

#68269

Purpose

Bacterial expression of human EXO1 D173A (catalytically-dead) mutant

Depositor

Stefano Ferrari

Publication

Eid et al EMBO Rep. 2010 Dec;11(12):962-8. doi: 10

Insert

EXO1 (EXO1 Human)

Tags

Mxe intein/chitin binding domain

Expression

Bacterial

Mutation

catalytically-dead D173A. Please see depositor co…

Promoter

T7

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-TRAIL-R4

Plasmid

#61385

Purpose

Expresses HA tagged TRAIL receptor 4

Depositor

Francis Chan

Publication

Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

Insert

TRAIL receptor 4 (TNFRSF10D Human)

Tags

HA and TNF receptor leader peptide

Expression

Mammalian

Mutation

TRAIL-R4 leader peptide (aa1-29) replaced with TN…

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pN1-CMV-Aff6_V18FdN-IntC-mCherryC-T2A-EGFP (C2)

Plasmid

#210725

Purpose

NIR optogenetic module for conditional protein splicing of split mCherry (C-part)

Depositor

Vladislav Verkhusha

Publication

Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

Insert

C2

Expression

Mammalian

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDuex2-rsFusionRed1

Plasmid

#205758

Purpose

Expression of rsFusionRed1 in mamalian cells

Depositor

Edward Boyden

Publication

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsFursionRed1

Expression

Mammalian

Mutation

wt

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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myr-palm-mCherry2

Plasmid

#197910

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Publication

Petrich et al Biophys J. 2021 Dec 21;120(24):5478-5490

Insert

myristoylated-palmitoylated-mCherry2

Expression

Mammalian

Mutation

none

Available since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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pOxyR_rfp

Plasmid

#194155

Purpose

Red Fluorescent Reporter of intracellular hydrogen peroxide

Depositor

Sven Panke

Publication

Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

Insert

transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein mCherry

Expression

Bacterial

Promoter

PoxyR, PoxyS

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-Stuffer-HepmTurquoise2

Plasmid

#192826

Purpose

Contains stuffer sequence into which sgRNAs can be cloned and expresses mTurquoise2 from hepatocyte-specific promoter

Depositor

Kristin Knouse

Publication

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mTurquoise2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pVITRO1-BFP-NLS

Plasmid

#184458

Purpose

Nucleus-targeted EBFP2 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Publication

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

EBFP2-3xNLS

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YIplac211-ABP1-iGFPx6

Plasmid

#177099

Purpose

Expresses Abp1-iGFPx6 in yeast cells

Depositor

Benjamin Glick

Publication

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

ABP1 (ABP1 Budding Yeast)

Expression

Yeast

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pNIC-CFP

Plasmid

#173074

Purpose

Expression vector for E. coli producing target protein with N-terminal CFP fusion

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Type

Empty backbone

Tags

mCerulean

Expression

Bacterial

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCR8/GW_TOPO_Cdk12_Untagged

Plasmid

#127176

Purpose

Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

Depositor

Phil Sharp

Publication

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)

Use

Entry vector with transgene for gateway cloning

Tags

None

Promoter

None

Available since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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Ubiquitin (1-76; T66V, L67N)

Plasmid

#110755

Purpose

Bacterial expression of human Ubiquitin (1-76; T66V, L67N)

Depositor

David Komander

Publication

Gladkova et al EMBO J. 2017 Dec 15;36(24):3555-3572. do

Insert

UBB (UBB Human)

Expression

Bacterial

Mutation

T66V, L67N

Promoter

T7

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pACYC.Taq.1Amb

Plasmid

#102794

Purpose

Expresses Taq DNA polymerases with a premature stop codon

Depositor

Andrew Ellington

Publication

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Taq DNA polymerase

Expression

Bacterial

Mutation

W168Stop; *(see below)

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Adrb2

Plasmid

#105199

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Publication

Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

Insert

Adrb2 (Adrb2 Mouse)

Use

To ganerate riboprobe

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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HisGB1-Hif1a(776-826)_FIH

Plasmid

#99508

Purpose

Bacterial expression of hydroxylated Hif1a TAD (776-826)

Depositor

Peter Wright

Publication

Sugase et al Protein Expr Purif. 2008 Feb;57(2):108-1

Inserts

Hif1a (776-826) (HIF1A Human)
FIH1 (HIF1AN Human)

Tags

His6GB1

Expression

Bacterial

Promoter

T7

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLacIq-Wasabi

Plasmid

#86112

Purpose

mWasabi reporter gene driven by LacIq promoter

Depositor

Mikhail Shapiro

Publication

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Insert

mWasabi

Tags

6xHis

Expression

Bacterial

Promoter

pLacIq

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-51

Plasmid

#79966

Purpose

Late1-O3-33-myc-PH

Depositor

Neil King

Publication

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-51

Expression

Bacterial and Mammalian

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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