We narrowed to 15,989 results for: LEA

1. pDEST17-ScChd1[Y137A]

   Plasmid

   #221198

   Purpose

   ScChd1-aa118-1274 chromatin remodeler with Y137A mutation

   Depositor

   Gregory Bowman

   Article

   Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

   Insert

   ScChd1 (aa118-1274)
   

   Tags

   6xHis-tag (Precission protease cleavable)

   Expression

   Bacterial

   Mutation

   Y137A

   Promoter

   T7

   Available Since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. MARS-nGFP

   Plasmid

   #205234

   Purpose

   Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

   Depositor

   Jeremy Baskin

   Insert

   PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody (PLEKHA5 Human)
   

   Tags

   HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3-2xMARS-PLD

   Plasmid

   #205237

   Purpose

   Expresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMF

   Depositor

   Jeremy Baskin

   Inserts

   2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
   phospholipase D (PLD)
   

   Tags

   Nuclear Export Sequence and mCherry

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-Hyg-nCas9n

   Plasmid

   #217487

   Purpose

   Expression of nuclear nCas9n

   Depositor

   Bertrand Collet

   Article

   Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21b-hHNF4aORF-1

   Plasmid

   #206959

   Purpose

   The ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the NheI/NotI sites of the pET21b(+), the bacterial expression vector.

   Depositor

   Catharine Ross, Reza Zolfaghari

   Insert

   HNF4a (Hepatocyte Nuclear Factor alpha4 Variant 2) (HNF4A Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac1-bullfrog saxiphilin

   Plasmid

   #140595

   Purpose

   protein expression

   Depositor

   Dan Minor

   Article

   Yen et al Sci Adv. 2019 Jun 19;5(6):eaax2650. doi:

   Insert

   saxiphilin bullfrog
   

   Tags

   GFP-His10

   Expression

   Insect

   Mutation

   Deletion of M20 - please see depositor comment

   Promoter

   polyhedrin promoter

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-NLS-MBP-rATOM(WDR5)

   Plasmid

   #209710

   Purpose

   Mammalian expression of r-ATOM biosensor for WDR5 with nuclear localization signal

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   NLS-MBP-rATOM(WDR5)
   

   Tags

   NLS-MBP

   Expression

   Mammalian

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. MLKS2ec

   Plasmid

   #130855

   Purpose

   Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

   Depositor

   Hank Bass

   Article

   Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

   Insert

   MLKS2
   

   Use

   Gateway entry vector

   Tags

   eGFP-FLAG-HA

   Available Since

   Sept. 28, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. MLKS2exp

   Plasmid

   #130856

   Purpose

   Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

   Depositor

   Hank Bass

   Article

   Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

   Insert

   MLKS2
   

   Tags

   eGFP-FLAG-HA

   Expression

   Plant

   Available Since

   Sept. 28, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pDGE1177

    Plasmid

    #202775

    Purpose

    CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:TREX2-zCas9_tNbEU
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. XLone-GvpB

    Plasmid

    #173798

    Purpose

    Mammalian acoustic reporter gene 1 cassette 1 (expressing GvpB)

    Depositor

    Xiaojun Lian

    Article

    Howells et al Bioeng Transl Med. 2023 Aug 2;9(2):e1058

    Insert

    GvpB
    

    Tags

    mCherry (K187N -please see depositor comments)

    Expression

    Mammalian

    Promoter

    TRE3G promoter

    Available Since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. H5_fold-0_Elsa

    Plasmid

    #201825

    Purpose

    Expresses H5_fold-0_Elsa in bacterial cells

    Depositor

    Nobuyasu Koga

    Article

    Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

    Insert

    H5_fold-0_Elsa
    

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMOD_C2911

    Plasmid

    #161764

    Purpose

    MODULE C plasmid compatible with the Voytas Plant Genome Engineering Tool kit with an Agrobacterium promoter rolD expressing the TREX2 exonuclease.

    Depositor

    Shaun Curtin

    Article

    Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

    Insert

    rolD:TREX2
    

    Expression

    Bacterial

    Promoter

    rolD

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. BPTI-21

    Plasmid

    #195058

    Purpose

    simplyfied amino acid sequence by alanine scanning

    Depositor

    Peter Kim, Yutaka Kuroda

    Article

    Kuroda et al J Mol Biol. 2000 May 5;298(3):493-501. d

    Insert

    bovine pancreatic trypsin inhibitor
    

    Tags

    His tag and trpΔLE leader sequences

    Expression

    Bacterial

    Mutation

    21 amino acids are Alanine out of 58 amino acids

    Promoter

    T7 promotor

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. NAT-TET-OFF – ubiquitin – LEU – 4MYC

    Plasmid

    #193314

    Purpose

    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    Ubiquitin - Leucine - 4MYC N-terminal tagging
    

    Tags

    4MYC

    Expression

    Bacterial

    Available Since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. HYG-TET-OFF – ubiquitin – LEU – 4MYC

    Plasmid

    #193316

    Purpose

    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    Ubiquitin - Leucine - 4MYC N-terminal tagging
    

    Tags

    4MYC

    Expression

    Bacterial

    Available Since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-28a(+)-His(6)-TEV-AtPCO5(wt)

    Plasmid

    #191803

    Purpose

    Full length wt AtPCO5 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO5
    

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-28a(+)-His(6)-AtPCO1(wt)

    Plasmid

    #191798

    Purpose

    Full length wt AtPCO1 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO1
    

    Tags

    6xHis, Thrombin cleavage site

    Expression

    Bacterial

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-28a(+)-His(6)-AtPCO2(wt)

    Plasmid

    #191799

    Purpose

    Full length wt AtPCO2 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO2
    

    Tags

    6xHis, Thrombin cleavage site

    Expression

    Bacterial

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-28a(+)-His(6)-AtPCO3(wt)

    Plasmid

    #191800

    Purpose

    Full length wt AtPCO3 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO3
    

    Tags

    6xHis, Thrombin cleavage site

    Expression

    Bacterial

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge