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Addgene

pSL1419
(Plasmid #219778)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 219778 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDEF
  • Vector type
    Plasmodium, rodent malaria parasites
  • Selectable markers
    Human DHFR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TurboID/GFPmut2
  • Species
    Synthetic
  • Insert Size (bp)
    1709
  • Promoter PbEF1alpha promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid can be integrated into the p230p locus for Plasmodium yoelii (and likely also Plasmodium berghei). The TurboID/GFPmut2 fusion protein is expressed via a PbEF1alpha promoter to create an unfused control protein that is expressed in a cytosolic diffuse manner. This is used as a negative control for TurboID proximity proteomics for proteins that are found in the cytosol.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSL1419 was a gift from Scott Lindner (Addgene plasmid # 219778 ; http://n2t.net/addgene:219778 ; RRID:Addgene_219778)
  • For your References section:

    Global Release of Translational Repression Across Plasmodium's Host-to-Vector Transmission Event. Rios KT, McGee JP, Sebastian A, Moritz RL, Feric M, Absalon S, Swearingen KE, Lindner SE. bioRxiv [Preprint]. 2024 Mar 16:2024.02.01.577866. doi: 10.1101/2024.02.01.577866. 10.1101/2024.02.01.577866 PubMed 38352447