We narrowed to 4,839 results for: pcr
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Plasmid#167348PurposeddPCR gating control for droplets containing either gag+5'pol targets OR 3'pol+tat targetsDepositorInsertpol_gag_tat
UseOtherAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Seattle_IPDA_control_29_006
Plasmid#167352PurposeddPCR gating control for droplets containing either env+5'pol targets OR env+3'pol targetsDepositorInsertpol_env
UseOtherAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Seattle_IPDA_control_32_007
Plasmid#167353PurposeddPCR gating control for droplets containing 5'pol or 3'pol targetsDepositorInsertpol
UseOtherAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Seattle_IPDA_control_17_005
Plasmid#167351PurposeddPCR gating control for droplets containing gat and tat targetsDepositorInsertgat_tat
UseOtherAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pANY1
Plasmid#110949PurposeUniversal mini-vector for efficient cloning of DNA and expression of protein in E. coli.DepositorInsertccd B cassette
TagsHisExpressionBacterialPromoterT7Available sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 9bp dist
Plasmid#234131PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 12bp dist
Plasmid#234130PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 27bp dist
Plasmid#234129PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 15bp dist
Plasmid#234128PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 42bp dist
Plasmid#234127PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 50bp dist
Plasmid#234112PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 46bp dist
Plasmid#234111PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBAD-TRP32
Plasmid#71730Purposeinducible expression of TRP32 in E.coliDepositorInsertTRP32
Tags6xHIS and ThioredoxinExpressionBacterialPromoterpBADAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPL5618_pUG_MtxR
Plasmid#60929PurposePCR template vector for generating methotrexate resistance cassette.DepositorInsertDihydrofolate reductase (DHFR Human)
UseLoxp flanked deletion cassette templateMutationL22F F31SPromoterTEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pattB-aubergine-ADH-gf
Plasmid#69448Purposegenomic fragment of Drosophila aubergine was cloned from w1 background and inserted into pattB plasmid; mutagenesis PCR was used to change the catalytic residue DDH to ADH to express cat-dead AubDepositorInsertaubergine (aub Fly)
TagsNAExpressionInsectMutationchr2L:10,998,626(−)T>GPromoteraubergineAvailable sinceOct. 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMOD4-mT2A-mutated_rpsL-BSD-F
Plasmid#182338PurposeTemplate plasmid for PCR amplification of initial recombineering cassetteDepositorInsertBSD
UseMouse Targeting; Flp / frtExpressionBacterialAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-eGFP-MCS-polyA
Plasmid#174024PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInserteGFP
UseZebrafish knock-in taggingAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPL5617_pUG_PuroR
Plasmid#60928PurposePCR template vector for generating puromycin resistance cassette.DepositorInsertpuromycin N-Acetyltransferase
UseLoxp flanked deletion cassette templatePromoterTEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX20
Plasmid#169443PurposeUsed for guide cloning with PCRDepositorTypeEmpty backboneUseCRISPRAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK782 Septin6-GFP
Plasmid#38296DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSeptin 6 (Septin6 Mouse)
TagseGFPExpressionMammalianMutation22 amino acid linker sequence (added by PCR)PromoterCMVAvailable sinceNov. 21, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by