171,676 results
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Plasmid#208796PurposeXenopus laevis FLAG5-His10-TEV-Cdc45DepositorAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only
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FB407
Plasmid#203628PurposePromoter of the elongation factor 1-alpha gene from P. digitatum (PDIG_59570).DepositorInsertPef1A
UseSynthetic BiologyAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3355
Plasmid#144831PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-MYO10ΔF3
Plasmid#194857PurposeExpress EGFP-MYO10 with the F3 FERM domain lobes deleted (1-1951) in mammalian cells.DepositorInsertMYO10 ΔF3 (MYO10 Human)
TagsEGFPExpressionMammalianMutationTruncated MYO10 construct. MYO10 amino acids 1-1…PromoterCMVAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSin4 UAP1 F383G
Plasmid#169891PurposeUsed for generation of stable mammalian cell lines expressing UAP1 with with F383G mutationDepositorInsertUDP-N-acetylhexosamine pyrophosphorylase (UAP1 Human)
UseLentiviralTagsNoneExpressionMammalianMutationChanged Phenylalanine (F) at position 383 to glyc…Available SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax
Plasmid#174820PurposeMammalian expression of SpCas9 PEmax prime editorDepositorInsertPEmax
TagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBR1k-GFPuv
Plasmid#106382PurposeIPTG-inducible trc promoter expressing gfpuv on BBR1 originDepositorInsertGFPuv
ExpressionBacterialPromotertrcAvailable SinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0043-PspCas13b crRNA backbone
Plasmid#103854PurposeFor cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caacDepositorHas ServiceCloning Grade DNAInsertU6-BbsI-BbsI-PspCas13b DR-polyT
UseCRISPRExpressionMammalianAvailable SinceNov. 28, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGP-CMV-jGCaMP8m
Plasmid#162372PurposeMammalian expression of ultrafast protein calcium sensorDepositorInsertjGCaMP8m
Tags6xHisExpressionMammalianMutationA25G F286YPromoterCMVAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-GFP
Plasmid#171123PurposeDoxycycline inducible expression of GFPDepositorInsertEGFP
UseLentiviralExpressionMammalianPromoterTRE3GSAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRE2 EGFP
Plasmid#129435Purposeexpress GFPDepositorInsertGFP
ExpressionMammalianAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
dCas9-VPR_P2A_mCherry
Plasmid#154193PurposeLentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry markerDepositorInsertsp-dCas9-VPR_P2A_mCherry
UseLentiviralExpressionMammalianMutationD10A, H840APromoterEF1asAvailable SinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV1)
Viral Prep#20298-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEYFP (Cre-dependent)Available SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28c-F30-2xdBroccoli
Plasmid#66843PurposeExpresses two dimeric Broccoli aptamers in the F30 scaffold in bacterial cellsDepositorInsertTwo dimeric Broccoli aptamers in the F30 scaffold
ExpressionBacterialPromoterT7Available SinceJuly 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2 blast
Plasmid#98293PurposeVariant of lentiCRISPRv2 that confers blasticidin S resistanceDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralPromoterEF-1aAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-EGFP (AAV PHP.eB)
Viral Prep#50465-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFPAvailable SinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE6c
Plasmid#207853PurposeMammalian expression of PE6c prime editorDepositorInsertPE6c
TagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationTf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…Available SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-GFP
Plasmid#180020PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-EGFP markerDepositorInsertPEmax-P2A-EGFP
TagsP2A-EGFP, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
anxA2-GFP
Plasmid#107196PurposeExpresses human annexin A2-GFP fusion protein for live cell imagingDepositorInsertannexin A2 (ANXA2 Human)
TagsGreen Fluorescent ProteinExpressionMammalianMutationAla residue 65 in human sequence replaced by Glu …PromoterCMVAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-Mito4x-GCaMP6f
Plasmid#127870PurposeFluorescent reporter for mitochondrial matrix Ca2+DepositorInsert4 repeats of COX8 signal peptide plus GCaMP6f
ExpressionMammalianPromoterCMVAvailable SinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only