165,690 results

1. pAAV-Ef1a-DIO mScarlet (AAV5)

   Viral Prep

   #131002-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO mScarlet (#131002). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO mScarlet plasmid DNA. EF1a-driven, Cre-dependent mScarlet expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Promoter

   EF1a

   Tags

   mScarlet (Cre-dependent)

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

2. FL-fibulin-3-V5-lentiviral-GFP

   Plasmid

   #29703

   Depositor

   Mariano Viapiano

   Article

   Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

   Insert

   EFEMP1 (EFEMP1 Human)
   

   Use

   Lentiviral

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pSL3718 (pQCascade_Pse)

   Plasmid

   #200899

   Purpose

   Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   PseCas7, PseCas8, PseCas6, PseTniQ
   

   Use

   Tags

   NLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-mCherry-IRES-Flpo (AAV Retrograde)

   Viral Prep

   #55634-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-mCherry-IRES-Flpo (#55634). In addition to the viral particles, you will also receive purified pAAV-EF1a-mCherry-IRES-Flpo plasmid DNA. Flpo and bicistronic (IRES) mCherry expression under the EF1a promoter. These AAV preparations are suitable purity for injection into animals. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Promoter

   EF1a and Ef1a/IRES

   Tags

   mCherry

   Available Since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pFUGW-FerH-ffLuc2-eGFP

   Plasmid

   #71393

   Purpose

   Lentiviral vector of luciferase-eGFP fusion gene driven by FerH promoter

   Depositor

   Glenn Merlino

   Article

   Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

   Insert

   FerH-ffLuc2-eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   FerH

   Available Since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. GST-RBD

   Plasmid

   #15247

   Depositor

   Martin Schwartz

   Article

   Ren et al EMBO J. 1999 Feb 1. 18(3):578-85.

   Insert

   Rhotekin (Rtkn Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 7-89 (Rho binding domain)

   Promoter

   Available Since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET30a-AlkB-D135S

   Plasmid

   #79051

   Purpose

   Express mutant AlkB protein (D135S) in E. coli BL21(DE3)

   Depositor

   Tao Pan

   Article

   Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

   Insert

   AlkB (alkB E.coli)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   A truncated AlkB with deletion of the amino (N)-t…

   Promoter

   T7

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDicAID_nCas9-PmCDA_NptII_Della

   Plasmid

   #91694

   Purpose

   Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A for nickase Cas9

   Promoter

   PcUbi

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFC333

   Plasmid

   #87844

   Purpose

   AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

   Inserts

   Cas9
   ble (bleomycin resistance marker)
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and Aspergillu…

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-ChroME2f-mRuby2-ST

    Plasmid

    #170160

    Purpose

    Cation channelrhodopsin ChroME2f fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2f-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-FLEX-tdTomato (AAV1)

    Viral Prep

    #28306-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Promoter

    CAG

    Tags

    tdTomato (Cre-dependent)

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

12. pHAGE-mt-mKeima

    Plasmid

    #131626

    Purpose

    lentiviral expression of mitochondrial mKeima for generation of stable cell lines

    Depositor

    Richard Youle

    Article

    Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

    Insert

    mt-mKeima
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-TetOne-Puro-p21

    Plasmid

    #171122

    Purpose

    Doxycycline inducible expression of p21

    Depositor

    Jason Sheltzer

    Article

    Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

    Insert

    p21 (CDKN1A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt (AAV1)

    Viral Prep

    #175181-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt (#175181). In addition to the viral particles, you will also receive purified pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt plasmid DNA. Synapsin-driven, Cre-dependent, third generation glutamate sensor iGluSnFR3, variant 857. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Promoter

    Syn

    Tags

    None

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

15. pHCMV_DENV1_PrME

    Plasmid

    #207263

    Purpose

    Mammalian expression of Dengue virus 1 PrME protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    DENV1_PrME (POLY Dengue virus 1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSL1425 (pSPIN, pCDF backbone)

    Plasmid

    #160735

    Purpose

    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Str-Ii_neomycin

    Plasmid

    #65312

    Purpose

    Cytoplasmic ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Invariant chain (p33)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Streptavidin in its cytoplasmid part

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pdestMB14_sur5p-dCas9-VP64

    Plasmid

    #177788

    Purpose

    Overexpression of dCas9-VP64 in C. elegans

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    dCas9-VP64
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. BL21ΔABCF

    Bacterial Strain

    #102270

    Purpose

    ompA, lamB, ompC, ompF quadruple knockout strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    Tetracycline

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pBabe-puro largeTcDNA

    Plasmid

    #14088

    Depositor

    William Hahn

    Article

    Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

    Insert

    Large T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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