165,586 results

1. pT2KXIG (Tol2)-tp63:ikk1:GFP

   Plasmid

   #105646

   Purpose

   Ikk1(alpha) expression in basal keratinocytes

   Depositor

   Sandra Rieger

   Article

   Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

   Insert

   tp63:ikk1:GFP (chuk Zebrafish)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. IDG_CACNA2D2_OE_1

   Plasmid

   #161616

   Purpose

   Overexpresses CACNA2D2

   Depositor

   Mike McManus

   Insert

   CACNA2D2 (CACNA2D2 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-EF1a-jGCaMP8f-WPRE (AAV1)

   Viral Prep

   #176756-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from AAV-EF1a-jGCaMP8f-WPRE (#176756). In addition to the viral particles, you will also receive purified AAV-EF1a-jGCaMP8f-WPRE plasmid DNA. EF1a-driven expression of calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Loren Looger

   Promoter

   EF1a

   Tags

   None

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

4. TFORF0952

   Plasmid

   #143894

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-UCOE-EF1a-Zim3-dCas9-loxP-P2A-EGFP-loxP

   Plasmid

   #188773

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)_Pmel-1_TCRB

   Plasmid

   #175288

   Purpose

   Expresses Pmel-1 TCR beta chain

   Depositor

   Jeff Hammerbacher

   Article

   Gottschalk et al bioRxiv 2021.11.30.470597

   Insert

   T cell receptor beta chain (Tcrb Mouse)
   

   Use

   Suitable for ivt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)_Pmel-1_TCRA

   Plasmid

   #175287

   Purpose

   Expresses Pmel-1 TCRA alpha chain

   Depositor

   Jeff Hammerbacher

   Article

   Gottschalk et al bioRxiv 2021.11.30.470597

   Insert

   T cell receptor alpha chain (Tcra Mouse)
   

   Use

   Suitable for ivt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pML333_LAMP1-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227898

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   LAMP1 homology arms with mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pML334_RAB7A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227899

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   RAB7A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSynapsin1-axon-jGCaMP8f-P2A-mRuby3 (AAV1)

    Viral Prep

    #172923-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-hSynapsin1-axon-jGCaMP8f-P2A-mRuby3 (#172923). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-jGCaMP8f-P2A-mRuby3 plasmid DNA. hSyn-driven expression of jGCaMP8f in axons with cytosolic expression of mRuby3. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Promoter

    Syn

    Tags

    mRuby3

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

11. pML531_TOMM20-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227890

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    TOMM20 homology arms with mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWZL Neo Myr Flag PLK1

    Plasmid

    #20589

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    PLK1 (PLK1 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. plRL61

    Plasmid

    #163633

    Purpose

    Optimized M. smegmatis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634).

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet-On

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAlbumin

    Plasmid

    #22037

    Depositor

    Didier Trono

    Insert

    partial sequence of human albumine
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRcCMV myc-Plk1 T210D (Nigg LA183)

    Plasmid

    #41158

    Depositor

    Erich Nigg

    Article

    Smits et al Nat Cell Biol. 2000 Sep;2(9):672-6.

    Insert

    Plk1 (PLK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T210D

    Promoter

    CMV

    Available since

    Feb. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pSM.wrmScarlet1-10::SL2::GFP1-10

    Plasmid

    #234318

    Purpose

    For the expression of wrmScarlet1-10 and GFP1-10 in C. elegans

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al Genetics. 2024 Dec 22:iyae214. doi: 10.1

    Insert

    wrmScarlet1-10::SL2::GFP1-10
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCG-TPMV Fd32

    Plasmid

    #163964

    Purpose

    Expression of TPMV F glycoprotein in mammalian cells

    Depositor

    Jakob Reiser

    Article

    Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

    Insert

    TPMV F glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lacking 32 amino acids at C-terminus

    Promoter

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAT203

    Plasmid

    #134880

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLJC6-NADK2-3xFLAG

    Plasmid

    #233895

    Purpose

    lentiviral overexpression vector for NADK2

    Depositor

    Jason Cantor

    Article

    Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

    Insert

    NADK2 (NADK2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGal4BD-CRY2PHR

    Plasmid

    #28244

    Purpose

    Expresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activation

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    cry2 (CRY2 Mustard Weed)
    

    Use

    Tags

    Gal4BD

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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