185,421 results

1. pcDNA3-AKAR4-NES

   Plasmid

   #64727

   Purpose

   Cytosol-targeted, FRET-based PKA activity reporter.

   Depositor

   Jin Zhang

   Article

   Herbst et al Mol Cell Biol. 2011 Oct;31(19):4063-75.

   Insert

   AKAR4-NES
   

   Use

   Tags

   Cerulean, NES, and cpVenus[E172]

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. C1-CFP-Lyn11

   Plasmid

   #155234

   Purpose

   membane marker

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   Lyn11 membrane targeted CFP. Membrane control
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)/Puro/TagRFP-LL5b

   Plasmid

   #112828

   Purpose

   transient/stable overexpression

   Depositor

   Tomasz Prószyński, Joshua Sanes

   Article

   Proszynski et al J Cell Sci. 2013 May 15;126(Pt 10):2225-

   Insert

   LL5b (Phldb2 Mouse)
   

   Use

   Tags

   TagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1.Gα11-LgB97

   Plasmid

   #134361

   Purpose

   Nanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   Gα11-LgB97 (GNA11 Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. AiP11244 - pAAV-hDLXI56i-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1244)

   Plasmid

   #163493

   Purpose

   Direct-expressing SYFP2 AAV Virus

   Depositor

   Boaz Levi, The Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBetaGlobin

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1.Gα13-LgB106

   Plasmid

   #134364

   Purpose

   Nanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   Gα13-LgB106 (GNA13 Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. LaM8-AK74-irFP

   Plasmid

   #159592

   Purpose

   Opto-Nanobody

   Depositor

   Jared Toettcher, Jose Avalos

   Article

   Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

   Insert

   LaM8 with AsLOV2 at AK74
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabepuro-HA-GASC1

   Plasmid

   #24182

   Purpose

   Mammalian expression of human JMJD2C with HA tag

   Depositor

   Kristian Helin

   Article

   Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

   Insert

   GASC1 (KDM4C Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pBabepuro-HA-GASC1

   Plasmid

   #24182

   Purpose

   Mammalian expression of human JMJD2C with HA tag

   Depositor

   Kristian Helin

   Article

   Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

   Insert

   GASC1 (KDM4C Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pEPIP_PepTile_40merFragments_65CancerDrivers

    Pooled Library

    #172109

    Purpose

    Lentiviral peptide expression library for overexpression fitness screening

    Depositor

    Prashant Mali

    Article

    Ford et al Cell Syst. 2021 Jul 21;12(7):716-732.e7.

    Use

    Lentiviral

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

11. pEGFP-C2_SEPT9 i4

    Plasmid

    #71617

    Purpose

    mammalian expression of human SEPT9 i4 with a GFP fusion

    Depositor

    Elias Spiliotis, Cristina Montagna

    Article

    Connolly et al Breast Cancer Res. 2011 Aug 10;13(4):R76

    Insert

    Septin 9 (SEPTIN9 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-ACE2-his expression plasmid

    Plasmid

    #158089

    Purpose

    Expresses ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Lyn-AKAR4

    Plasmid

    #61620

    Purpose

    Lipid raft-targeted FRET biosensor for monitoring PKA activity

    Depositor

    Jin Zhang

    Article

    Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

    Insert

    Lyn-AKAR4
    

    Use

    Tags

    Cerulean, N-terminal targeting sequence from Lyn …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLCS105

    Plasmid

    #209358

    Purpose

    Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal Dendra2 fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221_SLC22A5

    Plasmid

    #132048

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC22A5 (SLC22A5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pUFlip-floxed2A-mRFP-; gcry1:BFP -1

    Plasmid

    #173887

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-mRFP; gcry1: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF0481

    Plasmid

    #142638

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HIF1A (HIF1A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF3518

    Plasmid

    #144994

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NHLH2 (NHLH2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHTC-HaloTag_C9orf72:1-481

    Plasmid

    #210987

    Purpose

    Mammalian expression of full-length human C9orf72. C-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    C9orf72:M1-F481 (C9orf72 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pTol2-10xUAS-Voltron

    Plasmid

    #119039

    Purpose

    UAS-driven expresion of Voltron in zebrafish

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Insert

    Voltron
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    E1b

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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