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pdCas9 Citations (14)

Plasmid Article: Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system.
Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA Nucleic Acids Res. 2013 Jun 12.
PubMed Journal

Articles Citing pdCas9

Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology. Jusiak B, Cleto S, Perez-Pinera P, Lu TK. Trends Biotechnol. 2016 Jan 22. pii: S0167-7799(15)00274-7. doi: 10.1016/j.tibtech.2015.12.014. PubMed
Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, Linhardt RJ, Koffas MA. Nucleic Acids Res. 2016 Apr 13. pii: gkw231. PubMed

Associated Plasmids

Artificial symmetry-breaking for morphogenetic engineering bacterial colonies. Nunez IN, Matute TF, Del Valle I, Kan A, Choksi A, Endy D, Haseloff J, Rudge T, Federici F. ACS Synth Biol. 2016 Oct 30. PubMed
tCRISPRi: tunable and reversible, one-step control of gene expression. Li XT, Jun Y, Erickstad MJ, Brown SD, Parks A, Court DL, Jun S. Sci Rep. 2016 Dec 20;6:39076. doi: 10.1038/srep39076. PubMed

Associated Plasmids

CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production. Cress BF, Leitz QD, Kim DC, Amore TD, Suzuki JY, Linhardt RJ, Koffas MA. Microb Cell Fact. 2017 Jan 17;16(1):10. doi: 10.1186/s12934-016-0623-3. PubMed
Tuning of human MAIT cell activation by commensal bacteria species and MR1-dependent T-cell presentation. Tastan C, Karhan E, Zhou W, Fleming E, Voigt AY, Yao X, Wang L, Horne M, Placek L, Kozhaya L, Oh J, Unutmaz D. Mucosal Immunol. 2018 Nov;11(6):1591-1605. doi: 10.1038/s41385-018-0072-x. Epub 2018 Aug 16. PubMed
Conversion of staphylococcal pathogenicity islands to CRISPR-carrying antibacterial agents that cure infections in mice. Ram G, Ross HF, Novick RP, Rodriguez-Pagan I, Jiang D. Nat Biotechnol. 2018 Nov;36(10):971-976. doi: 10.1038/nbt.4203. Epub 2018 Sep 24. PubMed
Enhancing the Translational Capacity of E. coli by Resolving the Codon Bias. Lipinszki Z, Vernyik V, Farago N, Sari T, Puskas LG, Blattner FR, Posfai G, Gyorfy Z. ACS Synth Biol. 2018 Nov 16;7(11):2656-2664. doi: 10.1021/acssynbio.8b00332. Epub 2018 Nov 2. PubMed
CRISPR-interference-based modulation of mobile genetic elements in bacteria. Nyerges A, Balint B, Cseklye J, Nagy I, Pal C, Feher T. Synth Biol (Oxf). 2019;4(1):ysz008. doi: 10.1093/synbio/ysz008. Epub 2019 Mar 15. PubMed
Increased 3'-Phosphoadenosine-5'-phosphosulfate Levels in Engineered Escherichia coli Cell Lysate Facilitate the In Vitro Synthesis of Chondroitin Sulfate A. Badri A, Williams A, Xia K, Linhardt RJ, Koffas MAG. Biotechnol J. 2019 Jun 10:e1800436. doi: 10.1002/biot.201800436. PubMed
A single plasmid based CRISPR interference in Synechocystis 6803 - A proof of concept. Kirtania P, Hodi B, Mallick I, Vass IZ, Feher T, Vass I, Kos PB. PLoS One. 2019 Nov 26;14(11):e0225375. doi: 10.1371/journal.pone.0225375. eCollection 2019. PubMed
I-Block: a simple Escherichia coli-based assay for studying sequence-specific DNA binding of proteins. Szentes S, Zsibrita N, Koncz M, Zsigmond E, Salamon P, Pletl Z, Kiss A. Nucleic Acids Res. 2020 Mar 18;48(5):e28. doi: 10.1093/nar/gkaa014. PubMed

Associated Plasmids

Regulation of Microbial Metabolic Rates Using CRISPR Interference With Expanded PAM Sequences. Kim B, Kim HJ, Lee SJ. Front Microbiol. 2020 Feb 28;11:282. doi: 10.3389/fmicb.2020.00282. eCollection 2020. PubMed
Lowering DNA binding affinity of SssI DNA methyltransferase does not enhance the specificity of targeted DNA methylation in E. coli. Slaska-Kiss K, Zsibrita N, Koncz M, Albert P, Csabradi A, Szentes S, Kiss A. Sci Rep. 2021 Jul 27;11(1):15226. doi: 10.1038/s41598-021-94528-3. PubMed

If you have published an article using this plasmid, please email us at [email protected] to have your article added to this page.