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pHR_Gal4UAS_Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_PGK_mCherry

Plasmid

#85434

Purpose

Lentiviral vector for Gal4 inducible leucinezipper Pembrolizumab and constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

Pembrolizumab

Use

Lentiviral

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCN-EF2132tet

Plasmid

#107191

Purpose

Expresses EF2132 recombinase for performing recombineering in S. aureus

Depositor

Steve Salipante

Article

Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

Inserts

EF2132
tetR

Expression

Bacterial

Promoter

p23

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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HBV 1.3-mer P-null replicon

Plasmid

#65462

Purpose

containing 1.3 units of the P-null HBV genome (subtype ayw)

Depositor

Wang-Shick Ryu

Article

Wang et al J Virol. 2009 Jun;83(11):5815-24. doi: 1

Insert

1.3 units of the P-null HBV genome

Expression

Mammalian

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pDT305

Plasmid

#128801

Purpose

Human codon optimized dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone

Depositor

Bradley Bernstein

Article

Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

Insert

dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone

Use

CRISPR and Lentiviral

Promoter

hUBC promoter

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-cPLA2-mEGFP

Plasmid

#184718

Purpose

Exogenous cPLA2WT

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

cPLA2-mEGFP

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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p201B Cas9

Plasmid

#59177

Purpose

Cas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
BAR

Use

CRISPR

Promoter

2x35S

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomRuby2-Kan

Plasmid

#44953

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomRuby2

Expression

Yeast

Available Since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoEGFP-CaURA3

Plasmid

#44872

Purpose

Yeast tagging vector to create gene fusion with yeast optimized EGFP with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoEGFP

Expression

Yeast

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pTol2-hspDRv7_scGstlt

Plasmid

#108870

Purpose

scGESTALT V7 lineage barcode for zebrafish

Depositor

Alex Schier

Article

Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

Insert

scGESTALT lineage tracing target V7

Use

Tol2 integration into zebrafish genome

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-4493NES(Booster-PKA)

Plasmid

#184711

Purpose

PKA biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

Booster-PKA

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAureus-TnCAM

Plasmid

#199807

Purpose

vector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureus

Depositor

Steve Salipante

Article

Lo et al Infect Immun. 2023 Oct 17;91(10):e002282

Insert

transposon cassette

Expression

Bacterial

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAbaMCi-BsaI_pJMP2776

Plasmid

#208890

Purpose

Acinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).

Depositor

Jason Peters

Article

Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

Insert

dCas9, sgRNA expression cassette

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Tg (mpx:mCherry-2A-Rac2D57N)

Plasmid

#58934

Purpose

expresses dominant negative rac2 tagged with mcherry in neutrophils

Depositor

Anna Huttenlocher

Article

Deng et al Dev Cell. 2011 Oct 18;21(4):735-45. doi:

Insert

mcherry-2a-Rac2D57N (rac2 Zebrafish)

Tags

mcherry2a

Mutation

changed Aspartate 57 to Asparagine

Promoter

mpx

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pHnCBS1D

Plasmid

#52065

Purpose

expresses nine genes from the H. neapolitanus carboxysome operon and CSOS1D; IPTG-inducible

Depositor

David Savage

Article

Bonacci et al Proc Natl Acad Sci U S A. 2012 Jan 10;10

Insert

CbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D

Expression

Bacterial

Available Since

April 4, 2014

Availability

Academic Institutions and Nonprofits only

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pSR43.6r

Plasmid

#63197

Purpose

Expresses CcaS, Ho1, and PcyA constitutively

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

CcaS
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pBTK622

Plasmid

#180592

Purpose

tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

Depositor

Jeffrey Barrick

Article

Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

Inserts

tdk
kanR

Use

Synthetic Biology

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pmRNA-iSyn-EGFP

Plasmid

#225907

Purpose

In vitro transcription of cap-independently (synthetic IRES) translated mRNA encoding EGFP.

Depositor

Tom Brown

Article

Golojuch et al Nat Commun. 2025 Oct 16;16(1):9205. doi:

Inserts

PABP motif
HRV-B3-derived synthetic IRES
EGFP
HBA1 UTR

Use

Synthetic Biology; Mrna preparation

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pTlpA39-Wasabi

Plasmid

#86116

Purpose

mWasabi reporter gene gated by TlpA39

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA39
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

D341A, D135V, A217V, L236F

Promoter

pTlpA and pTlpA, LacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGNW2

Plasmid

#122086

Purpose

suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP22x-1A(sfGFP)

Plasmid

#126969

Purpose

Level 1 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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