We narrowed to 14,552 results for: eng
-
Plasmid#117852PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertmedium-high promoter, RBS, mRFP1
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
p168-759
Plasmid#62619PurposeePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)DepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBTRCk-pVhb-maACR
Plasmid#215651PurposepBBR1 Origin, KanR, with maACR gene under Vhb promoterDepositorInsertacyl-CoA reductase
TagsMaltose binding protein and His tagExpressionBacterialPromoterVhbAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-pVhb-vfFadBA
Plasmid#215650PurposepACYC Origin, CmR, with vfFadBA gene under Vhb promoterDepositorInsertthiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
ExpressionBacterialPromoterVhbAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTS1026-Tier1-PhCMV-3xNLS-iRFP670
Plasmid#169532PurposeTier-1 vector encoding PhCMV-driven iRFP670 that is localized to the nucleus (PhCMV-3xNLS-iRFP670-pA).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPCMV-driven nucleus-localized far-red fluorescent protein iRFP670
ExpressionMammalianPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH233-Tier1-PmPGK1-eYFP
Plasmid#169522PurposeTier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPPGK-driven enhanced yellow fluorescent protein
ExpressionMammalianPromoterPPGKAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2404-Tier1-PhCMV-NeoR
Plasmid#169496PurposeTier-1 vector encoding PhCMV-driven neomycin resistance gene NeoR (PhCMV-NeoR-pA).DepositorInsertPCMV-driven aminoglycoside 3'-phosphotransferase, APH 3' II
ExpressionMammalianPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH227-Tier1-OTetO7-PCMVmin2-SEAP
Plasmid#169563PurposeTier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-2-SEAP-pA).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttetracycline-controlled SEAP production
ExpressionMammalianPromoterTetO7-PCMVminAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH232-Tier1-PmPGK1-mRuby2
Plasmid#169523PurposeTier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPPGK-driven monomeric red fluorescent protein mRuby2
ExpressionMammalianPromoterPPGKAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM545-Tier1-PhCMV-Citrine
Plasmid#169505PurposeTier-1 vector encoding PhCMV-driven yellow fluorescent protein Citrine (PhCMV-Citrine-pA).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPCMV-driven yellow fluorescent protein citrine
ExpressionMammalianPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM585-Tier1-PhCMV-Igk
Plasmid#169486PurposeTier-1 vector encoding PhCMV-driven secretion signal Igk (PhCMV-Igk-pA).DepositorAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2379-Tier1-PhCMV-PEST
Plasmid#169484PurposeTier-1 vector encoding PhCMV-driven PEST tag for destabilization of proteins (PhCMV-PEST-pA).DepositorInsertPCMV-driven PEST degradation tag
ExpressionMammalianPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI-T7Max-UTR1-CTerminus8xHis-T500
Plasmid#178419PurposeEmpty backbone. T7Max promoter with C-His TagDepositorTypeEmpty backboneUseSynthetic BiologyTags8xHisExpressionBacterialPromoterT7MaxAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMST-710
Plasmid#118078PurposepmbfA::gRNA1(cexA)::ttrpCDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA1 (CexA)
PromoterpmbfAAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMST-711
Plasmid#118079PurposepmbfA::gRNA2(cexA)::ttrpCDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA2 (CexA)
PromoterpmbfAAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT3928
Plasmid#113331Purposecontrol for Hermes transposition; LEU2 without flanking Hermes TIRs, recoded transposase driven by TEF1 promoterDepositorInsertsbeta-isopropyl malate dehydrogenase
codon-optimized Hermes transposase
UseUnspecifiedAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
PHBA02
Plasmid#83536PurposeConstitutive TEF1 promoter mediated expression of the UBiC and ARO4* genesDepositorInsertpTEF1-ARO4*-CYC1t-pTEF1-UBiC-CYC1t
ExpressionYeastPromoterpTEF1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIVT-Flp-2A-BleoR
Plasmid#232752PurposeIVT template for making bicistronic modRNA encoding Flippase and Zeocin resistance geneDepositorInsertsFlpO
BleoR
UseIn vitro transcriptionAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-SpCas9-NRTH-P2A-EGFP (RMD21)
Plasmid#197503PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpCMV-T7-BPNLS-ABE8e-nSpCas9-NRTH-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA and nSpCas9-NRTH(D10A/I32…PromoterCMV and T7Available sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-AOX1-eGFP
Plasmid#126718Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
ExpressionYeastPromoterAOX1Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only