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Menghao Cai Lab: Engineered ethanol-driven biosynthetic system for improving production of acetyl-CoA derived drugs in Crabtree-negative yeast
Unpublished

Plasmids from Article

ID Plasmid Purpose  
126708pUC_delta ald1vector for knocking out ald1 in Komagataella phaffii
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126710pZ_P-GAP-ADH3 yeast plasmid complementing ADH3 from Komagataella phaffii
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126711pZ_P-GAP-ALD1 yeast plasmid complementing ALD1 from Komagataella phaffii
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126712pZ_P-GAP-ACS1 yeast plasmid complementing ACS1 from Komagataella phaffii
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126713pZ_P-ADH3-eGFPyeast plasmid overexpressing eGFP
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126714pZ_P-ALD-eGFPyeast plasmid overexpressing eGFP
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126715pZ_P-ACS1-eGFPyeast plasmid overexpressing eGFP
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126716pZ_P-ICL1-eGFPyeast plasmid overexpressing eGFP
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126717pZ_P-GAP-eGFPyeast plasmid overexpressing eGFP
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126718pZ_P-AOX1-eGFPyeast plasmid overexpressing eGFP
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126719pZ_P-HXT1-eGFPyeast plasmid overexpressing eGFP
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126720pZ_lacO-cP-AOX1-eGFPyeast plasmid overexpressing eGFP
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126721pZ_P-ICL1-LacI-Mit1ADLacI-Mit1AD on pPICZ B expression vector
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126722pZ_P-GAP-LacI-Mit1ADLacI-Mit1AD on pGAPZ_ A expression vector
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126723pZ_P-ICL1-LacI-VP16LacI-VP16 on pPICZ B expression vector
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126724pZ_P-ICL1-LacI-Gal4ADLacI-Gal4AD on pPICZ B expression vector
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126725pK_-P-GAP-NpgA+AtXyeast plasmid overexpressing AtX and NpgA
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126727pK_lacO-cP-AOX1-AtX+NpgAyeast plasmid overexpressing AtX and NpgA
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126728pZ_lacO-cP-AOX1-LovByeast plasmid overexpressing LovB
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126729pZ_lacO-cP-AOX1-LovCyeast plasmid overexpressing LovC
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126731pZ_lacO-cP-AOX1-NpgAyeast plasmid overexpressing NpgA
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126734pKdH_P-GAP-ScADH2yeast plasmid overexpressing ADH2 from Saccharomyces cerevisiae
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126736pKdH_P-GAP-ScACS1*yeast plasmid overexpressing ACS1 from Saccharomyces cerevisiae
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126737pKdH_P-GAP-ScADH2+P-GAP*-ScALD6yeast plasmid overexpressing ADH2 and ALD6 from Saccharomyces cerevisiae
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126738pKdH_P-GAP-ScADH2+P-GAP*-ScACS1*yeast plasmid overexpressing ADH2 and ACS1 from Saccharomyces cerevisiae
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126739pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1*yeast plasmid overexpressing ADH2,ALD6 and ACS1 from Saccharomyces cerevisiae
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126740pK_P-GAP-ACC1*yeast plasmid overexpressing ACC1 from Komagataella phaffii
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126741pZ_lacO-cP-AOX1-sLovAyeast plasmid overexpressing sLovA
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126742pZ_lacO-cP-AOX1-CPRyeast plasmid overexpressing CPR
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126743pK_lacO-cP-AOX1-sARyeast plasmid overexpressing CPR and sLovA
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126744pK_P-ICL1-LM_lacO-cP-AOX1-sARyeast plasmid overexpressing sLovA,CPR and LacI-Mit1AD
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126745pK_P-GAP-LM_lacO-cP-AOX1-sARyeast plasmid overexpressing sLovA,CPR and LacI-Mit1AD
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126746pZ_P-HXT1-FAS1upvector for replacing the promoter of FAS1 coding gene
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126747pAG_P-HXT1-FAS1upvector for replacing the promoter of FAS1 coding gene
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Recombinant Antibodies from Article