Engineered ethanol-driven biosynthetic system for improving production of acetyl-CoA derived drugs in Crabtree-negative yeast.
Liu Y, Bai C, Liu Q, Xu Q, Qian Z, Peng Q, Yu J, Xu M, Zhou X, Zhang Y, Cai M
Metab Eng. 2019 Jul;54:275-284. doi: 10.1016/j.ymben.2019.05.001. Epub 2019 May 9. PubMed Article
Liu Y, Bai C, Liu Q, Xu Q, Qian Z, Peng Q, Yu J, Xu M, Zhou X, Zhang Y, Cai M
Metab Eng. 2019 Jul;54:275-284. doi: 10.1016/j.ymben.2019.05.001. Epub 2019 May 9. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 126708 | pUC_delta ald1 | vector for knocking out ald1 in Komagataella phaffii |
| 126710 | pZ_P-GAP-ADH3 | yeast plasmid complementing ADH3 from Komagataella phaffii |
| 126711 | pZ_P-GAP-ALD1 | yeast plasmid complementing ALD1 from Komagataella phaffii |
| 126712 | pZ_P-GAP-ACS1 | yeast plasmid complementing ACS1 from Komagataella phaffii |
| 126713 | pZ_P-ADH3-eGFP | yeast plasmid overexpressing eGFP |
| 126714 | pZ_P-ALD-eGFP | yeast plasmid overexpressing eGFP |
| 126715 | pZ_P-ACS1-eGFP | yeast plasmid overexpressing eGFP |
| 126716 | pZ_P-ICL1-eGFP | yeast plasmid overexpressing eGFP |
| 126717 | pZ_P-GAP-eGFP | yeast plasmid overexpressing eGFP |
| 126718 | pZ_P-AOX1-eGFP | yeast plasmid overexpressing eGFP |
| 126719 | pZ_P-HXT1-eGFP | yeast plasmid overexpressing eGFP |
| 126720 | pZ_lacO-cP-AOX1-eGFP | yeast plasmid overexpressing eGFP |
| 126721 | pZ_P-ICL1-LacI-Mit1AD | LacI-Mit1AD on pPICZ B expression vector |
| 126722 | pZ_P-GAP-LacI-Mit1AD | LacI-Mit1AD on pGAPZ_ A expression vector |
| 126723 | pZ_P-ICL1-LacI-VP16 | LacI-VP16 on pPICZ B expression vector |
| 126724 | pZ_P-ICL1-LacI-Gal4AD | LacI-Gal4AD on pPICZ B expression vector |
| 126725 | pK_-P-GAP-NpgA+AtX | yeast plasmid overexpressing AtX and NpgA |
| 126727 | pK_lacO-cP-AOX1-AtX+NpgA | yeast plasmid overexpressing AtX and NpgA |
| 126728 | pZ_lacO-cP-AOX1-LovB | yeast plasmid overexpressing LovB |
| 126729 | pZ_lacO-cP-AOX1-LovC | yeast plasmid overexpressing LovC |
| 126731 | pZ_lacO-cP-AOX1-NpgA | yeast plasmid overexpressing NpgA |
| 126734 | pKdH_P-GAP-ScADH2 | yeast plasmid overexpressing ADH2 from Saccharomyces cerevisiae |
| 126736 | pKdH_P-GAP-ScACS1* | yeast plasmid overexpressing ACS1 from Saccharomyces cerevisiae |
| 126737 | pKdH_P-GAP-ScADH2+P-GAP*-ScALD6 | yeast plasmid overexpressing ADH2 and ALD6 from Saccharomyces cerevisiae |
| 126738 | pKdH_P-GAP-ScADH2+P-GAP*-ScACS1* | yeast plasmid overexpressing ADH2 and ACS1 from Saccharomyces cerevisiae |
| 126739 | pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1* | yeast plasmid overexpressing ADH2,ALD6 and ACS1 from Saccharomyces cerevisiae |
| 126740 | pK_P-GAP-ACC1* | yeast plasmid overexpressing ACC1 from Komagataella phaffii |
| 126741 | pZ_lacO-cP-AOX1-sLovA | yeast plasmid overexpressing sLovA |
| 126742 | pZ_lacO-cP-AOX1-CPR | yeast plasmid overexpressing CPR |
| 126743 | pK_lacO-cP-AOX1-sAR | yeast plasmid overexpressing CPR and sLovA |
| 126744 | pK_P-ICL1-LM_lacO-cP-AOX1-sAR | yeast plasmid overexpressing sLovA,CPR and LacI-Mit1AD |
| 126745 | pK_P-GAP-LM_lacO-cP-AOX1-sAR | yeast plasmid overexpressing sLovA,CPR and LacI-Mit1AD |
| 126746 | pZ_P-HXT1-FAS1up | vector for replacing the promoter of FAS1 coding gene |
| 126747 | pAG_P-HXT1-FAS1up | vector for replacing the promoter of FAS1 coding gene |