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pKR85:H2A1p-BD7-AS (30bp)2mm(12-13)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251293

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR87:H2A1p-BD8-AS (30bp)2mm(12-13)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251295

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR89:H2A1p-BD9-AS (30bp)2mm(12-13)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251297

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR91:H2A1p-BD10-AS (30bp)2mm(12-13)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251299

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR71:H2A1p-(linker2)-AFP BD-AS(50bp)2mm(18-19)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251283

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR72:H2A1p-AFP BD-AS(50bp)2mm(18-19)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251284

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pTrcHisbglk

Plasmid

#252958

Purpose

Expresses beta glucoside kinase in E. coli

Depositor

John Thompson

Article

Thompson et al J Biol Chem. 2002 Sep 13;277(37):34310-2

Insert

beta glucoside kinase

Tags

His

Expression

Bacterial

Promoter

trc

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 WT 30mer

Plasmid

#231888

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WT 30mer

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 30mer

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_DBD

Plasmid

#231886

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 DBD

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 DBD

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 WW+

Plasmid

#231885

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WW+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

T7

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 Swap1

Plasmid

#231879

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Swap1

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Swap1

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 R3

Plasmid

#231874

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 R3

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R3

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103R, D133R, K143R

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 P+

Plasmid

#231872

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 P+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 P+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S122P, T131F, A141P, E143D

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 K5

Plasmid

#231866

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 K5

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 K5

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103K, D115K, D127K, D133K, E143K

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 E3

Plasmid

#231863

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 E3

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 E3

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D105E, D118E, D139E

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 W120A

Plasmid

#231862

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 W120A

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

W120A

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Med15

Plasmid

#231887

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Med15

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Med15

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 R3 W

Plasmid

#231876

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 R3 W

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R3 W

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103R, N126W, D133R, K143R

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 R2

Plasmid

#231870

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 R2

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R2

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

K118R, K140R

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 L123A+

Plasmid

#231869

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 L123A+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 L123A+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L123A

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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