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pACU2

Plasmid

#31223

Depositor

Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Type

Empty backbone

Expression

Insect

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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pV1382

Plasmid

#111436

Purpose

S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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LAP2 Full I pAcGFP-N1 monomeric GFP (1317)

Plasmid

#62044

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

LAP2beta (Tmpo Rat)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2

Plasmid

#164514

Purpose

Green glucose sensor with mRuby2 fusion, cytosolic. The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2.mRuby3 (Plasmid #244084) instead of this plasmid.

Depositor

Loren Looger

Article

Keller et al Cell Rep. 2021 Jun 22;35(12):109284. doi

Insert

(Cyto)iGlucoSnFR-mRuby2

Use

AAV

Mutation

Synthetic Construct

Promoter

GFAP

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mCherry-CaM

Plasmid

#127393

Purpose

Fluorescent reporter for calmodulin 1

Depositor

Ryohei Yasuda

Article

Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

Insert

Calmodulin 1 (Calm1 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

pCAG

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(Cyto)iGlucoSnFR

Plasmid

#164513

Purpose

Green glucose sensor, cytosolic. The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2 (Plasmid #244111) instead of this plasmid.

Depositor

Loren Looger

Article

Keller et al Cell Rep. 2021 Jun 22;35(12):109284. doi

Insert

(Cyto)iGlucoSnFR

Use

AAV

Mutation

Synthetic Construct

Promoter

GFAP

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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LAP2 Full I pAcGFP-N1 monomeric GFP (1317)

Plasmid

#62044

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

LAP2beta (Tmpo Rat)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2

Plasmid

#164514

Purpose

Green glucose sensor with mRuby2 fusion, cytosolic. The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2.mRuby3 (Plasmid #244084) instead of this plasmid.

Depositor

Loren Looger

Article

Keller et al Cell Rep. 2021 Jun 22;35(12):109284. doi

Insert

(Cyto)iGlucoSnFR-mRuby2

Use

AAV

Mutation

Synthetic Construct

Promoter

GFAP

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarG

Plasmid

#31221

Depositor

Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Insert

CD4-tdGFP (CD4 Fly, Aequorea victoria, Human)

Tags

CD4 and EGFP/GFP

Expression

Insect

Mutation

Fusion of signal peptide of Drosophila Akh gene, …

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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FGFR2 3xFlag

Plasmid

#110063

Purpose

FGFR2 expression in mammalian cells (JCOPCO paper)

Depositor

Martin Fernandez-Zapico

Article

Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(Cyto)iGlucoSnFR

Plasmid

#164513

Purpose

Green glucose sensor, cytosolic. The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2 (Plasmid #244111) instead of this plasmid.

Depositor

Loren Looger

Article

Keller et al Cell Rep. 2021 Jun 22;35(12):109284. doi

Insert

(Cyto)iGlucoSnFR

Use

AAV

Mutation

Synthetic Construct

Promoter

GFAP

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pBS PKC theta

Plasmid

#8426

Depositor

Frederic Mushinski

Article

Mischak et al FEBS Lett 1993 Jul 12;326(1-3):51-5.

Insert

Mouse Protein Kinase C theta (Prkcq Mouse)

Expression

Bacterial

Available Since

Nov. 22, 2005

Availability

Academic Institutions and Nonprofits only

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pUC-GFP-AT

Plasmid

#133306

Purpose

"Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisation

Depositor

Chris Barnes

Article

Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

Insert

axe/txe toxin-antitoxin cassette

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarG

Plasmid

#31221

Depositor

Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Insert

CD4-tdGFP (CD4 Human, Fly, Aequorea victoria)

Tags

CD4 and EGFP/GFP

Expression

Insect

Mutation

Fusion of signal peptide of Drosophila Akh gene, …

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
FGFR2 3xFlag

Plasmid

#110063

Purpose

FGFR2 expression in mammalian cells (JCOPCO paper)

Depositor

Martin Fernandez-Zapico

Article

Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pBS PKC theta

Plasmid

#8426

Depositor

Frederic Mushinski

Article

Mischak et al FEBS Lett 1993 Jul 12;326(1-3):51-5.

Insert

Mouse Protein Kinase C theta (Prkcq Mouse)

Expression

Bacterial

Available Since

Nov. 22, 2005

Availability

Academic Institutions and Nonprofits only

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pUC-GFP-AT

Plasmid

#133306

Purpose

"Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisation

Depositor

Chris Barnes

Article

Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

Insert

axe/txe toxin-antitoxin cassette

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-2xFYVE_hrs

Plasmid

#67676

Purpose

Multisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrs

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

2xFYVEhrs

Use

Gateway multisite 3' entry clone

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-mEGFP-CaMKIIa

Plasmid

#127389

Purpose

Fluorescent reporter for Ca2+/calmodulin-dependent protein kinase II alpha

Depositor

Ryohei Yasuda

Article

Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)

Tags

mEGFP

Expression

Mammalian

Promoter

pCAG

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-2xFYVE_hrs

Plasmid

#67676

Purpose

Multisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrs

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

2xFYVEhrs

Use

Gateway multisite 3' entry clone

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge