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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 254 results for: sms

Showing: 1 - 20 of 254 results

  1. Five Popular Model Organisms

    Type
    Blog Post
    ...Morgan Model organisms are vital tools used by researchers around the globe. These organisms share many ...molecular mechanisms underlying the disease (Menezes et al., 2015). As yeast are unicellular organisms, they...Looking for more model organisms? Check out Part two in the popular model organisms series, and read about...this blog post, we’ll cover five popular model organisms, but there are many more out there. The house...physiologically similar to humans than many other model organisms, they are often used to study human diseases. ... rest of the body than studies in other model organisms or cell culture. For example, Scott Lowe’s lab...at Addgene. Another advantage of mice as model organisms is that they are genetically tractable. Mice can...

  2. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...covered five model organisms in a previous blog post, but here are five more model organisms that have left...emerging model organisms!) Escherichia coli One of the most recognizable and utilized model organisms is Escherichia...: Read more about model organisms in the first Five Popular Model Organisms blog post Read more about... biological processes and developments. Model organisms often feature genetic, physiological, or developmental...Mexican axolotl is one of the most unique model organisms, recognized for its incredible regeneration abilities... create fluorescent fusions in a multitude of organisms including Xenopus. Overall, the ability for scientists...one of, if not the most, recognized plant model organisms in the world. With its extensive scientific history...

  3. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...populations of transgenic laboratory organisms but not wild organisms. For example, an sgRNA-only drive ... Medical School. Scientists making transgenic organisms with Cas9 should be aware of the potential hazards... extreme caution. Why does making transgenic organisms with Cas9 risk creating a gene drive? The simplest...experiments in a geographic area where escaped organisms won't be able to find mates2. For example, ongoing... confinement involves working with laboratory organisms that can't reproduce with wild ones. For example... gene drive experiments are less hazardous in organisms that seldom reproduce sexually because the drive...spread. Barrier confinement seeks to keep the organisms in the laboratory. It varies by organism, but ...

  5. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...PubMed PMID: 12665802. 2. Branda, CS and Dymecki, SM. Talking about a Revolution: The Impact of Site-Specific...14723844. 3. Atasoy D, Aponte Y, Su HH, and Sternson SM. 2008. A FLEX switch targets Channelrhodopsin-2 to...

  7. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...Sullivan JS, Roche M, Zaunders JJ, Gabuzda D, Crowe SM, Mills J, Lewin SR, Brew BJ, Cunningham AL, Churchill... virus used. All of these expression control mechanisms require a specific modification of the viral ...specifically AAV, are also being exploited as delivery mechanisms for gene therapy. The tractability of AAV, low...

  11. Chemogenetics Guide

    Type
    Guide
    ...19607790 (Link opens in a new window) Atasoy D, Sternson SM (2018). Chemogenetic Tools for Causal Cellular and... Lee PH, Atasoy D, Su HH, Looger LL, and Sternson SM (2011). Chemical and genetic engineering of selective..., Hu X, Galvan A, Basu J, Michaelides M, Sternson SM (2019). Ultrapotent chemogenetics for research and...PMID: 11402033 (Link opens in a new window) Sternson SM, Roth BL (2014). Chemogenetic tools to interrogate...

  13. Pipetting Protocol

    Type
    Protocol
    ...different types of pipettes that have different mechanisms to disperse liquids, this protocol is focused...

  14. Pouring LB Agar Plates

    Type
    Protocol
    ... you are working with any weird and wonderful organisms. While your samples are sterilizing in the autoclave...

  15. CRISPR Guide

    Type
    Collection
    ...knock out target genes in various cell types and organisms, but modifications to various Cas enzymes have... In this section, we will introduce the basic mechanisms of CRISPR and some of the ways the CRISPR components...efficiency, or flexibility of the Cas enzyme. Basic Mechanisms of CRISPR The requirements for a CRISPR knockout...Examples of increased fidelity enzymes, and their mechanisms, include: eSpCas9(1.1) - weaken interactions ...family members inhibit CRISPR by a variety of mechanisms, including blocking crRNA loading or PAM recognition...different locations, by combining other gene editing mechanisms, including transposons, integrases, and recombinases...be added to detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar...

  16. Microbiology Resources

    Type
    Collection
    ...these model organisms. See below for plasmid kits and other resources relevant to these organisms, or use ...depositors are making this possible in a range of organisms. Find CRISPR tools for use in genome editing of...

  17. CRISPR References and Information

    Type
    Collection
    ...Supports a range of Cas enzymes (Cas9 and Cas12) and organisms. Developed by the Jin-Soo Kim Lab . Cas-Designer...target site common to a set of similar sequences. Organisms include human, mouse, rat, chicken, frog, zebrafish...upload. Currently supports a wide selection of organisms. From the Database Center for Life Science (DBCLS...

  18. Mammalian RNAi Tools

    Type
    Collection
    .... W., & Sontheimer, E. J. (2009). Origins and mechanisms of miRNAs and siRNAs . Cell, 136 (4), 642–655...Abadi, S. J., & Gharagouzlo, E. (2017). Molecular mechanisms and biological functions of siRNA . International...

  19. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...Viral Pseudotyping SARS-CoV-2 Pseudotyped Virus Mechanisms of SARS-CoV-2 infection remain poorly understood...This approach allows researchers to safely study mechanisms of infection and to determine methods of neutralizing...

  20. p53 Pathway

    Type
    Collection
    ...characterized as gain-of-function, expanding the mechanisms by which mutant p53 is known to function in cancer...Thrombospondin 1 Return to top References Unravelling mechanisms of p53-mediated tumour suppression. Bieging KT...
Showing: 1 - 20 of 254 results