The AAV Education and Development (AAVED) Series
AAV Technologies and Methods for Targeted Neuronal Manipulation
The goal of this year’s workshop is to brainstorm practical strategies for using combinations of AAV-based molecular tools to achieve targeted neuronal manipulation (aka intersectional control). The end result of the meeting will be a publicly shared practical guide for all scientists in the field and every attendee of the meeting will be a co-author of the guide. We hope that this guide will improve technology adoption and scientific outcomes.
Event Details
- Date: Friday September 21, 2018, 9 AM - 5 PM
- Location: Addgene, 75 Sidney St, Cambridge, MA, 02139
Organizing Committee
- Chair: Dr. Melina Fan, Co-Founder and CSO, Addgene
- Organizing Committee: Dr. Constance Cepko, Harvard Medical School
Dr. Melina Fan, Dr. Susanna Bachle, Dr. Karen Guerin, Dr. Leila Haery, Dr. Meghan Rego, Addgene
Confirmed Speakers
- Constance Cepko Harvard Medical School
- Ali Cetin Allen Institute for Brain Science
- Ben Deverman Broad Institute
- Katherine Matho Cold Spring Harbor Laboratory
- Gowan Tervo Janelia Research Campus
- Sarada Viswanathan Janelia Research Campus
- Kenton Woodard Penn Vector Core
Melina Fan, Karen Guerin, and Leila Haery from Addgene will also be speaking or leading workshops.
Tentative Meeting Agenda
Thursday, September 20
| 6:00 PM-8:00 PM | Group Dinner Relax and Connect with Fellow Speakers and Attendees |
Friday, September 21
| 9:00 AM | Breakfast and Welcome Melina Fan |
| 9:30 AM | Cre and Flpo Katherine Matho |
| 10:15 AM | AAV Retrograde Tracing Gowan Tervo and Sarada Viswanathan |
| 11:00 AM | Circuit Tracing Ali Cetin |
| 12:00 PM | Lunch |
| 1:00 PM | Promoter/Enhancer Specificity, Serotype specificity, AAV dosing, and Other Forms of Regulation Ben Deverman |
| 2:00 PM | Cre-DOG and Combinatorial Strategies Connie Cepko |
| 2:45 PM | Break |
| 3:00 PM | (Group Discussion) Technologies and Resources Missing in the Field Kenton Woodard |
| 4:00 PM | Wrap Up & Next Steps Melina Fan/Leila Haery |
| 5:00 PM | Adjourn |
Meeting Preparation
For Speakers:
Each speaker will give a 20 minute presentation, followed by 20 minutes of questions/discussion. The goal of the presentation is to:
- Give a general overview of the technology and how it can be used for targeted neuronal manipulation. Topics may include:
- How this technology is used for targeted neuronal manipulation.
- Practical tips for using the technology in the lab (e.g., controls to perform, recommended histology conditions, minimum/maximum incubation time after in vivo delivery, etc.)
- Spark the discussion focused on:
- Potential combination with other technologies to achieve different experimental goals.
- How does this method compare to other similar methods? Where does this method fit in the big picture of methods for targeted neuronal manipulation?
- Best practices for using this techonology in the lab (main issues/factors that researchers should consider when planning experiments).
- What resources might be useful to help improve this technology or its application? What are some current obstacles that researchers face right now (e.g., not enough protocols, cost, not enough good antibodies, access to reagents)?
For All Attendees:
To help us best prepare and compile resources for this meeting's publication, we are asking participants to share practical information in the form of data and protocols showing examples of AAV strategies for intersectional control. Please see our list of suggested materials below.
- Suggested materials to share:
- List of papers in which your technology (that your group is presenting on) is used to achieve targeted neuronal manipulation.
- Figures, tables, data, or images on best practices (e.g., dose response data, list of antibodies and recommended dilutions, etc.), novel applications, or any other useful information.
- Experimental protocols or schematics.
- Examples of research materials to share:
- In vivo data: Retrograde hSyn1-GCaMP6f-P2A-nls-dTomato
- Detailed protocol: AAV Production in HEK293 Cells
- Please share materials by uploading to your Dropbox link below (now closed) (only you and Addgene have access to your individual folder and your materials will not be shared without your permission). Materials don't have to be organized (yet), we will take care of that. Note that you will have final approval before anything is released publicly, but please only share materials that you are comfortable and willing to share. Please only share materials that you have permission to share (e.g., publication's permission).
- Connie Cepko, Harvard Medical School
- Ali Cetin and Shenqin Yao, Allen Institute
- Ben Deverman, Broad Institute
- Katherine Matho, Cold Spring Harbor Laboratory
- Gowan Tervo and Sarada Viswanathan, Janelia Research Campus
- Kenton Woodard, Penn Vector Core
Intended Outcome of Meeting
The outcome of this meeting will be an openly shared communication representing a reference for AAV use in neuronal intersectional control.
Through your participation in this meeting, you will be a co-author on this communication and you will be helping scientists successfully adopt these tools. Thank you for your contributions and your commitment to open science! Please see the meeting preparation section for how to best help contribute to this publication.
Citation information for openly shared communication:
- Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation. Haery L, Deverman BE, Matho KS, Cetin A, Woodard K, Cepko C, Guerin KI, Rego MA, Ersing I, Bachle SM, Kamens J, Fan M. Front Neuroanat. 2019 Nov 26;13:93.doi: 10.3389/fnana.2019.00093. PubMed 31849618