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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9 results for: sms

Showing: 1 - 9 of 9 results

  1. Chemogenetics Guide

    Type
    Guide
    ...19607790 (Link opens in a new window) Atasoy D, Sternson SM (2018). Chemogenetic Tools for Causal Cellular and... Lee PH, Atasoy D, Su HH, Looger LL, and Sternson SM (2011). Chemical and genetic engineering of selective..., Hu X, Galvan A, Basu J, Michaelides M, Sternson SM (2019). Ultrapotent chemogenetics for research and...PMID: 11402033 (Link opens in a new window) Sternson SM, Roth BL (2014). Chemogenetic tools to interrogate...

  2. CRISPR Guide

    Type
    Guide
    ...knock out target genes in various cell types and organisms, but modifications to various Cas enzymes have... In this section, we will introduce the basic mechanisms of CRISPR and some of the ways the CRISPR components...efficiency, or flexibility of the Cas enzyme. Basic Mechanisms of CRISPR The requirements for a CRISPR knockout...Examples of increased fidelity enzymes, and their mechanisms, include: eSpCas9(1.1) - weaken interactions ...family members inhibit CRISPR by a variety of mechanisms, including blocking crRNA loading or PAM recognition...different locations, by combining other gene editing mechanisms, including transposons, integrases, and recombinases...be added to detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar...

  3. Modular Cloning Guide

    Type
    Guide
    ...at 18 levels for rapid characterization in new organisms. Mobius Assembly Vector Toolkit Bacterial Expression...reconstituting the ubiquitination cascades of different organisms in E. coli . MetClo DNA Assembly Kit Bacterial...with various delivery approaches, enzymes, and mechanisms. zCas9i cloning kit: MoClo-compatible CRISPR/...Platinum TALENs for use in a wide variety of target organisms. TALEN Kit Genome Engineering, TALEN Stephen Ekker...fusion proteins for use in many different model organisms. Diatom uLoop Assembly Kit Other, Yeast Expression...tags, and signal peptides, for use in a range of organisms including diatoms, yeast, plants and bacteria....

  4. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...AAV vectors . Microorganisms, 12 (2), 384. https://doi.org/10.3390/microorganisms12020384 (Link opens in...later reconstructed within the cell via different mechanisms. One of these methods uses splice site donor ...

  5. Plan Your Experiment

    Type
    Guide
    ...researchers can adapt these experiments for different organisms. This page provides a general framework to get...principles also apply to using CRISPR in other organisms. As with most experiments, you first need to have...

  6. Molecular Biology Reference

    Type
    Guide
    ...machinery from a particular organism or group of organisms. Meaning, if a plasmid in intended for use in ...can drive gene expression in a wide variety of organisms, including plants, worms, mice, and even cultured...

  7. Guide to Using Pooled Libraries

    Type
    Guide
    ...being studied. Examples of published selection mechanisms for lentiviral CRISPR libraries include: resistance...

  8. Optogenetics Guide

    Type
    Guide
    ...and study certain behavioral responses in model organisms like mice, zebrafish, and Drosophila. These tools...

  9. Adenovirus Guide

    Type
    Guide
    ...G). Different serotypes exhibit varying tissue tropisms, meaning they preferentially infect certain tissues...
Showing: 1 - 9 of 9 results