We narrowed to 9 results for: sms

..., Hu X, Galvan A, Basu J, Michaelides M, Sternson SM (2019). Ultrapotent chemogenetics for research and...

...knock out target genes in various cell types and organisms, but modifications to various Cas enzymes have... In this section, we will introduce the basic mechanisms of CRISPR and some of the ways the CRISPR components...efficiency, or flexibility of the Cas enzyme. Basic Mechanisms of CRISPR The requirements for a CRISPR knockout...Examples of increased fidelity enzymes, and their mechanisms, include: eSpCas9(1.1) — weaken interactions ...family members inhibit CRISPR by a variety of mechanisms, including blocking crRNA loading or PAM recognition...different locations, by combining other gene editing mechanisms, including transposons, integrases, and recombinases...be added to detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar...

...at 18 levels for rapid characterization in new organisms. Mobius Assembly Vector Toolkit Bacterial Expression...reconstituting the ubiquitination cascades of different organisms in E. coli . MetClo DNA Assembly Kit Bacterial...with various delivery approaches, enzymes, and mechanisms. zCas9i cloning kit: MoClo-compatible CRISPR/...Platinum TALENs for use in a wide variety of target organisms. TALEN Kit Genome Engineering, TALEN Stephen Ekker...fusion proteins for use in many different model organisms. Diatom uLoop Assembly Kit Other, Yeast Expression...tags, and signal peptides, for use in a range of organisms including diatoms, yeast, plants and bacteria....

...AAV vectors . Microorganisms, 12 (2), 384. https://doi.org/10.3390/microorganisms12020384 (Link opens in...later reconstructed within the cell via different mechanisms. One of these methods uses splice site donor ...

...researchers can adapt these experiments for different organisms. This page provides a general framework to get...principles also apply to using CRISPR in other organisms. As with most experiments, you first need to have...

...being studied. Examples of published selection mechanisms for lentiviral CRISPR libraries include: resistance...

...and study certain behavioral responses in model organisms like mice, zebrafish, and Drosophila. These tools...

...G). Different serotypes exhibit varying tissue tropisms, meaning they preferentially infect certain tissues...

...can drive gene expression in a wide variety of organisms, including plants, worms, mice, and cultured human...