We narrowed to 11,815 results for: 110

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tFucci(SA)5

Plasmid

#153520

Purpose

New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.

Depositor

Atsushi Miyawaki

Article

Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

Insert

tFucci(SA)5.

Tags

AzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Blast-PIP-FUCCI

Plasmid

#138715

Purpose

Lentiviral expression of the cell cycle marker PIP-FUCCI

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-FUCCI

Use

Lentiviral

Tags

mCherry and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Hygro-PIP-FUCCI

Plasmid

#138714

Purpose

Lentiviral expression of the cell cycle marker PIP-FUCCI

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-FUCCI

Use

Lentiviral

Tags

mCherry and mVenus

Expression

Mammalian

Promoter

hPGK

Available Since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

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tFucci(CA)5pPBbsr2

Plasmid

#197351

Purpose

Encoding tFucci(CA)5.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

tFucci(CA)5

Tags

AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-GFP-SF3B6-FLAG

Plasmid

#86870

Purpose

To express SF3B6 with a GFP and FLAG tags at N- and C-terminus, respectively.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

SF3B6 (SF3B6 Human)

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pHB4

Plasmid

#53462

Purpose

This plasmid contains an ADHpr driven chimeric protein with SNC1, N-terminal GFP and C-terminal SUC2. It contains flanking homology to SUC2, allowing optional integration.

Depositor

Elizabeth Conibear

Article

Burston et al J Cell Biol. 2009 Jun 15. 185(6):1097-11

Insert

yeGFP-Snc1-Suc2

Expression

Yeast

Promoter

ADH

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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Nanog-t2A-mCherry

Plasmid

#127541

Purpose

Expresses the transactivation domain of mouse Nanog and mCherry via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

Nanog (Nanog Mouse)

Expression

Mammalian

Mutation

Codes for the last 110 a.a of Nanog

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-Fluc-CG10596_E

Plasmid

#146189

Purpose

Insect Expression of CG10596-3UTR

Depositor

Elisa Izaurralde

Article

Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

Insert

CG10596-3UTR (Msr-110 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAT9752-BEAR-mScarlet

Plasmid

#162991

Purpose

BEAR target plasmid with split mScarlet and disrupted 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

mScarlet split with an intron between amino acids 110-111

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-Evectin-2

Plasmid

#221754

Purpose

Stable expression of Evectin-2 in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

PLEKHB2 (PLEKHB2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

tandem PLEKHB2(PH domain) (amino acids 1-110)

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-GFP-SF3B6-Delete-NLS-FLAG

Plasmid

#86871

Purpose

To express SF3B6 delete NLS signal with a GFP and FLAG tags at N- and C-terminus, respectively.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

SF3B6 (SF3B6 Human)

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

amino acids 7-29 deletion

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAT9753-BEAR-mScarlet-preedited

Plasmid

#162994

Purpose

BEAR control plasmid with split mScarlet and intact 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

mScarlet split with an intron between amino acids 110-111

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG FUCCI

Plasmid

#136934

Purpose

donor plasmid for constitutive FUCCI expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

Use

Synthetic Biology

Tags

Clover and mKO2

Expression

Mammalian

Promoter

CAG

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-TA Bik-IRES-Puromycin

Plasmid

#133026

Purpose

Expresses EGFP-tagged tail anchor sequence of Bik

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Bcl-2-interacting killer (BIK Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-110

Promoter

Human elongation factor 1 alpha

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR

Plasmid

#221447

Purpose

Lentiviral mammalian expression vector of eGFP-tagged NFATC2IP ORF that is fused with S-phase restricted (SPR) degron at its C-terminus

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

SPR degron: hGEM (1-110) fragment that is fused t…

Promoter

CMV

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MTK3_017

Plasmid

#123732

Purpose

Encodes Fluorescent probe for G1/S transition as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Citrine::Geminin(1-110)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Tpa1(21-644)

Plasmid

#23140

Depositor

SeWon Suh

Article

Kim et al Nucleic Acids Res. 2010 Apr;38(6):2099-1

Insert

Tpa1 (TPA1 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-20

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
pBOB-EF1-FastFUCCI-Puro

Plasmid

#86849

Purpose

3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection

Depositor

Kevin Brindle, Duncan Jodrell

Article

Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164

Inserts

mKO2-hCDT1(30-120)
mAG-hGEM(1-110)
pac (puromycin N-acetyl-transferase)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1 and PGK

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiFUCCI(CA)5

Plasmid

#223176

Purpose

Lentiviral fluorescent ubiquitination-based cell cycle indicator (FUCCI)

Depositor

Neville Sanjana

Article

Liang et al Cell. 2024 Dec 26;187(26):7637-7654.e29.

Insert

FUCCI(CA)5

Use

Lentiviral

Tags

AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

Expression

Mammalian

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pTMiniTrex-mCherry-Shark1

Plasmid

#233143

Purpose

Serves as a template plasmid for amplifying Shark1 (Tet-OFF) hammerhead aptazyme-3xTy-tagging cassette

Depositor

Ronald Etheridge

Article

Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

Inserts

mCherry
Shark1

Use

Prokaryotic expression

Tags

3x Ty tag

Mutation

Codon optimized for Trypanosoma cruzi and changed…

Available Since

March 25, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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