161,492 results

1. pMK425 (GAL-OsTIR1(F74A))

   Plasmid

   #140657

   Purpose

   GAL-OsTIR1(F74A)

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   GAL-OsTIR1(F74A)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJW2098

   Plasmid

   #163094

   Purpose

   CRISPR repair template: add insertion site specific homology arms by PCR before insertion

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Silent mutations to remove piRNA sites

   Promoter

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ubiquitin WT

   Plasmid

   #12647

   Depositor

   Rachel Klevit

   Article

   Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

   Insert

   Ubiquitin - wild type (UBC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRPA32-mCherry

   Plasmid

   #211384

   Purpose

   mammalian expression of human RPA32 C-terminally fused to mCherry

   Depositor

   Helmut Pospiech

   Insert

   RPA32 (RPA2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2+ GCamp6-3NLS

   Plasmid

   #165401

   Purpose

   Expresses a nuclear localized genetically encoded calcium reporter.

   Depositor

   Philipp Niethammer

   Article

   Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

   Insert

   Nuclear Localization Signal
   

   Use

   Avian, xenopus, zebrafish

   Tags

   GCamp6

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEB-Tg

   Plasmid

   #28220

   Depositor

   Linzhao Cheng

   Article

   Chou et al Cell Res. 2011 Jan 18. ():.

   Insert

   SV40 large T
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-EEA1 wt

   Plasmid

   #42307

   Depositor

   Silvia Corvera

   Article

   Lawe et al J Biol Chem. 2000 Feb 4;275(5):3699-705.

   Insert

   EEA1 (EEA1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEP4 E02S EN2L

   Plasmid

   #20922

   Purpose

   used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Lin28

   Depositor

   James Thomson

   Article

   Yu et al Science. 2009 Mar 26.

   Insert

   Oct4 and Sox2; Nanog and Lin28 (POU5F1, SOX2, NANOG, LIN28A Human)
   

   Use

   Episomal/ebna

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIDK-HDAC1fl

   Plasmid

   #109158

   Purpose

   Encodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression system

   Depositor

   Monika Golas

   Article

   Inui et al Protein Sci. 2017 May;26(5):997-1011. do

   Insert

   human full-length HDAC1, sequence-optimized for insect cells (HDAC1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   p10

   Available since

   Jan. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti6.3-hEPO

    Plasmid

    #50436

    Purpose

    express human erythropoietin using lentiviral delivery system

    Depositor

    Juan Melero-Martin

    Article

    Lin et al Blood. 2011 Nov 17;118(20):5420-8. doi:

    Insert

    EPO (EPO Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

    Plasmid

    #64512

    Purpose

    Expression of St1 dCas9-3XTagBFP2 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-endo-Nb37

    Plasmid

    #205514

    Purpose

    Endosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    endo-Nb37
    

    Use

    Tags

    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-ICUE3

    Plasmid

    #61622

    Purpose

    FRET-based biosensor for monitoring cAMP

    Depositor

    Jin Zhang

    Article

    DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

    Insert

    ICUE3 (RAPGEF3 Human)
    

    Use

    Tags

    ECFP and cpVenus[L194]

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. iBE4max YE1 NG CLYBL

    Plasmid

    #174570

    Purpose

    doxycycline inducible BE4max YE1 NG expression for human CLYBL locus knock-in

    Depositor

    Mathew Garnett

    Article

    Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

    Insert

    BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet ON

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Mito-PyronicSF/pCMV-myc-mito

    Plasmid

    #124813

    Purpose

    Highly resposive GFP-based pyruvate nanosensor to explore mitochondrial metabolism.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Insert

    PyronicSF
    

    Use

    Tags

    Mitochondrial Sequence Signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDuet TDP-43 Q331K

    Plasmid

    #27465

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Coexpression

    Tags

    Expression

    Bacterial

    Mutation

    Glutamine 331 mutated to Lysine (Q331K)

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGW_GPHN.FingR-YFP

    Plasmid

    #46298

    Depositor

    Don Arnold

    Article

    Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

    Insert

    GPHN.FingR (GPHN Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFB1.HMBP.PrS.EZH2

    Plasmid

    #125161

    Purpose

    Expresses human EZH2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Baculovirus expression vector

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9

    Plasmid

    #175525

    Purpose

    Co-expression of HaloTag10 (nuclear) and 9 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag10 and Tomm20-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag10 = HaloTag7-Q165W and HaloTag9 = HaloTag…

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28-hTDG

    Plasmid

    #70758

    Purpose

    bacterial expression of human TDG

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    TDG (TDG Human)
    

    Use

    Tags

    6-HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lac operon

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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