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We narrowed to 4,764 results for: IRES

Showing: 1401 - 1420 of 4764 results

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pLVX-EF1alpha-nCoV2019-N-meGFP-IRES-Puro

Plasmid

#185451

Purpose

Expresses SARS-CoV-2 nucleocapsid protein with a c-terminal monomeric EGFP in mammalian cells

Depositor

Ahmet Yildiz

Article

Jack et al PLoS Biol. 2021 Oct 11;19(10):e3001425.

Insert

Nucleocapsid (N SARS-CoV-2)

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER

Plasmid

#113898

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-IRES-Puro GFP-3xFLAG

Plasmid

#201636

Purpose

Over-expression of 3xFLAG-tagged GFP

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

GFP

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFP

Plasmid

#113888

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic EmGFP

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-C-Myc-DDK-IRES-Puro HEXIM1

Plasmid

#200726

Purpose

Lentiviral expression of Flag-Tagged human HEXIM1

Depositor

Claudio Alarcón

Insert

HEXIM1 (HEXIM1 Human)

Use

Lentiviral

Tags

Myc-DDK from backbone plasmid

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pUBC-Pfv-Sapphire-IRES-H2B-mCherry

Plasmid

#203648

Purpose

Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Use

Lentiviral

Tags

Sapphire-IRES-H2B-mCherry

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJRH-1328 LV EF1a-CD20 IRES-EGFP

Plasmid

#201918

Purpose

2nd generation lentiviral transfer plasmid for engineering cells to express human CD20

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

CD20

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine

Plasmid

#50455

Purpose

Double floxed Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promoter

Depositor

Bryan Roth

Has Service

AAV8

Insert

hM4D(Gi)-IRES-mCitrine (CHRM4 Human)

Use

AAV

Tags

HA

Expression

Mutation

See supplemental documents for DREADD mutations

Promoter

human Synapsin 1

Available since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2 LRP6-APEX2-Ires-eGFP-PAC

Plasmid

#180142

Purpose

used to biotinylate targets that are recruited near the receptor during Wnt signaling at different time periods

Depositor

Edward De Robertis

Article

Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

Insert

Human LRP6 (LRP6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-RAB3C-IRES-Puromycin

Plasmid

#134857

Purpose

Expresses EGFP-tagged synaptic vesicle marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Ras-related protein 3C

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/TMPRSS2 (variant 1).IRES-neo.WPRE (MT130)

Plasmid

#145843

Purpose

Lentiviral vector genome to ectopically express human TMPRSS2 variant 1

Depositor

Caroline Goujon

Article

Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

Insert

human TMPRSS2 variant 1 (TMPRSS2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

G8V

Promoter

SFFV

Available since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

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pJRH-1365 LV EF1a-CD28 IRES-EGFP

Plasmid

#201921

Purpose

2nd generation lentiviral transfer plasmid for engineering cells to express human CD28

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

CD28 (CD28 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-TfR20-SNAP-IRES-Puro

Plasmid

#171018

Purpose

Lentiviral expression of a transferrin receptor-SNAP-tag fusion in mammalian cells

Depositor

Angus Johnston

Article

FitzGerald et al Nat Commun. 2020 Sep 8;11(1):4482. doi:

Insert

Transferrin receptor (Human) - SNAP-tag (TFRC Human)

Use

Lentiviral

Tags

SNAPtag and TfR

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-Calr-KDEL-IRES-Puromycin

Plasmid

#134664

Purpose

Expresses EGFP-tagged endoplasmic reticulum marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

calreticulin (CALR Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 18-413

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pIR-CMV-SCN2A-Variant-4-IRES-mScarlet

Plasmid

#209410

Purpose

Expression of wild-type neonatal isoform of human SCN2A.

Depositor

Al George

Article

Thompson et al bioRxiv. 2023 Feb 23:2023.02.23.529757.

Insert

SCN2A Variant 4, stabilized (SCN2A Human)

Use

Tags

Expression

Mammalian

Mutation

IVS intron inserted after bp 4551 of the SCN2A OR…

Promoter

CMV

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-C-Myc-DDK-IRES-Puro HNRNPA1

Plasmid

#200727

Purpose

Lentiviral expression of Flag-Tagged human HNRNPA1

Depositor

Claudio Alarcón

Insert

HNRNPA1 (HNRNPA1 Human)

Use

Lentiviral

Tags

Myc-DDK from backbone plasmid

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W

Plasmid

#32520

Depositor

Mustafa Sahin

Article

Nie et al Nat Neurosci. 2010 Feb;13(2):163-72. Epu

Insert

Constitutively active Rheb (S16H) (Rheb Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

S16H to make constitutively active

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1alpha-IRES-EGFP-3XFlag-hCEP164

Plasmid

#122966

Purpose

Expresses 3XFlag-tagged human CEP164 in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Insert

CEP164 (CEP164 Human)

Use

Lentiviral

Tags

3XFlag

Expression

Mutation

Promoter

EF1 alpha

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB-rDA3m-IRES-EGFP-CAAX

Plasmid

#208696

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m and a membrane-localized EGFP in mammalian cells

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA3m

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pSFFV_Gal4LOV[SK22]-VP64-IRES-mCherry (pLZA140)

Plasmid

#203907

Purpose

Encodes LightsOut (AsLOV2(408-543) inserted in Gal4 SK22) transcription factor in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

Gal4LOV(SK22)-VP64-IRES-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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