PurposeCRISPR donor plasmid to tag human transcription factor ESR1 with GFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||112337||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameESR1 homology arms flanking EGFP-IRES-Neo cassette
SpeciesH. sapiens (human)
Entrez GeneESR1 (a.k.a. ER, ESR, ESRA, ESTRR, Era, NR3A1)
- Cloning method Gibson Cloning
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer M13pUC-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Cloning source: K562. Use with plasmid pESR1.1.0-gDNA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pESR1-donor was a gift from Kevin White (Addgene plasmid # 112337 ; http://n2t.net/addgene:112337 ; RRID:Addgene_112337)