We narrowed to 5,091 results for: codon optimized

1. pLenti-VQR-PGK-Puro

   Plasmid

   #110855

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQR
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D1135V, R1335Q, T1337R

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-VRER-PGK-Puro

   Plasmid

   #110856

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRER
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-TO-oNGN2

   Plasmid

   #198397

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
   puroR-T2A-mycNLS-mTagBFP2
   rtTA3G
   

   Use

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…

   Promoter

   CAG, EF1a, and TRE3G

   Available since

   April 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas9_GFP

   Plasmid

   #44719

   Purpose

   Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Cas9-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFC334

   Plasmid

   #87846

   Purpose

   AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

   Inserts

   Cas9
   argB
   yA specific sgRNA
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   A. nidulans gpdA promoter with Hammerhead ribozym…

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDIV298

   Plasmid

   #204957

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7 and pyrG selection marker

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   pyrG
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and native pro…

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTRdeltaGFPmirGE-DroshaT1.5.5

   Plasmid

   #61084

   Purpose

   pENTR plasmid containing a mutated GFP followed by 3 miRNA haipins: recommended as backbone.

   Depositor

   Patrick Salmon

   Article

   Myburgh et al Mol Ther Nucleic Acids. 2014 Oct 28;3:e2

   Insert

   deltaGFP
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDIV302

   Plasmid

   #204973

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7 and nat1 resistance marker

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   nat1
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and Aspergillu…

   Available since

   Nov. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDIV299

   Plasmid

   #204967

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7 and argB selection marker

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   argB
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and native pro…

   Available since

   Nov. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFGC-pcoCas9

    Plasmid

    #52256

    Purpose

    A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA

    Depositor

    Jen Sheen

    Insert

    35SPPDK:pcoCas9:NOS cassette
    

    Use

    CRISPR; Plant expression

    Tags

    Expression

    Mutation

    Promoter

    constitutive 35SPPDK promoter

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. human Alpha-synuclein

    Plasmid

    #51437

    Purpose

    human codon optimized Alpha-synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Alpha-synuclein (SNCA Human)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pSMH1741

    Plasmid

    #160930

    Purpose

    Expression of soluble Sc octamer (H3)

    Depositor

    Stephen Harrison

    Inserts

    HHF1
    HTA1
    HTB1
    HHT1
    

    Use

    Tags

    6His-TEV, N-6His-TEV, N-terminal 6His-TEV, and no…

    Expression

    Mutation

    codon-optimized for E. coli -- see provided seque…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSMH1742

    Plasmid

    #160929

    Purpose

    Expression of soluble Sc octamer (Cse4)

    Depositor

    Stephen Harrison

    Inserts

    HHF1
    HTA1
    HTB1
    Cse4
    

    Use

    Tags

    6His-TEV, N-terminal 6His-TEV, and none

    Expression

    Mutation

    codon-optimized for E. coli -- see provided seque…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmCellFree_KA1_SARS-COV-2 _NSP1

    Plasmid

    #169376

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP1 with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _NSP1
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Expression

    Mutation

    Codon optimized for Saccharomyces cerevisiae

    Promoter

    T7- CMV/CAG

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGB3 omega1 Tnos:BASTA:Pnos - P35S:hCas9:Tnos (GB3469)

    Plasmid

    #160647

    Purpose

    module containing the human codon optimized Cas9 and the BASTA selection marker

    Depositor

    Diego Orzaez

    Insert

    BASTA / Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos, P35S

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHH0103 KIBRA WW domain #1

    Plasmid

    #104229

    Purpose

    Bacterial expression of WW domain #1 from KIBRA

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    KIBRA WW domain #1 (WWOX Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. MYLK4

    Plasmid

    #39175

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MYLK4 (MYLK4 Human)
    

    Use

    Baculovirus expression

    Tags

    His Tev

    Expression

    Mutation

    N371S, codon-optimized

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pET-29b(+)_notufA-CmEndoCOM_3C-CBD-His

    Plasmid

    #218198

    Purpose

    Bacterial expression of a His-tagged Chitin Binding fusion of Cordyceps militaris EndoCOM

    Depositor

    Florence Pojer

    Insert

    EndoCOM
    

    Use

    Tags

    3C-CBD-His

    Expression

    Bacterial

    Mutation

    codon optimized Ecoli, residues 19-315

    Promoter

    T7

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. m.3047-Left TALE-G1397-N-dead-mCherry

    Plasmid

    #211339

    Purpose

    Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection

    Depositor

    Monkol Lek

    Insert

    m.3047-Left TALE-G1397-N-dead-mCherry
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. m.3075-Left TALE-G1397-N-dead-mCherry

    Plasmid

    #211340

    Purpose

    Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection

    Depositor

    Monkol Lek

    Insert

    m.3075-Left TALE-G1397-N-dead-mCherry
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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