We narrowed to 28,993 results for: OMP;

1. Str-KDEL_ST-SBP-EGFP

   Plasmid

   #65264

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
   

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET29b-AK1-His

   Plasmid

   #124135

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Adenylate kinase (AK1 Chicken)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH_Str-KDEL_ManII-SBP-EGFP

   Plasmid

   #65258

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin-KDEL and truncated Mannosidase II
   

   Use

   Lentiviral

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available Since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Str-KDEL_ST-SBP-mCherry

   Plasmid

   #65265

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
   

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available Since

   June 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACYC-TtCas6-4xcrRNA4.5

   Plasmid

   #127764

   Purpose

   For expression of T. thermophilus Csm complex in E. coli (crRNA)

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

   Inserts

   Synthetic CRISPR array containing four identical T. thermophilus spacers
   Cas6A
   

   Expression

   Bacterial

   Mutation

   Codon optimized for expression in E. coli

   Promoter

   T7

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. SAP145

   Plasmid

   #11239

   Depositor

   Robin Reed

   Article

   Gozani et al Genes Dev. 1996 Jan 15. 10(2):233-43.

   Insert

   SAP145 (SF3B2 Human)
   

   Expression

   Bacterial

   Mutation

   First 7 amino acids are missing compared to NP_00…

   Available Since

   Jan. 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. Str-KDEL_ManII-SBP-mCherry

   Plasmid

   #65253

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin-KDEL and truncated Mannosidase II
   

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSL0527 (pDonor)

   Plasmid

   #130634

   Purpose

   Encodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchCAST donor DNA (Mini-Tn)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH_Str-KDEL_ManII-SBP-mCherry

   Plasmid

   #65259

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin-KDEL and truncated Mannosidase II
   

   Use

   Lentiviral

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Str-KDEL_ManII-SBP-EGFP

    Plasmid

    #65252

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin-KDEL and truncated Mannosidase II
    

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSL0915 (CRISPR with target 3)

    Plasmid

    #130643

    Purpose

    Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA that targets lacZ.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    CRISPR(target-3)
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. ST-SBP-mCherry_puromycin

    Plasmid

    #65271

    Purpose

    ST reporter only ( no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    truncated sialyltransferase (ST6GAL1 Human)
    

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAdSh.U6.gRNAGFP

    Plasmid

    #58255

    Purpose

    U6 promoter-driven single guide RNA complementary to eGFP

    Depositor

    Manuel Goncalves

    Article

    Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

    Insert

    U6.gRNAGFP cassette
    

    Use

    Adenoviral and CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Ii-Str_ST-SBP-mCherry

    Plasmid

    #65269

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ii-Streptavidin and truncated sialyltransferase (ST6GAL1 Human)
    

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available Since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Str-KDEL_ST-SBP-tagBFP

    Plasmid

    #65266

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
    

    Tags

    Streptavidin Binding Protein (SBP) and tagBFP

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available Since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSL0426 (CRISPR with spc3)

    Plasmid

    #130642

    Purpose

    Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    CRISPR(spacer-3)
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAdSh.U6.gRNAS1

    Plasmid

    #58252

    Purpose

    U6 promoter-driven single guide RNA complementary to the human AAVS1 locus

    Depositor

    Manuel Goncalves

    Article

    Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

    Insert

    U6.gRNAS1 cassette
    

    Use

    Adenoviral and CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ST-SBP-EGFP_puromycin

    Plasmid

    #65270

    Purpose

    ST reporter only ( no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    truncated sialyltransferase (ST6GAL1 Human)
    

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Str-KDEL_ManII-SBP-tagBFP

    Plasmid

    #65254

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin-KDEL and truncated Mannosidase II
    

    Tags

    Streptavidin Binding Protein (SBP) and tagBFP

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available Since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SMT205

    Plasmid

    #134817

    Purpose

    SMT201 with mutated RBS for DHFR, in vivo assays

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7 and TET

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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