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Multiplex genome editing of human pluripotent stem cells using Cpf1
Haiting Ma, Rudolf Jaenisch
bioRxiv
Article

Plasmids from Article

ID Plasmid Purpose  
194715CMV:AsCpf1-2A-GFP-U6-sgRNA-cloning vectorBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that can be cloned with BbsI sites
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194716CMV:AsCpf1-2A-GFP-U6-AAVS1-sgBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hAAVS1 locus
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194717CMV:AsCpf1-2A-GFP-U6-GFP-sgBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target GFP
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194718CMV:AsCpf1-2A-GFP-U6-MAFB-sgBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hMAFB
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194719CMV:AsCpf1-2A-GFP-U6-INS-sgBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hINS
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194720pX458-MAFB-sgpx458 with guide RNA that target hMAFB
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194721pX458-AAVS1-sgpx458 with guide RNA that target hAAVS1
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194722CMV:AsCpf1-2A-GFP-U6-tdTomato-sgBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target tdTomato
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194723CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNAbased on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target hAAVS1
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194724CAGGS-AsCpf1-2A-GFP-U6-tdTomato-sgRNAbased on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target tdTomato
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194725CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNAbased on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFB
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194726CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vectorCAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector
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194727CAGGS-AsCpf1(D908A)-2A-GFP-U6-AAVS1-sgRNACAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector, with guide RNA array targeting hAAVS1.
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194728AAVS1-tdTomato targeting vector (compatible with CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA)AAVS1-tdTomato targeting vector, compartible with Cpf1
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Recombinant Antibodies from Article