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Plasmid#184108Purposecyclofen-inducible apoptosis activation and imaging (mCherry) in zebrafish permanent transgenicDepositorInsertmyr-DIAP1-nls'-mCherry-P2A-Casp9-ERT2
UseZebrafish transgenesis (blue eyes)TagsExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …Promoter4xUASAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
V21 CS2+ RFPbb
Plasmid#17112DepositorTypeEmpty backboneUseXenopus/mammalian/avian/zebrafishTagsRFPExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
V20 CS2+ RFPbe
Plasmid#17111DepositorTypeEmpty backboneUseXenopus/mammalian/avian/zebrafishTagsRFPExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCS2-LAMP1-EGFP
Plasmid#169003PurposeExpress rat LAMP1-EGFP in zebrafishDepositorInsertrat LAMP1-EGFP
UseUnspecifiedTagsEGFPExpressionMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
actc1b-Lifeact-mKate2
Plasmid#109493PurposeMuscle specific zebrafish expression of mKate2 fused to the actin binding lifeact sequenceDepositorInsertactc1b-Lifeact-mKate2
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+cPLA2-mKate2
Plasmid#162068PurposeUsed to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseAvian, xenopus, zebrafishTagsmKate2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-ndufs2 guide
Plasmid#107601PurposeAn entry vector with U6a and U6c promoter driving ndufs2 guide RNAs expressionDepositorInsertndufs2 guide RNA sequences
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
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p3E-MCS
Plasmid#75174PurposeMultiple cloning site for insertion of gene of interest for C-terminal fusions.DepositorInsertMultiple Cloning Site
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2-LAMP1-mRFP
Plasmid#169004PurposeExpress rat LAMP1-mRFP in zebrafishDepositorInsertrat LAMP1-mRFP
UseUnspecifiedTagsmRFPExpressionMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Mito-mRFP
Plasmid#169001PurposeExpress Mito (Su9)-mRFP in zebrafishDepositorInsertMito (Su9)-mRFP (encoding a mitochondrial matrix targeting sequence of subunit 9 of the V0 ATPase Su9 of N. crassa)
UseUnspecifiedTagsmRFPExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVk7-33G1-Flag
Plasmid#58950Purposebackbone for mouse recombinant antibody with added C-terminal FLAG tagDepositorTypeEmpty backboneUseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME-mTagBFP
Plasmid#75148PurposeTagBFP (blue) fluorophore for use with 5’ promoters.DepositorInsertmTagBFP
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tol2-krt4-GFP
Plasmid#97151Purposelabel apical epithelial cells in zebrafishDepositorInsertAcGFP
UseZebrafishTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-rac2 guide
Plasmid#107600PurposeAn entry vector with U6a and U6c promoter driving rac2 guide RNAs expressionDepositorInsertrac2 guide RNA sequences
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry
Plasmid#191500PurposeGROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eyeDepositorInsertCaSR-EGFP-SV40
UseCreation of transgenic fish using tol2 transgenes…TagsExpressionMutationPromotermyo6bAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVk7-33G1-BioLHis
Plasmid#58949Purposebackbone for mouse recombinant antibody with added C-terminal biotinylation site, linker, 6His tagDepositorTypeEmpty backboneUseTags6His tag and biotinylation peptideExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pTol2pA2-mnx1:MLS-KillerRed
Plasmid#115515PurposePlasmid driving expression of membrane bound KillerRed fluorophore in zebrafish motor neuronsDepositorInsertMLS-KillerRed
UseGateway cloningTagsMLSExpressionMutationPromoter-3mnx1Available sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT3TS_Transposase
Plasmid#109768PurposeCodon optimised transposase sequence in an optimal vector for in vitro transcription. Contains the T3 promoter and synthetic polyA tract.DepositorInsertTol2 Transposase
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only