We narrowed to 3,602 results for: golden gate

1. pBH106

   Plasmid

   #215777

   Purpose

   NBU2 backbone with RbsR(YQR) and P(O1)_nanoluc

   Depositor

   Corey Wilson

   Article

   Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

   Inserts

   RbsR(YQR)
   Nanoluc
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Bt1311 and P(O1)

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBH116

   Plasmid

   #215779

   Purpose

   NBU2 backbone with anti-RbsR(YQR) and P(O1)_nanoluc

   Depositor

   Corey Wilson

   Article

   Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

   Inserts

   anti-RbsR(YQR)
   Nanoluc
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Bt1311 and P(O1)

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBH501

   Plasmid

   #215780

   Purpose

   NBU1 backbone with constitutive dCas9

   Depositor

   Corey Wilson

   Article

   Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

   Insert

   dCas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P1

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. GGDest1-Amp

   Plasmid

   #157649

   Purpose

   Golden Gate assembly of a pcDNA3.1+ or BioBrick RFC23 compatible fusion-protein ORF insert

   Depositor

   Karmella Haynes

   Article

   Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRDA_867

   Plasmid

   #231109

   Purpose

   A>G base editor

   Depositor

   David Root, John Doench

   Insert

   ABE8e-nCas9-SpG-HA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICSL4723-P1

   Plasmid

   #191786

   Purpose

   Level P Golden gate accepter

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRDB_270

   Plasmid

   #231111

   Purpose

   C>T base editor

   Depositor

   David Root, John Doench

   Insert

   TadA-CDd-nCas9-SpG-UGI-HA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLSL.R.Ly

   Plasmid

   #227697

   Purpose

   Reengineered geminiviral replicon vector

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. DVC_p15A_AE

   Plasmid

   #217587

   Purpose

   Destination vector with chloramphenicol resistance and p15A origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning.

   Depositor

   Wei Huang

   Article

   Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DVC_p15A_EF

    Plasmid

    #217589

    Purpose

    Destination vector with chloramphenicol resistance and p15A origin of replication. Carries E-F CIDAR MoClo overhangs for level 1 construct cloning.

    Depositor

    Wei Huang

    Article

    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. DVK_p15A_AF

    Plasmid

    #217583

    Purpose

    Destination vector with kanamycin resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.

    Depositor

    Wei Huang

    Article

    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. DVA_p15A_AF

    Plasmid

    #217604

    Purpose

    Destination vector with ampicillin resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 0 or 2 construct cloning.

    Depositor

    Wei Huang

    Article

    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET22b-TEVP-cleavableELP

    Plasmid

    #219402

    Purpose

    Tobacco etch virus protease-responsive elastin-like polypeptide (ELP)

    Depositor

    Danielle Mai

    Article

    Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

    Insert

    TEVP-cleavableELP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGGD003

    Plasmid

    #48835

    Purpose

    Provides linker:mCherry as GreenGate C-terminal tag module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    Linker-mCherry
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYY62-P1int-TP901-GFPdropout

    Plasmid

    #235773

    Purpose

    Plasmid used for constructing donor DNA for TP901-compatible p1 integration. This plasmid is a sfGFP dropout plasmid where sfGFP can be replaced by GOIs using Golden Gate Assembly.

    Depositor

    Chang Liu

    Article

    Pisera et al bioRxiv [Preprint]. 2024 Nov 30:2024.11.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBTK1051

    Plasmid

    #209541

    Purpose

    BTK Type 5 homology arm for Golden Gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Lariviere et al bioRxiv 2023.09.19.558440

    Insert

    Recombination into the S. alvi wkB2 genome within the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pGGC025

    Plasmid

    #48830

    Purpose

    Provides 3xGFP as GreenGate coding sequence module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    3xGFP
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGGF000

    Plasmid

    #48861

    Purpose

    Empty entry vector for creating GreenGate plant resistance cassette modules.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Type

    Empty backbone

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJet1.2 sfGFP

    Plasmid

    #117123

    Purpose

    C-terminal GFP tag

    Depositor

    Stephan Gruber

    Article

    Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

    Insert

    Superfolder GFP
    

    Use

    Golden gate donor vector for gene targeting in ba…

    Tags

    sfGFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGGA000

    Plasmid

    #48856

    Purpose

    Empty entry vector for creating GreenGate promoter modules.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Type

    Empty backbone

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge