We narrowed to 6,509 results for: golden gate
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Plasmid#121007PurposeMoClo golden gate assembly CD part for mScarlet-I-LAA (fast-folding mScarlet variant, codon-optimized for E. col, with LAA degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).DepositorInsertmScarlet-I codon optimized with LAA tag
UseSynthetic BiologyTagsLAA degradation tag for ssrAAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C70m
Plasmid#120982PurposeMoClo golden gate assembly CD part for RpaR-TYQEVV (Adam Meyer's improved RpaR activator selected for dynamic range and low cross-sensitivity using methods from https://doi.org/10.1101/285866,).DepositorInsertRpaR Adam Meyer
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P61m
Plasmid#120953PurposeMoClo golden gate assembly AB part for pTac (Adam Meyer's improved pTac promoter selected for dynamic range and low cross-sensitivity; unpublished, using strategy from https://doi.org/10.1101/285866).DepositorInsertTac promoter Adam Meyer
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
UC16m
Plasmid#121038PurposeMoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.DepositorInsertgQi Cas9 gRNA UC part
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
UC17m
Plasmid#121039PurposeMoClo golden gate assembly DE part for gV1 gRNA (guide RNA for S. pyogenes Cas9; sequence from doi: [10.15252/msb.20145735]). Please see Supplemental Documents for annotated Genbank file.DepositorInsertgV1 Cas9 gRNA UC part
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
UCT1m
Plasmid#121041PurposeMoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.DepositorInsertUCT-part guide RNA (Stanley Qi sequence)
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
EMMA toolkit
Plasmid kit#1000000119PurposeCollection of Golden Gate vectors and standardized genetic parts for the construction mammalian expression plasmids.DepositorApplicationCloning and Synthetic BiologyVector typeMammalian ExpressionCloning typeGolden GateAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEXY (pDN137)
Plasmid#72656Purposegolden gate entry vector for mCherry-LEXY tagging of proteins (no constitutive NLS)DepositorTypeEmpty backboneUseGolden gate entry vectorTagsmCherry-LEXYExpressionMammalianPromoterCMV-FAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pME_GGWDest+
Plasmid#49321PurposeDestination vector for Golden Gate assembly; middle entry vector for Gateway cloningDepositorTypeEmpty backboneUseGateway CloningAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGW_SM_BamHI
Plasmid#49315PurposeSubcloning vector for Golden Gate-based site-directed mutagenesis; BamHI and KpnI to release the insertDepositorTypeEmpty backboneUseCloning vectorAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
p3E_GGWDest-
Plasmid#49316PurposeDestination vector for Golden Gate assembly; 3' entry vector for Gateway cloningDepositorTypeEmpty backboneUseGateway CloningAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
p5E_GGWDest-
Plasmid#49318PurposeDestination vector for Golden Gate assembly; 5' entry vector for Gateway cloningDepositorTypeEmpty backboneUseGateway CloningAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGEV_+1_Linker
Plasmid#49280PurposeGolden Gate entry vector at position 1,small linker insert, plus 5' ATGDepositorTypeEmpty backboneUseCloning vectorAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGEV_3'_Linker
Plasmid#49289PurposeGolden Gate entry vector at position 3, small linker insert, final entry vector for the assemblyDepositorTypeEmpty backboneUseCloning vectorAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGEV_5'_Linker
Plasmid#49291PurposeGolden Gate entry vector at position 5, small linker insert, final entry vector for the assemblyDepositorTypeEmpty backboneUseCloning vectorAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGEV_-1_XcmI-LacZ
Plasmid#49296PurposeGolden Gate entry vector at position 1, no 5' ATG, LacZDepositorTypeEmpty backboneUseCloning vectorAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGGEV_-1_FRT_+1_OA-
Plasmid#49310PurposeGolden Gate entry vector: Position 1, FRT siteDepositorInsertFRT forward
UseCloning vectorAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGEV_3_FRT_+1_OA-
Plasmid#49311PurposeGolden Gate entry vector: Position 3, FRT siteDepositorInsertFRT forward
UseCloning vectorAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGEV_2_Linker
Plasmid#49282PurposeGolden Gate entry vector at position 2, small linker insertDepositorTypeEmpty backboneUseCloning vectorAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGEV_4_Linker
Plasmid#49284PurposeGolden Gate entry vector at position 4, small linker insertDepositorTypeEmpty backboneUseCloning vectorAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by