We narrowed to 3,816 results for: golden gate

1. pBH106

   Plasmid

   #215777

   Purpose

   NBU2 backbone with RbsR(YQR) and P(O1)_nanoluc

   Depositor

   Corey Wilson

   Article

   Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

   Inserts

   RbsR(YQR)
   Nanoluc
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Bt1311 and P(O1)

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBH116

   Plasmid

   #215779

   Purpose

   NBU2 backbone with anti-RbsR(YQR) and P(O1)_nanoluc

   Depositor

   Corey Wilson

   Article

   Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

   Inserts

   anti-RbsR(YQR)
   Nanoluc
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Bt1311 and P(O1)

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBH501

   Plasmid

   #215780

   Purpose

   NBU1 backbone with constitutive dCas9

   Depositor

   Corey Wilson

   Article

   Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

   Insert

   dCas9
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   P1

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GGDest1-Amp

   Plasmid

   #157649

   Purpose

   Golden Gate assembly of a pcDNA3.1+ or BioBrick RFC23 compatible fusion-protein ORF insert

   Depositor

   Karmella Haynes

   Article

   Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Promoter

   none

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJet1.2 sfGFP

   Plasmid

   #117123

   Purpose

   C-terminal GFP tag

   Depositor

   Stephan Gruber

   Article

   Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

   Insert

   Superfolder GFP
   

   Use

   Golden gate donor vector for gene targeting in ba…

   Tags

   sfGFP

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pICSL60004

   Plasmid

   #117519

   Purpose

   rbcS-E9 terminator Golden Gate Level 0

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BsaI:GCTT:E9t:CGCT:BsaI
   

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGGD005

   Plasmid

   #48853

   Purpose

   Provides linker:BFP as GreenGate C-terminal tag module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   Linker:BFP
   

   Use

   Golden gate compatible cloning vector

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLSL.R.Ly

   Plasmid

   #227697

   Purpose

   Reengineered geminiviral replicon vector

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSC142

   Plasmid

   #122835

   Purpose

   SUC2 promoter with GreenGate A-B flanking sequences

   Depositor

   Markus Schmid

   Article

   Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

   Insert

   SUC2 promoter
   

   Use

   Golden gate compatible cloning vector

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSR1154

    Plasmid

    #221653

    Purpose

    Tetracycline sensor v3 for Staphylococcus aureus

    Depositor

    Lauren Andrews

    Article

    Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

    Inserts

    sfGFP cassette
    TetR cassette
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PXyl-TetO and PZwf

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain

    Plasmid

    #228663

    Purpose

    Bacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Zika virus portion of NS2B protease coexpressed with NS3 protease
    

    Tags

    His-GST-TEV

    Expression

    Bacterial

    Available Since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pAttBF3Hyg

    Plasmid

    #244885

    Purpose

    Hyg resistant attB phiC31 recombination-mediation insertion (RMI) vector for C. elegans

    Depositor

    Michael Nonet

    Article

    Beck et al MicroPubl Biol. 2025 Sep 2;2025:10.17912

    Type

    Empty backbone

    Use

    Phic31 recombinase mediated insertion vector

    Expression

    Worm

    Promoter

    none

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRDA_867

    Plasmid

    #231109

    Purpose

    A>G base editor

    Depositor

    David Root, John Doench

    Insert

    ABE8e-nCas9-SpG-HA
    

    Use

    CRISPR and Lentiviral

    Mutation

    D10A

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGGA008

    Plasmid

    #48817

    Purpose

    Provides the alcA promoter as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    alcA promoter
    

    Use

    Golden gate compatible cloning vector

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGGC011

    Plasmid

    #48825

    Purpose

    Provides alcR as GreenGate coding sequence module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    alcR
    

    Use

    Golden gate compatible cloning vector

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAIP4_2PPN

    Plasmid

    #234190

    Purpose

    Heterologous protein expression of peptidyl-prolyl cis-trans isomerase FKBP1A (PPIase FKBP1A) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2PPN (FKBP1A Human)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pRDB_270

    Plasmid

    #231111

    Purpose

    C>T base editor

    Depositor

    David Root, John Doench

    Insert

    TadA-CDd-nCas9-SpG-UGI-HA
    

    Use

    CRISPR and Lentiviral

    Mutation

    D10A

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. DVC_pBR322_AF

    Plasmid

    #217598

    Purpose

    Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.

    Depositor

    Wei Huang

    Article

    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. DVC_p15A_AF

    Plasmid

    #217588

    Purpose

    Destination vector with chloramphenicol resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.

    Depositor

    Wei Huang

    Article

    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSF18

    Plasmid

    #222593

    Purpose

    BB2 ZWF1 tet-on overexpression cassette

    Depositor

    Matthias Steiger

    Article

    Matthias et al PLOS One

    Insert

    ZWF1
    

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert

    Promoter

    tet-on

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge