Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 4,698 results for: pcr

Showing: 1621 - 1640 of 4698 results

  1. pGS1021

    Plasmid
    #59575
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    Twin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
    Use
    E. coli cloning vector
    Tags
    Twin-Strep tag
    Expression
    Mutation
    Promoter
    None
    Available since
    Aug. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    59575-plasmid-map-sequence-id-92981

    Enlarge

  6. pGS1022

    Plasmid
    #59576
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    Twin-Strep tag optimized for PCR amplification followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    Twin-Strep tag
    Expression
    Mutation
    Promoter
    None
    Available since
    Aug. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    59576-plasmid-map-sequence-id-92983

    Enlarge

  9. pDONR221-kcnj11

    Plasmid
    #173139
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Article
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj11 (kcnj11 Zebrafish)
    Use
    Pcr cloning vector
    Tags
    Expression
    Mutation
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173139-plasmid-map-sequence-id-343178

    Enlarge

  10. pBP-ORF

    Plasmid
    #72949
    Purpose
    Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 0 - ORFs
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72949-plasmid-map-sequence-id-140106

    Enlarge

  16. pNDGG039

    Plasmid
    #231339
    Purpose
    Terminator; BEVA2.0 T15m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 T15m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    231339-plasmid-map-sequence-id-472145

    Enlarge

  17. pNDGG038

    Plasmid
    #231338
    Purpose
    Terminator; BEVA2.0 T12m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 T12m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    231338-plasmid-map-sequence-id-472130

    Enlarge

  18. pNDGG037

    Plasmid
    #231337
    Purpose
    Terminator; BEVA2.0 T2m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 T2m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    231337-plasmid-map-sequence-id-472127

    Enlarge

  19. pTE5423_p3WJdB-glcO60

    Plasmid
    #221194
    Purpose
    DNA template of GlcR sensor with glcO60 operator: T7 promoter-glcO60-3WJdB reporter-T7 terminator linear DNA template (PCR amplified with oSB0021+22) is used in the IVT sensor system
    Depositor
    Tobias Erb
    Article
    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0
    Insert
    T7 promoter-glcO60-3WJdB
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter with downstream glcO60 operator
    Available since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221194-plasmid-map-sequence-id-441878

    Enlarge

Showing: 1621 - 1640 of 4698 results