Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 3,611 results for: golden gate

Showing: 1681 - 1700 of 3611 results

  1. EC71174

    Plasmid
    #154067
    Purpose
    Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Inserts
    HS Cre recombinase
    Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
    HYGROMYCIN Resistance Cassette
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Tags
    Expression
    Bacterial and Plant
    Mutation
    The Arabidopsis thaliana U5 small nuclear ribonuc…
    Promoter
    35S promoter, Hordeum vulgare HSP 17 promoter use…
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    154067-plasmid-map-sequence-id-313091

    Enlarge

  2. PB-hEF1A-MG-U6-sgRNA

    Plasmid
    #175505
    Purpose
    All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1a, U6
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175505-plasmid-map-sequence-id-345098

    Enlarge

  3. GreenGate

    Plasmid Kit
    #1000000036
    Purpose
    Golden Gate cloning system for rapid assembly of plant transformation constructs; Uses 6 types of pre-cloned modules: promoter, N- and C-terminal tags, coding sequence, terminator, resistance cassette
    Depositor
    Jan Lohmann
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. G555

    Plasmid
    #216483
    Purpose
    Expression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.
    Depositor
    Christophe Danelon
    Insert
    plsB, plsC, cdsA, pssA
    Use
    Cell-free expression
    Tags
    Expression
    Mutation
    Promoter
    T7 for each gene
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    216483-plasmid-map-sequence-id-430343

    Enlarge

  18. pGGZ003

    Plasmid
    #48869
    Purpose
    Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.
    Depositor
    Jan Lohmann
    Article
    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:
    Type
    Empty backbone
    Use
    Golden gate compatible plant transformation vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    48869-plasmid-map-sequence-id-71306

    Enlarge

  19. pGGZ001

    Plasmid
    #48868
    Purpose
    Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA right border.
    Depositor
    Jan Lohmann
    Article
    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:
    Type
    Empty backbone
    Use
    Golden gate compatible plant transformation vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    48868-plasmid-map-sequence-id-71304

    Enlarge

  20. pBTK1050

    Plasmid
    #209540
    Purpose
    BTK Type 1 homology arm for Golden Gate assembly
    Depositor
    Jeffrey Barrick
    Article
    Lariviere et al bioRxiv 2023.09.19.558440
    Insert
    Upstream homology arm for recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    209540-plasmid-map-sequence-id-422358

    Enlarge

Showing: 1681 - 1700 of 3611 results