Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 4,795 results for: pcr

Clear keywords
Showing: 1701 - 1720 of 4795 results

  3. pJL263

    Plasmid
    #243699
    Purpose
    A piggyBac vector containing the NDUFB9 genomic sequence fused with a mNeonGreen2(11) sequence, modified with silent mutations to allow for PCR-based analysis
    Depositor
    Jonathan Weissman
    Article
    Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.
    Insert
    NDUFB9 (NDUFB9 Human)
    Tags
    mNeonGreen2(11)
    Expression
    Mammalian
    Available Since
    Nov. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    243699-plasmid-map-sequence-id-489320

    Enlarge

  4. pJL378

    Plasmid
    #243716
    Purpose
    A piggyBac vector containing the UQCRQ coding sequences, modified with silent mutations to allow for PCR-based analysis, along with endogenous promoter and UTRs.
    Depositor
    Jonathan Weissman
    Article
    Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.
    Insert
    UTRs and CDS of UQCRQ (UQCRQ Human)
    Expression
    Mammalian
    Promoter
    endogenous promoter
    Available Since
    Nov. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    243716-plasmid-map-sequence-id-489244

    Enlarge

  5. pAct-GST-H3

    Plasmid
    #182842
    Purpose
    A PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.
    Depositor
    Peter Harte
    Article
    Tie et al Development. 2009 Sep;136(18):3131-41. d
    Insert
    GST-histone H3 fusion
    Tags
    GST
    Expression
    Insect
    Promoter
    Actin5C
    Available Since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    182842-plasmid-map-sequence-id-360059

    Enlarge

  6. pENTR-D-kcnj13

    Plasmid
    #173143
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Article
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj13 (kcnj13 Zebrafish)
    Use
    Pcr cloning vector
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173143-plasmid-map-sequence-id-343318

    Enlarge

  7. pENTR-D-kcnj1b

    Plasmid
    #173126
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Article
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj1b (kcnj1b Zebrafish)
    Use
    Pcr cloning vector
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173126-plasmid-map-sequence-id-342841

    Enlarge

  18. pR26

    Plasmid
    #127372
    Purpose
    Allows for doxycycline-inducible gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.
    Depositor
    Lance Miller
    Article
    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Mouse Targeting
    Promoter
    tight TRE promoter
    Available Since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    127372-plasmid-map-sequence-id-247988

    Enlarge

  19. pSG424 ELKC(S383A)

    Plasmid
    #89334
    Purpose
    Elk1 cDNA encoding aa 307-428, aa383 mutated from ser-Ala, The PCR product was cut with BamH1 and Xba 1(365bp). pSG424 was cut with Bam H1 and Xba1. The Elk1 fragment was ligated into the BamH1
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.
    Insert
    ELK1 (ELK1 Human)
    Tags
    Gal 4
    Expression
    Mammalian
    Mutation
    encodes aa 307-428, changed serine 383 to alanine…
    Available Since
    April 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    89334-plasmid-map-sequence-id-176433

    Enlarge

  20. pR26-CMVconst

    Plasmid
    #127373
    Purpose
    Allows for constitutive gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.
    Depositor
    Lance Miller
    Article
    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Mouse Targeting
    Promoter
    CMV enhancer/promoter
    Available Since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    127373-plasmid-map-sequence-id-248011

    Enlarge

Showing: 1701 - 1720 of 4795 results