We narrowed to 3,795 results for: golden gate

1. pD(Ct)_3C-Venus-His8

   Plasmid

   #223889

   Purpose

   4G-cloning donor for C-terminal 3C-Venus-His8 tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   3C-Venus-His8
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pD(P+T)_CMVC

   Plasmid

   #223862

   Purpose

   4G-cloning donor for mammalian promoter and terminator, C-terminal tag added

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   CMV promoter and bGH polyA
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. DVA_pBR322_AH

   Plasmid

   #217606

   Purpose

   Destination vector with ampicillin resistance and pBR322 origin of replication. Carries A-H CIDAR MoClo overhangs for level 0 or 2 construct cloning.

   Depositor

   Wei Huang

   Article

   Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. DVK_p15A_FG

   Plasmid

   #217585

   Purpose

   Destination vector with kanamycin resistance and p15A origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.

   Depositor

   Wei Huang

   Article

   Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. DVK_pBR322_GH

   Plasmid

   #217596

   Purpose

   Destination vector with kanamycin resistance and pBR322 origin of replication. Carries G-H CIDAR MoClo overhangs for level 1 construct cloning.

   Depositor

   Wei Huang

   Article

   Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. DVK_p15A_GH

   Plasmid

   #217586

   Purpose

   Destination vector with kanamycin resistance and p15A origin of replication. Carries G-H CIDAR MoClo overhangs for level 1 construct cloning.

   Depositor

   Wei Huang

   Article

   Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. DVK_p15A_EF

   Plasmid

   #217584

   Purpose

   Destination vector with kanamycin resistance and p15A origin of replication. Carries E-F CIDAR MoClo overhangs for level 1 construct cloning.

   Depositor

   Wei Huang

   Article

   Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. DVA_p15A_AG

   Plasmid

   #217603

   Purpose

   Destination vector with ampicillin resistance and p15A origin of replication. Carries A-G CIDAR MoClo overhangs for level 0 or 2 construct cloning.

   Depositor

   Wei Huang

   Article

   Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. DVA_p15A_EH

   Plasmid

   #217605

   Purpose

   Destination vector with ampicillin resistance and p15A origin of replication. Carries E-H CIDAR MoClo overhangs for level 0 or 2 construct cloning.

   Depositor

   Wei Huang

   Article

   Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DVA_pBR322_AG

    Plasmid

    #217607

    Purpose

    Destination vector with ampicillin resistance and pBR322 origin of replication. Carries A-G CIDAR MoClo overhangs for level 0 or 2 construct cloning.

    Depositor

    Wei Huang

    Article

    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. DVC_pBR322_EF

    Plasmid

    #217599

    Purpose

    Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries E-F CIDAR MoClo overhangs for level 1 construct cloning.

    Depositor

    Wei Huang

    Article

    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. DVC_pBR322_FG

    Plasmid

    #217600

    Purpose

    Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.

    Depositor

    Wei Huang

    Article

    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. DVC_pBR322_GH

    Plasmid

    #217601

    Purpose

    Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries G-H CIDAR MoClo overhangs for level 1 construct cloning.

    Depositor

    Wei Huang

    Article

    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. DVC_pBR322_AE

    Plasmid

    #217597

    Purpose

    Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning.

    Depositor

    Wei Huang

    Article

    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. DVC_p15A_GH

    Plasmid

    #217591

    Purpose

    Destination vector with chloramphenicol resistance and p15A origin of replication. Carries G-H CIDAR MoClo overhangs for level 1 construct cloning.

    Depositor

    Wei Huang

    Article

    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. DVA_pBR322_AF

    Plasmid

    #217608

    Purpose

    Destination vector with ampicillin resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 0 or 2 construct cloning.

    Depositor

    Wei Huang

    Article

    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. DVA_pBR322_EH

    Plasmid

    #217609

    Purpose

    Destination vector with ampicillin resistance and pBR322 origin of replication. Carries E-H CIDAR MoClo overhangs for level 0 or 2 construct cloning.

    Depositor

    Wei Huang

    Article

    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBH502

    Plasmid

    #215781

    Purpose

    NBU2 backbone with LacI(YQR) and P(O1)_sgRNA targeting Nanoluc

    Depositor

    Corey Wilson

    Article

    Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

    Inserts

    LacI(YQR)
    sgRNA-ribozyme cassette
    Nanoluc
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Bt1311, P(O1), and cfxA

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET22b-Empty-cleavableELP

    Plasmid

    #219401

    Purpose

    Insert protease recognition site with BsaI to make custom protease-responsive elastin-like polypeptide (ELP)

    Depositor

    Danielle Mai

    Article

    Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

    Insert

    Empty-cleavableELP
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET22b-FXa-cleavableELP

    Plasmid

    #219403

    Purpose

    Factor Xa-responsive elastin-like polypeptide (ELP)

    Depositor

    Danielle Mai

    Article

    Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

    Insert

    FXa-cleavableELP
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge