We narrowed to 3,550 results for: golden gate

1. MSGV Rh Acceptor

   Plasmid

   #64271

   Purpose

   Retroviral golden gate cloning vector for expression of functional Rhesus Macaque T-Cell Receptor genes

   Depositor

   David Ott

   Article

   Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOGG014

   Plasmid

   #113988

   Purpose

   Vector construction module. Endlinker/terminator module to circularize plasmid following Part 4

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   Endlinker/terminator Part 4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOGG015

   Plasmid

   #113989

   Purpose

   Vector construction module. Endlinker/terminator module to circularize plasmid following Part 5

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   Endlinker/terminator Part 5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOGG013

   Plasmid

   #113987

   Purpose

   Vector construction module. Endlinker/terminator module to circularize plasmid following Part 3

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   Endlinker/terminator Part 3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOGG016

   Plasmid

   #113990

   Purpose

   Vector construction module. Endlinker/terminator module to circularize plasmid following Part 6

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   Endlinker/terminator Part 6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOGG202

   Plasmid

   #115503

   Purpose

   Clone of a level 1 unit which encodes IPTG-inducible sfGFP

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   pL1M-F1-plac pOGG031, sfGFP pOGG037, T-pharma pOGG003
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOGG203

   Plasmid

   #115504

   Purpose

   Clone of a level 1 unit which encodes a constitutively-expressed mCherry

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   pL1M-F2-pNeo pOGG001, mCherry EC15071, T-pharma(pOGG003
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pL1P6 ZmUbiP:GRF-GIF:NosT

   Plasmid

   #198049

   Purpose

   MoClo Golden Gate Level 1 Position 6. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

   Depositor

   Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing

   Insert

   ZmUbiP::TaGRF4-GIF1::NosT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Zea mays (maize) ubiquitin promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLM433

   Plasmid

   #217293

   Purpose

   For creating CyanoTag vectors by Golden Gate cloning (BspQI version)

   Depositor

   Luke Mackinder

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   mNeonGreen - 3x FLAG

   Expression

   Mutation

   Promoter

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMC-Dest-BsaI

    Plasmid

    #200550

    Purpose

    Mini circle destination vector with BsaI sites for Golden Gate Assembly

    Depositor

    Junsu Kang

    Type

    Empty backbone

    Use

    Minicircle cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. L0I80_Bxb1mut(noBsaI)_GG1-GG4

    Plasmid

    #219715

    Purpose

    Level 0 Bxb1 integrase with mutated BsaI sites, GG1 and GG4 BsaI sites for golden gate assembly.

    Depositor

    Jennifer Nemhauser

    Insert

    GG1-Bxb1(noBsaI0)-GG4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOPS0379

    Plasmid

    #115510

    Purpose

    Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing sfGFP

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Rlv3841pNifH(pOGG082) sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOPS0254

    Plasmid

    #115506

    Purpose

    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing celB

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Rlv3841pNifH pOGG082, celB pOGG050, T-pharma pOGG003 assembled in pOGG026
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOPS0377

    Plasmid

    #115509

    Purpose

    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing sfGFP

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Constitutive promoter pNeo pOGG001

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSGV Hu Acceptor PGK-NGFR

    Plasmid

    #64270

    Purpose

    Retroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes with NGFR

    Depositor

    David Ott

    Article

    Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOPS0750

    Plasmid

    #115511

    Purpose

    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing gusA.

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Constitutive promoter pNeo pOGG001

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOPS0314

    Plasmid

    #115507

    Purpose

    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing celB

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    celB pOGG050, T-pharma pOGG003 assembled in pOGG026
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Constitutive promoter pNeo pOGG001

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOPS0253

    Plasmid

    #115505

    Purpose

    Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing gusA.

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Rlv3841pNifH pOGG082, gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSGV Rh Acceptor PGK-NGFR

    Plasmid

    #64272

    Purpose

    Retroviral golden gate cloning vector for expression of functional Rhesus T-Cell Receptor genes with NGFR

    Depositor

    David Ott

    Article

    Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGGD_MiniTurboID-YFP

    Plasmid

    #222434

    Purpose

    Golden Gate / Green Gate module for adding MiniTurboID and YFP as C-terminus of target protein.

    Depositor

    Dominique Bergmann

    Article

    Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

    Insert

    MiniTurboID (BirA mutant)
    

    Use

    Golden gate / green gate compatible cloning vector

    Tags

    Linker and YFP

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge