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pBBK55 pHomL-RPL18Bp-PIL1-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179039

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PIL1-Link-Neon(gfp), a marker for the eisosomes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PIL1-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK53 pHomL-RPL18Bp-Neon(gfp)-Link-SFT2-SSA1t-HomR (AmpR)

Plasmid

#179037

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-SFT2, a marker for the late Golgi

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-SFT2

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK52 pHomL-RPL18Bp-Neon(gfp)-Link-SED5-SSA1t-HomR (AmpR)

Plasmid

#179036

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-SED5, a marker for the early Golgi

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-SED5

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK50 pHomL-RPL18Bp-ARC35-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179034

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of ARC35-Link-Neon(gfp), a marker for the actin cytoskeleton

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-ARC35-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK48 pHomL-RPL18Bp-SPC42-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179032

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of SPC42-Link-Neon(gfp), a marker for the spindle pole

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-SPC42-Link-Neon(gfp)

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK47 pHomL-RPL18Bp-SRM1-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179031

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of SRM1-Link-Neon(gfp), a marker for the nucleus

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-SRM1-Link-Neon(gfp)

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK46 pHomL-RPL18Bp-RPC19-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179030

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of RPC19-Link-Neon(gfp), a marker for the nucleolus

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-RPC19-Link-Neon(gfp)

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK14 pCAS-Tyr-[gRNA: 4=XII-5] (SplitKanR, AmpR)

Plasmid

#178998

Purpose

Sp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK13 pCAS-Tyr-[gRNA: 3=XII-2] (SplitKanR, AmpR)

Plasmid

#178997

Purpose

Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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ystagRFP-T2A-mTq2 pdrf1-GW

Plasmid

#168072

Purpose

Produces equimolar expression of ystagRFP and mCherry for use in yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026; doi: https://doi.o

Insert

ystagRFP-T2A-mTq2

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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ymTq2-mAvicFP1 pDRF1-GW

Plasmid

#168075

Purpose

FRET fusion between ymTq2 and mAvicFP1 for use in yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026; doi: https://doi.o

Insert

ymTq2-mAvicFP1

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-T2A-mCherry pDRF1-GW

Plasmid

#168060

Purpose

Produces equimolar expression of mGreenLantern and mCherry for use in yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026; doi: https://doi.o

Insert

mGreenLantern-T2A-mCherry

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMJB1-RPL1B-Cl

Plasmid

#160430

Purpose

Expresses "cre-less" yeast RPL1B tagged with mCherry under the GAL1 promoter

Depositor

Gary Stormo

Article

Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

Insert

RPL1B

Tags

mCherry

Expression

Yeast

Mutation

Removed introns, shuffled codons to vary nucleoti…

Promoter

S. cerevisiae GAL1

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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BS-Trp1

Plasmid

#69196

Purpose

complementation of yeast auxotrophic marker TRP1. Although this is the plasmid described in the paper, the depositor notes that it has a low integration efficiency and Plasmid 69503 should be used.

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

TRP1

Expression

Yeast

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pEMY01AB-PRO

Plasmid

#127714

Purpose

Integrative yeast promoter characterization plasmid. Clone in with BpiI. Has ChrXV homology upstream and an mVenus fragment downstream.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pEMY01CD-TER

Plasmid

#127751

Purpose

Integrative yeast terminator characterization plasmid. Clone in with BpiI. Has mVenus fragment upstream and a KanMX fragment downstream.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pHCMM1

Plasmid

#167452

Purpose

Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

TEF1

Availability

Academic Institutions and Nonprofits only

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pHCMM2

Plasmid

#167453

Purpose

Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

PSEC63

Availability

Academic Institutions and Nonprofits only

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pAG423GPD-ccdB

Plasmid

#14150

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Expression

Yeast

Available Since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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pCTcon2

Plasmid

#41843

Depositor

Dane Wittrup

Article

Chao et al Nat Protoc. 2006;1(2):755-68.

Insert

CD20

Tags

HA and c-myc

Expression

Yeast

Available Since

Jan. 23, 2013

Availability

Academic Institutions and Nonprofits only

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