We narrowed to 16,257 results for: form

1. GFP-BioID-FAM21

   Plasmid

   #121046

   Purpose

   Expresses GFP tagged BioID and human FAM21C in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

   Inserts

   BioID
   FAM21C (FAM21C Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   R118G (highly promiscuous form)

   Promoter

   CMV

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-UBC-ERT2-iCre-ERT2

   Plasmid

   #178060

   Purpose

   Conditionally active form of Cre recombinase (activated in response to tamoxifen) under UBC promoter.

   Depositor

   Edward Boyden

   Article

   Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ERT2-iCre-ERT2
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human ubiquitin C (UBC) promoter

   Available Since

   Oct. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQFlag-USP7 CS puroR

   Plasmid

   #46752

   Purpose

   Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP7

   Depositor

   Goedele Maertens, Gordon Peters

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP7 (USP7 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   C223S--catalytically inactive

   Promoter

   CMV

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDest-Sumo

   Plasmid

   #106980

   Purpose

   LR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tag

   Depositor

   Han Choe

   Article

   Nguyen et al Sci Rep. 2017 Nov 23;7(1):16139. doi: 10

   Type

   Empty backbone

   Tags

   His8-Sumo

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-cofilin-S3A

   Plasmid

   #78296

   Purpose

   Mammalian expression of mouse cofilin S3A (nonphosphorylatable form) fused to EGFP

   Depositor

   Michael Frotscher

   Article

   Chai et al Development. 2016 Mar 15;143(6):1029-40.

   Insert

   cofilin 1 (Cfl1 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S3A

   Promoter

   CAG

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQHA-USP7 CS puroR

   Plasmid

   #46754

   Purpose

   Retroviral vector that expresses catalytically inactive form of HA-tagged human USP7

   Depositor

   Goedele Maertens, Gordon Peters

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP7 (USP7 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C223S--catalytically inactive

   Promoter

   CMV

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSKC12 - DelQ

   Plasmid

   #110493

   Purpose

   Monitor the expression of Luciferase driven by a mutated NRAS 5'-UTR.

   Depositor

   Shankar Balasubramanian

   Article

   Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi

   Insert

   NRAS 5'- DelQ (bp 30-254) (NRAS Human)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Mutation

   Deletion of the first 29 base pairs of the NRAS 5…

   Promoter

   T7

   Available Since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSCALPs-HA-NFAT1 (4-460)-GFP

   Plasmid

   #88879

   Purpose

   Lentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFP

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

   Insert

   HA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)
   

   Use

   Lentiviral

   Tags

   GFP fusion protein and HA tag

   Expression

   Mammalian

   Mutation

   amino acids 4-460 only; L78P & L287P *see bel…

   Promoter

   SFFV (spleen focus forming virus)

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQE80L-SpyCatcher-ELP-GFP

   Plasmid

   #69835

   Purpose

   Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

   Insert

   SpyCatcher-ELP-GFP
   

   Tags

   6x His Tag, GFP, and TEV Tag

   Expression

   Bacterial

   Promoter

   T5 promoter/lac operator element

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-BLM DM

    Plasmid

    #80071

    Purpose

    Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations

    Depositor

    Nathan Ellis

    Article

    Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6

    Insert

    BLM (BLM Human)
    

    Tags

    GFP-BLM

    Expression

    Mammalian

    Mutation

    K317R (AAA>AGA) and K331R (AAA>AGA)

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO5d.SFFV.eGFP-miR30n

    Plasmid

    #90333

    Purpose

    miR30 based shMir knockdown

    Depositor

    Dirk Heckl

    Article

    Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10

    Inserts

    eGFP
    na
    SFFV
    miR30n
    

    Use

    Lentiviral

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. beta catenin binding mutant of alpha catenin sensor

    Plasmid

    #71711

    Purpose

    This form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctions

    Depositor

    Deborah Leckband

    Article

    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

    Insert

    alpha-catenin Δβ-cat mutant FRET Sensor
    

    Tags

    ECFP and YPET

    Expression

    Mammalian

    Mutation

    Deleted amino acid residues 48- 163

    Promoter

    CMV

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. VBS mutant of alpha catenin sensor

    Plasmid

    #71710

    Purpose

    This form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.

    Depositor

    Deborah Leckband

    Article

    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

    Insert

    alpha-catenin ΔVBS FRET Sensor
    

    Tags

    ECFP and YPET

    Expression

    Mammalian

    Mutation

    Deleted amino acid residues 316-405 in α -catenin…

    Promoter

    CMV

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO5d.SFFV.dTomato-miR30n

    Plasmid

    #90334

    Purpose

    miR30 based shMir knockdown

    Depositor

    Dirk Heckl

    Article

    Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10

    Inserts

    dTomato
    na
    SFFV
    miR30n
    

    Use

    Lentiviral

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. gp64-mVEGF-C-HisTag-pENTR

    Plasmid

    #64065

    Purpose

    Plasmid encodes mature form of murine VEGF-C preceded by baculoviral gp64 secretion signal; entry vector to Gateway cloning system.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Protein Expr Purif. 2015 Mar 7. pii: S10

    Insert

    vascular endothelial growth factor C (Vegfc Mouse)
    

    Tags

    His-tag

    Expression

    Insect

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28-MRPP3-His

    Plasmid

    #67866

    Purpose

    bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tag

    Depositor

    Walter Rossmanith

    Article

    Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

    Insert

    MRPP3 (KIAA0391 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino acid 46 of recombinant MRPP3 is preceded by…

    Promoter

    T7

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28-MRPP3-myc-His

    Plasmid

    #67864

    Purpose

    bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. myc-His tag

    Depositor

    Walter Rossmanith

    Article

    Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

    Insert

    MRPP3 (KIAA0391 Human)
    

    Tags

    His and myc

    Expression

    Bacterial

    Mutation

    Amino acid 46 of recombinant MRPP3 is preceded by…

    Promoter

    T7

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Delta DED FLIP-L D/A

    Plasmid

    #13791

    Depositor

    Guy Salvesen

    Article

    Boatright et al Biochem J. 2004 Sep 1. 382(Pt 2):651-7.

    Insert

    DELTA DED FLIP LONG (CFLAR Human)
    

    Tags

    8 x His tag

    Expression

    Bacterial

    Mutation

    Delta DED removes first 186 residues Asp to Ala m…

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. HBM-mVEGF-C-HisTag-pENTR

    Plasmid

    #64066

    Purpose

    Plasmid encodes mature form of murine VEGF-C preceded by honey bee melittin secretion signal; entry vector to Gateway cloning system.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Protein Expr Purif. 2015 Mar 7. pii: S10

    Insert

    vascular endothelial growth factor C (Vegfc Mouse)
    

    Tags

    His-tag

    Expression

    Insect

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. USPQ-HTT-TALEN1

    Plasmid

    #92243

    Purpose

    TALEN targeting upstream of HTT gene (KKR), forms obligate heterodimer with USPQ-HTT-TALEN2

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    TALEN
    

    Use

    TALEN

    Promoter

    CMV

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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