We narrowed to 3,769 results for: golden gate

1. pJet1.2 kanR cassette

   Plasmid

   #117122

   Purpose

   Kanamycin resistance cassette

   Depositor

   Stephan Gruber

   Article

   Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

   Insert

   Kanamycin resistance cassette for Bacillus subtilis selection
   

   Use

   Golden gate donor vector for gene targeting in ba…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBR_attB(bxb)_ccdB_lox_v2

   Plasmid

   #198040

   Purpose

   Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. G555

   Plasmid

   #216483

   Purpose

   Expression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.

   Depositor

   Christophe Danelon

   Insert

   plsB, plsC, cdsA, pssA
   

   Use

   Cell-free expression

   Tags

   Expression

   Mutation

   Promoter

   T7 for each gene

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBTK1050

   Plasmid

   #209540

   Purpose

   BTK Type 1 homology arm for Golden Gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Lariviere et al bioRxiv 2023.09.19.558440

   Insert

   Upstream homology arm for recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pGGZ001

   Plasmid

   #48868

   Purpose

   Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA right border.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Type

   Empty backbone

   Use

   Golden gate compatible plant transformation vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGGZ003

   Plasmid

   #48869

   Purpose

   Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Type

   Empty backbone

   Use

   Golden gate compatible plant transformation vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJet1.2 cat cassette

   Plasmid

   #117119

   Purpose

   Chloramphenicol resistance cassette

   Depositor

   Stephan Gruber

   Article

   Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

   Insert

   Chloramphenicol resistance cassette for Bacillus subtilis selection
   

   Use

   Golden gate donor vector for gene targeting in ba…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJet1.2 specR cassette

   Plasmid

   #117120

   Purpose

   Spectinomycin resistance cassette

   Depositor

   Stephan Gruber

   Article

   Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

   Insert

   Spectinomycin resistance cassette for Bacillus subtilis selection
   

   Use

   Golden gate donor vector for gene targeting in ba…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EC10161

   Plasmid

   #154060

   Purpose

   Level 0.5 Golden Gate backbone, containing loxP sites.

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   N/A

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. GG-dest

    Plasmid

    #69538

    Purpose

    Destination plasmid for gRNA concatenation Golden Gate cloning

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGGB003

    Plasmid

    #48821

    Purpose

    Provides a default dummy as GreenGate N-terminal tag module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    B-Dummy
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. BCJJ125

    Plasmid

    #117515

    Purpose

    Cas9_3 (with intron) Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:AATG:Cas9_3(intron):GCTT:BsaI
    

    Use

    CRISPR

    Tags

    2xFLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGGF005

    Plasmid

    #48846

    Purpose

    Provides a hygromycin resistance cassette as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    pUBQ10::HygromycinR::tOCS
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGGF007

    Plasmid

    #48847

    Purpose

    Provides a kanamycin resistance cassette as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    pNOS::KanamycinR::tNOS
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGGD002

    Plasmid

    #48834

    Purpose

    Provides a default dummy as GreenGate C-terminal tag module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    D-Dummy
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGGA006

    Plasmid

    #48816

    Purpose

    Provides the UBQ10 promoter as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    UBQ10 promoter
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMOD_C'0000a

    Plasmid

    #127226

    Purpose

    Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMOD_D0000a

    Plasmid

    #127230

    Purpose

    Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGGY001

    Plasmid

    #48866

    Purpose

    Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA right border.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Type

    Empty backbone

    Use

    Golden gate compatible plant transformation vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGGE001

    Plasmid

    #48839

    Purpose

    Provides the rbcS terminator as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    rbcS terminator
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge