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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,839 results for: pcr

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Showing: 1921 - 1940 of 4839 results

  1. pJUNB-donor

    Plasmid
    #112313
    Purpose
    CRISPR donor plasmid to tag human transcription factor JUNB with GFP
    Depositor
    Kevin White
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    JUNB homology arms flanking EGFP-IRES-Neo cassette (JUNB Human)
    Use
    CRISPR
    Mutation
    hg38:19:12792992:C>G, hg38:19:12793018:T>G,…
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112313-plasmid-map-sequence-id-228468

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  2. pZC14 (pFA6a-PSc-6xGly-V5-HphMX)

    Plasmid
    #44079
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44079-plasmid-map-sequence-id-61697

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  3. pZC03 (pFA6a-TEV-6xGly-V5-His3MX)

    Plasmid
    #44072
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, His3…
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44072-plasmid-map-sequence-id-61692

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  4. pZC11 (pFA6a-TEV-6xGly-V5-KanMX)

    Plasmid
    #44097
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Available since
    April 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    44097-plasmid-map-sequence-id-61694

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  5. pZC04 (pFA6a-PSc-6xGly-PrtA-His3MX)

    Plasmid
    #44066
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, His3…
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44066-plasmid-map-sequence-id-61687

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  11. HRH3-Tango

    Plasmid
    #66402
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HRH3 (HRH3 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. XA106+pRCPam

    Bacterial strain
    #195858
    Purpose
    E. coli nonsense suppressor strain (leuX) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows magenta when CP is induced with rhamnose.
    Depositor
    Gregory Phillips
    Publication
    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)
    Bacterial resistance
    Chloramphenicol
    Species
    Acropora millepora
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only

  13. XA103+pRCPam

    Bacterial strain
    #195857
    Purpose
    E. coli nonsense suppressor strain (tyrT) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. No color when grown with or without rhamnose.
    Depositor
    Gregory Phillips
    Publication
    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)
    Bacterial resistance
    Chloramphenicol
    Species
    Acropora millepora
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only

  14. XA101+pRCPam

    Bacterial strain
    #195856
    Purpose
    E. coli nonsense suppressor strain (serU) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows salmon when CP is induced with rhamnose.
    Depositor
    Gregory Phillips
    Publication
    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)
    Bacterial resistance
    Chloramphenicol
    Species
    Acropora millepora
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only

  15. XA92+pRCPam

    Bacterial strain
    #195855
    Purpose
    E. coli nonsense suppressor strain (glnX) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows purple when CP is induced with rhamnose.
    Depositor
    Gregory Phillips
    Publication
    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)
    Bacterial resistance
    Chloramphenicol
    Species
    Acropora millepora
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only

  17. GPR18-Tango

    Plasmid
    #66340
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPR18 (GPR18 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  18. CHRM5-Tango

    Plasmid
    #66252
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CHRM5 (CHRM5 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. GPR75-Tango

    Plasmid
    #66372
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPR75 (GPR75 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    66372-plasmid-map-sequence-id-456479

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  20. GPR64-Tango

    Plasmid
    #66369
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPR64 (ADGRG2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66369-plasmid-map-sequence-id-314165

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Showing: 1921 - 1940 of 4839 results