We narrowed to 3,913 results for: golden gate
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Plasmid#220248Purposereplicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expressionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only
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pIVT-mGL
Plasmid#232768PurposeIVT template for making modRNA encoding mGreenLanternDepositorInsertmGreenLantern
UseIn vitro transcriptionAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIVT-Bxb1
Plasmid#232746PurposeIVT template for making modRNA encoding Bxb1DepositorInsertBxb1
UseIn vitro transcriptionTagsHA and SV40 NLSAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
MbCas12a_N1153_GPG_GR2
Plasmid#231578PurposeEncodes a cortisol inducible variant of MbCas12aDepositorInsertMbCas12a with a GR2 insertion behind N1153
UseCRISPRExpressionMammalianAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination
Plasmid#40132PurposeDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).DepositorTypeEmpty backboneTagsFokI nuclease (ELD)ExpressionMammalianPromoterCAGAvailable SinceAug. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination
Plasmid#40131PurposeDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).DepositorTypeEmpty backboneTagsFokI nuclease (KKR)ExpressionMammalianPromoterCAGAvailable SinceAug. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYPQ146
Plasmid#69298PurposeGolden Gate recipient and Gateway entry vector; assembly of 6 gRNAsDepositorTypeEmpty backboneUseCRISPRAvailable SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
MET-HYGRO
Plasmid#218113PurposeIntegration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T7-TALEN(Sangamo)-Destination
Plasmid#37184PurposeDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.DepositorTypeEmpty backboneTagsFokI nucleaseExpressionMammalianPromoterCAGAvailable SinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJJB1577
Plasmid#218619PurposeExpress Pex5, Pex2, Pex17, and Pex10DepositorInsertPex5
ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW111117
Plasmid#222460PurposeLevel 1 acceptor, Position 3 containing CBS4-CMV1::LoxP-mCherry-stop-LacZ::nosT.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionPlantMutationnoPromoterCBS4-CMV1 (Cucumber mosaic virus)Available SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW110035
Plasmid#222444PurposeLevel 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry-stop.DepositorInsertLoxP-mCherry-stop
UseSynthetic BiologyExpressionPlantMutationnoAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRO117
Plasmid#233823PurposeMoClo-YTK; type3; Reporter; mNeonGreenDepositorInsertmNeonGreen
ExpressionYeastAvailable SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPiggyBacATCC LacZ GCTT loxAttP
Plasmid#173496PurposepiggyBac insect transgenesis destination vector for Golden Gate Cloning assembliesDepositorTypeEmpty backboneExpressionInsectAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIVT-PuroR
Plasmid#232753PurposeIVT template for making modRNA encoding the Puromycin resistance geneDepositorInsertPuroR
UseIn vitro transcriptionAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSBLego
Plasmid#219967PurposeThe pSBLego vector enables sybodies for the Legobody method with a PelB signal for periplasmic expression and a "SLEHHHHHH" motif for purification and Legobody Fab binding.DepositorTypeEmpty backboneTagsSLEHHHHHH and pelB signal sequenceExpressionBacterialPromoterpBADAvailable SinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-gRNA_BbsI_CAM
Plasmid#183222PurposeLbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate AssemblyDepositorInsertLbCas12a guide RNA containing BbsI sites in spacer sequence
UseCRISPRExpressionBacterialAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29-neoLux1.2
Plasmid#229952PurposeBacterial expression for de novo luciferase, neoLux1.2DepositorInsertneoLux1.2
TagsHis-tagExpressionBacterialPromoterT7Available SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCHW111101
Plasmid#222452PurposeLevel 1, Position 7 containing CBS4-miniDFR::Cre::NOS.DepositorInsertCBS4-miniDFR::Cre::NOS
UseSynthetic BiologyExpressionPlantMutationnoAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ145
Plasmid#69297PurposeGolden Gate recipient and Gateway entry vector; assembly of 5 gRNAsDepositorTypeEmpty backboneUseCRISPRAvailable SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only