We narrowed to 7,048 results for: golden gate

1. pPD1151 pLink 1

   Plasmid

   #139239

   Purpose

   Vector that holds the linker for circuits with 1 TUs

   Depositor

   Joshua Leonard

   Insert

   Linker1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. C9m

   Plasmid

   #120958

   Purpose

   MoClo golden gate assembly CD part for deCFP (cyan fluorescent protein) - Please see Supplemental Documents for annotated Genbank file

   Depositor

   Richard Murray

   Insert

   deCFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. C76m

   Plasmid

   #120988

   Purpose

   MoClo golden gate assembly CD part for KanR (kanamycin resistance protein). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   KanR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PACE_AP_GIII_Titrate

   Plasmid

   #242140

   Purpose

   PSP-LacO Cassette; AP Prop Circuit for AraC phage-assisted evolution

   Depositor

   Dominik Niopek

   Insert

   Gene III
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBD_PYR1_156-F1

   Plasmid

   #241286

   Purpose

   Backbone for PYR1 sensor library assembly from oligopools; stop codon removed; PYR1 sensor library assembly from oligopools;

   Depositor

   Sean Cutler

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAIP4_1EDG

   Plasmid

   #234144

   Purpose

   Heterologous protein expression of GUNA_RUMCH in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   1EDG
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. U4m

   Plasmid

   #121017

   Purpose

   MoClo golden gate assembly BC part for UTR1 (very strong RBS). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   UTR1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAV10.F3

   Plasmid

   #63199

   Purpose

   BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAV10.KH

   Plasmid

   #63208

   Purpose

   pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAV10.KN

    Plasmid

    #63209

    Purpose

    pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEN-X-hPGK-PuroR-Z

    Plasmid

    #218904

    Purpose

    golden gate cloning

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    hPGK-PuroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    246T>A and 398T>C

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. ku70 insUP+pPGI+NAT+tNOS+ku70 insD (SBE217)

    Plasmid

    #195049

    Purpose

    integration cassette for ku70 deletion and expression of NAT gene under pPGI for characterization

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    NAT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pPGI

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAV10.K5

    Plasmid

    #63210

    Purpose

    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAV10.K4

    Plasmid

    #63212

    Purpose

    pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. (K) tNOS (S)_T3 (SBE120)

    Plasmid

    #195010

    Purpose

    terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T3 position (K/S)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (K) tNOS (S)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. (H) tNOS (I) _T2 (SBE119)

    Plasmid

    #195006

    Purpose

    terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T2 position (H/I)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (H) tNOS (I)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. (B) HYG marker(R) (SBE264)

    Plasmid

    #195013

    Purpose

    hygromycin (HYG) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    pXYL+HYG+tGPD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pXYL

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. (E) tNOS (F) _T1 (SBE118)

    Plasmid

    #194997

    Purpose

    terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T1 position (E/F)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (E) tNOS (F)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. (B) BLE marker(R) (SBE262)

    Plasmid

    #195012

    Purpose

    bleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    pXYL+BLE+tGPD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pXYL

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAV113.loxP

    Plasmid

    #63186

    Purpose

    pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge