Addgene

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pmEos2-N1 GBP (GBP-mEos2)

Plasmid

#162876

Purpose

Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

GFP Binding Protein tagged with mEos2

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pmEos2-C1 PA-PLA1 (mEos2-PA-PLA1)

Plasmid

#162878

Purpose

Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with mEos2 to perform sptPALM

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)

Plasmid

#99229

Purpose

Mammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos2

Use

Tags

Lifeact

Expression

Mammalian

Mutation

A69T for primed conversion applications

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

Plasmid

#162877

Purpose

Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Insert consists of AA1-170

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCLC-mEos2

Plasmid

#38010

Depositor

Xiaowei Zhuang

Article

Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

Insert

Clathrin, light polypeptide (LCa) (Clta Mouse)

Use

Tags

mEos2

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSETa mEos2

Plasmid

#20341

Purpose

bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)

Depositor

Loren Looger

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

mEos-2

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
mEos2-C1

Plasmid

#54510

Purpose

Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Type

Empty backbone

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits
mEos2-N1

Plasmid

#54662

Purpose

Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Type

Empty backbone

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
mEos2-CENPA

Plasmid

#57359

Purpose

Localization: Nucleus, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CENPA (CENPA Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits
Paxillin_PSCFP2_ mEOS2

Plasmid

#114192

Purpose

studying focal adhesion proteins by superresolution microscopy (PALM) with dual labelling

Depositor

Aleksandra Radenovic

Insert

Paxillin (PXN Chicken)

Use

Tags

PS-CFP2 and mEos2

Expression

Mammalian

Mutation

Promoter

SV40

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGAMS-mEos2

Plasmid

#105128

Purpose

Expresses human FGAMS-mEos2 fusion in mammalian cells

Depositor

Stephen Benkovic

Article

French et al Science. 2016 Feb 12;351(6274):733-7. do

Insert

PFAS (PFAS Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
FLN-mEos2

Plasmid

#126807

Purpose

express FLN fusion

Depositor

David Knecht

Insert

filamin-mEos2

Use

Dictyostelium expression

Tags

mEos2

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAGmEos2-N1

Plasmid

#46372

Depositor

Suliana Manley

Article

Gunzenhauser et al Nano Lett. 2012 Sep 12;12(9):4705-10. do

Insert

Gag

Use

Tags

mEos2

Expression

Mammalian

Mutation

Rev independentHXB2 Gag sequence

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
mEos2-ER-5

Plasmid

#57373

Purpose

Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

ER (CALR Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits
mEos2-H1-10

Plasmid

#57383

Purpose

Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

H1

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
mEos2-Display-5

Plasmid

#54632

Purpose

Localization: Cytoplasm, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Type

Empty backbone

Use

Tags

mEos2-Display

Expression

Mammalian

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits
mEos2-Peroxisomes-2

Plasmid

#54750

Purpose

Localization: Peroxisomes, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Type

Empty backbone

Use

Tags

Peroximal Targeting Signal 1 (PTS1) and mEos2

Expression

Mammalian

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits
mEos2-Lifeact-7

Plasmid

#54809

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Type

Empty backbone

Use

Tags

mEos2-Lifeact

Expression

Mammalian

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
mEos2-Actin-7

Plasmid

#57339

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Actin (ACTB Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits
mEos2-Actin-C-18

Plasmid

#57340

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Actin (ACTB Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits