162 results

1. CRISPi Kit

   Plasmid Kit

   #1000000136

   Purpose

   CRISPi Kit contains elements for CRISPR/Cas9 mediated genome editing in yeast P. pastoris. Contains 7 different backbone (BB3) vectors with Cas9/ sgRNA expression cassettes with NTC, hphMX, or kanMX.

   Depositor

   Brigitte Gasser

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

2. GoldenPiCS Kit

   Plasmid Kit

   #1000000133

   Purpose

   GoldenPiCS: plasmid kit for engineering in P. pastoris (GoldenMOCS based). Overexpression of up to eight transcription units. 20 promoters, 10 terminators, 4 selection markers and 4 integration sites

   Depositor

   Brigitte Gasser

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

3. TRUPATH

   Plasmid Kit

   #1000000163

   Purpose

   A collection of 20 plasmids encoding alpha, beta, or gamma subunits of the heterotrimeric G protein complex for detection of heterotrimer dissociation. GPCR

   Depositor

   Bryan Roth

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

4. PRESTO-Tango Kit

   Plasmid Kit

   #1000000068

   Purpose

   Used to measure receptor activation for more than 300 G-protein coupled receptors (GPCRs) via a modified Tango beta-arrestin recruitment assay.

   Depositor

   Bryan Roth

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

5. MoClo Plant Parts III: Transformation & Genome Engineering Kit

   Plasmid Kit

   #1000000236

   Purpose

   The MoClo Plant Parts III: Transformation & Genome Engineering Kit contains 94 Level 0 parts, compatible with the Phytobrick/MoClo system for golden gate assembly of plant transformation constructs.

   Depositor

   Daniel Voytas

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

6. Multiple Lentiviral Expression (MuLE) system

   Plasmid Kit

   #1000000060

   Purpose

   Multiple Lentiviral Expression (MuLE): modular system used to construct complex lentiviruses for modification of mammalian cells with a single viral infection.

   Depositor

   Ian Frew

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

7. MoClo Tool Kit

   Plasmid Kit

   #1000000044

   Purpose

   Eukaryotic multigene construct assembly in 3 steps using modular cloning (MoClo); Has 95 empty standardized genetic modules: promoters, 5' UTRs, signal peptides, coding sequences, and terminators

   Depositor

   Sylvestre Marillonnet

   Available Since

   April 8, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

8. MoClo Yeast Toolkit

   Plasmid Kit

   #1000000061

   Purpose

   96 standardized parts used for bottom-up hierarchical assembly of single and multi-gene constructs for S. cerevisiae expression. Includes promoters, terminators, peptide tags, selection cassettes

   Depositor

   John Dueber

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

9. S. cerevisiae Advanced Gateway Destination Vectors

   Plasmid Kit

   #1000000011

   Purpose

   Yeast (S. cerevisiae) Gateway Destination Vectors for tagging and gene expression. Has variety of promoters, origin of replications, auxotrophic markers, and N- or C-terminal fusion proteins

   Depositor

   Susan Lindquist

   Available Since

   July 16, 2011

   Availability

   Academic Institutions and Nonprofits only

10. CIDAR MoClo Parts Kit

    Plasmid Kit

    #1000000059

    Purpose

    CIDAR Modular Cloning (MoClo) kit uses modular DNA parts and enhanced MoClo protocols to enable rapid one-pot, multipart assembly, combinatorial design, and expression tuning in E. coli

    Depositor

    Douglas Densmore

    Available Since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. biGBac Kit

    Plasmid Kit

    #1000000088

    Purpose

    Generation of baculoviral transfer vectors coding up to 25 protein complex subunits in two Gibson assembly steps. Multisubunit protein complexes can be generated in baculovirus-infected insect cells.

    Depositor

    Jan-Michael Peters

    Available Since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

12. CyanoGate Kit

    Plasmid Kit

    #1000000146

    Purpose

    The CyanoGate kit contains 96 parts and acceptor vectors for generating multi-part assemblies in either integrative (suicide) or self-replicating plasmid vectors for working in cyanobacteria.

    Depositor

    Alistair McCormick

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

13. MoClo Plant Parts Kit

    Plasmid Kit

    #1000000047

    Purpose

    Modular Cloning (MoClo) collection contains 95 standardized parts to enable Golden Gate construction of multi-gene constructs for plant transformation

    Depositor

    Nicola Patron, Sylvestre Marillonnet

    Available Since

    April 8, 2014

    Availability

    Academic Institutions and Nonprofits only

14. Multiplex CRISPR dCas9/FokI-dCas9 Accessory Pack

    Plasmid Kit

    #1000000062

    Purpose

    Accessory pack for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either dCas9 or dCas9-FokI fusion; Kit #1000000055 is required to use this kit

    Depositor

    Takashi Yamamoto

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

15. GreenGate

    Plasmid Kit

    #1000000036

    Purpose

    Golden Gate cloning system for rapid assembly of plant transformation constructs; Uses 6 types of pre-cloned modules: promoter, N- and C-terminal tags, coding sequence, terminator, resistance cassette

    Depositor

    Jan Lohmann

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

16. MoClo Yeast Secretion and Display (YSD) Toolkit

    Plasmid Kit

    #1000000240

    Purpose

    The YSD Toolkit is a collection of 34 plasmids that can be used in conjunction with the MoClo Yeast Toolkit (YTK) to optimize secretion and cell surface display of proteins of interest in yeast.

    Depositor

    Paul Young

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

17. Cultivarium POSSUM Toolkit

    Plasmid Kit

    #1000000234

    Purpose

    The POSSUM Toolkit is a Golden Gate genetic toolkit developed to help researchers identify functional plasmids and establish genetic tractability in non-model microbes.

    Depositor

    Nili Ostrov

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

18. ONE-GO biosensors

    Plasmid Kit

    #1000000224

    Purpose

    This kit consists of ten ONE vector G protein Optical (ONE-GO) biosensor plasmids for the expression of BRET-based biosensors of G proteins activated by G-protein-coupled receptors (GPCRs).

    Depositor

    Mikel Garcia-Marcos

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

19. Golden Gate TALEN and TAL effector kit 2.0

    Plasmid Kit

    #1000000024

    Purpose

    Used to efficiently assemble TALEN constructs with custom repeat arrays, containing anywhere between 12 and 31 repeats. Uses Golden Gate cloning method and has been validated in multiple organisms.

    Depositor

    Adam Bogdanove, Daniel Voytas

    Available Since

    Jan. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

20. Yarrowia lipolytica Golden Gate tool kit

    Plasmid Kit

    #1000000167

    Purpose

    Yarrowia lipolytica Golden Gate tool kit for integrative vector assembly of up to three transcription units in the oleaginous yeast Y. lipolytica.

    Depositor

    Tristan Rossignol

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only