161 results
-
Plasmid Kit#1000000226PurposeThis kit is used for expressing recombinant proteins with site-specific, non-hydrolyzable phosphoserine (nhpSer) in an engineered self-sufficient E. coli genetic code expansion (GCE) expression systemDepositorAvailable SinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only
-
Plasmids for Multiplexed Optical Sensors Arrayed in Islands of Cells (MOSAIC)
Plasmid Kit#1000000175PurposePlasmids and entry vectors for diverse fluorescent sensors of physiological parameters in cells.DepositorAvailable SinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-itis Bacterial Base Editor Activity
Plasmid Kit#1000000219PurposeThis collection contains all plasmids needed for preparation and execution of the "GFP-itis" bacterial base editing activity.DepositorAvailable SinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
TALE Toolbox
Plasmid Kit#1000000019PurposeProvides a complete set of plasmids for building your own TALE nucleases (TALENs) and transcription factors (TALE-TFs) for genome editing and transcription modulation applications.DepositorAvailable SinceDec. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
Yeast Secrete and Detect
Plasmid Kit#1000000166PurposeYeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.DepositorAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human Kinase Domain Constructs for Automated Bacterial Expression
Plasmid Kit#1000000094Purpose68 His-tagged human kinase catalytic domain constructs and the respective phosphatases that enhance their bacterial expression; optimized for automated bacterial expressionDepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
EasyClone 2.0 Yeast Toolkit
Plasmid Kit#1000000073PurposeIntegrative constructs for (over-) expression of (heterologous) genes in both laboratory and industrial yeast (S. cerevisiae) strains; Allow for selection in auxotrophic and prototrophic yeast strainsDepositorAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
Fragmid
Plasmid Kit#1000000237PurposeFragmid is a modular toolkit that leverages Golden Gate cloning to enable the rapid creation of CRISPR vectors compatible with various delivery approaches, enzymes, and mechanisms.DepositorAvailable SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Shen Lab DdCBE assembly kit
Plasmid Kit#1000000212PurposeThe DddA-derived cytosine base editor (DdCBE) assembly kit is a collection of 192 modules harboring repeat variable di-residue (RVD) arrays for any desired target.DepositorAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
C. elegans miniMos transgenesis kit
Plasmid Kit#1000000031Purpose29 plasmid kit contains reagents for generating transgenic nematodes, including C. elegans and C. briggsae, using miniMos transposon. Selection with unc-119 or NeoR, PuroR.DepositorAvailable SinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
RAS mutant clone collection
Plasmid Kit#1000000089PurposeWild-type and mutant HRAS, NRAS, KRAS4a and KRAS4b gene plasmids as Gateway entry clonesDepositorAvailable SinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Multiplex CRISPR dCas9/FokI-dCas9 Accessory Pack
Plasmid Kit#1000000062PurposeAccessory pack for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either dCas9 or dCas9-FokI fusion; Kit #1000000055 is required to use this kitDepositorAvailable SinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
EcoFlex MoClo Extension, Volume I
Plasmid Kit#1000000253PurposeUse the EcoFlex extension kit (volume one) to quickly and efficiently build a wide variety of gene expression constructs using the EcoFlex-standard MoClo system.DepositorAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
MoBiFC
Plasmid Kit#1000000222PurposeThe modular bimolecular fluorescence complementation (MoBiFC) toolkit allows for the analysis of protein-protein interactions in plants.DepositorAvailable SinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
openCIDAR MoClo Toolkit
Plasmid Kit#1000000227PurposeThis collection includes broad host range, pBBR1-based vectors compatible with the CIDAR MoClo cloning standard. These vectors replicate in many bacteria across Pseudomonadota.DepositorAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
EMMA toolkit
Plasmid Kit#1000000119PurposeUsed for the construction of a variety of mammalian expression vectors in a one-pot, one-step Golden Gate reaction; compatible with automated productionDepositorAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
UbiGate Components (set1)
Plasmid Kit#1000000144PurposeGenerate bacterial expression cassettes of up to six ORFs simultaneously; also contains ubiquitin, the E1 UBA1, an E2 collection, and a selection of E3s and their substrates for ubiquitination assaysDepositorAvailable SinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Minimal Antibiotic Resistance Platform (ARP)
Plasmid Kit#1000000143PurposeUsed for the general dereplication of antibiotics in natural product extracts. Plasmids constitutively express individual resistance genes that target the most commonly found antibiotics.DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
CRIMPkit
Plasmid Kit#1000000225PurposeThe CRISPR/Cas9 Insertional Mutagenesis Protocol (CRIMP) and toolkit (CRIMPkit) is a universal and highly efficient targeted insertional mutagenesis system designed for use in zebrafish.DepositorAvailable SinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tol2 Gateway compatible toolbox for the study of the nervous system and neurodegenerative disease
Plasmid Kit#1000000087PurposeTol2 Zebrafish toolkit: 4 promoters, 6 fluorophores, MultiSite Gateway (Invitrogen) compatible and cloning sites. Production transgenic zebrafish for neurodegenerative disordersDepositorAvailable SinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only