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155 results

Showing: 61 - 80 of 155 results

  1. Golden Gate TALEN and TAL effector kit 2.0

    Plasmid Kit
    #1000000024
    Purpose
    Used to efficiently assemble TALEN constructs with custom repeat arrays, containing anywhere between 12 and 31 repeats. Uses Golden Gate cloning method and has been validated in multiple organisms.
    Depositor
    Adam Bogdanove, Daniel Voytas
    Available since
    Jan. 8, 2013
    Availability
    Academic Institutions and Nonprofits only

  2. MoBiFC

    Plasmid Kit
    #1000000222
    Purpose
    The modular bimolecular fluorescence complementation (MoBiFC) toolkit allows for the analysis of protein-protein interactions in plants.
    Depositor
    Benjamin Field
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only

  3. UbiGate E2 activity screen (set2)

    Plasmid Kit
    #1000000145
    Purpose
    Contains bacterial constructs for E3 autoubiquitination assay, including expression cassettes containing ubiquitin and the E1 UBA1 in combination with all Arabidopsis thaliana E2s
    Depositor
    Marco Trujillo
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only

  5. UbiGate Components (set1)

    Plasmid Kit
    #1000000144
    Purpose
    Generate bacterial expression cassettes of up to six ORFs simultaneously; also contains ubiquitin, the E1 UBA1, an E2 collection, and a selection of E3s and their substrates for ubiquitination assays
    Depositor
    Marco Trujillo
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only

  6. CCSB-Broad Human Kinase ORF Collection

    Plasmid Kit
    #1000000014
    Purpose
    556 distinct human kinases and kinase-related protein ORFs in a pDONR-223 Entry Gateway vector. All clones are clonal isolates.
    Depositor
    David Root, William Hahn
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  7. MetClo DNA Assembly Kit

    Plasmid Kit
    #1000000153
    Purpose
    MetClo DNA Assembly Kit allows modular, hierarchical assembly of DNA fragments using IIS restriction enzyme. Includes 3 E.coli strains for BsaI, BpiI and LguI-based MetClo assembly.
    Depositor
    Christopher A. O'Callaghan
    Available since
    April 22, 2019
    Availability
    Academic Institutions and Nonprofits only

  8. EXPRESSYALI

    Plasmid Kit
    #1000000245
    Purpose
    The EXPRESSYALI Set is a complete kit for the introduction of genes into Yarrowia chromosomal loci. The kit includes six part plasmids, 12 marker-free integrative vectors, and 11 gRNA plasmids.
    Depositor
    Irina Borodina
    Available since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only

  9. POMBOX MoClo YTK

    Plasmid Kit
    #1000000251
    Purpose
    Use POMBOX for seamless and efficient assembly of single gene and multigene plasmids for genome integration in S. pombe.
    Depositor
    Tomáš Pluskal
    Available since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only

  10. Golden GATEway Cloning Kit

    Plasmid Kit
    #1000000054
    Purpose
    Combinatorial assembly method that combines Golden Gate (Type IIs endonucleases) cloning with Multisite Gateway (recombination) cloning to construct complex plasmids in an efficient and modular way
    Depositor
    Joachim Wittbrodt
    Available since
    Sept. 10, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. CRISPi Kit

    Plasmid Kit
    #1000000136
    Purpose
    CRISPi Kit contains elements for CRISPR/Cas9 mediated genome editing in yeast P. pastoris. Contains 7 different backbone (BB3) vectors with Cas9/ sgRNA expression cassettes with NTC, hphMX, or kanMX.
    Depositor
    Brigitte Gasser
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only

  12. EMMA toolkit

    Plasmid Kit
    #1000000119
    Purpose
    Used for the construction of a variety of mammalian expression vectors in a one-pot, one-step Golden Gate reaction; compatible with automated production
    Depositor
    Yizhi Cai
    Available since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. Multiple Lentiviral Expression (MuLE) system

    Plasmid Kit
    #1000000060
    Purpose
    Multiple Lentiviral Expression (MuLE): modular system used to construct complex lentiviruses for modification of mammalian cells with a single viral infection.
    Depositor
    Ian Frew
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. Endy/Shearwin pOSIP plasmid set

    Plasmid Kit
    #1000000035
    Purpose
    11 Plasmids for use in clonetegration: method for decreasing time and effort needed for integration of DNA into prokaryotic chromosomes. Facilitates cloning, expression of difficult genetic elements
    Depositor
    Drew Endy
    Available since
    July 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. Yeast Prototrophy Kit

    Plasmid Kit
    #1000000085
    Purpose
    Plasmids derived from the pHLUM mini-chromosome, designed to restore prototrophy (eg HIS3, LEU2, URA3, MET17 or LYS2 genes) in common yeast (S. cerevisiae) laboratory strains.
    Depositor
    Markus Ralser
    Available since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  16. S. cerevisiae Advanced Gateway Destination Vectors

    Plasmid Kit
    #1000000011
    Purpose
    Yeast (S. cerevisiae) Gateway Destination Vectors for tagging and gene expression. Has variety of promoters, origin of replications, auxotrophic markers, and N- or C-terminal fusion proteins
    Depositor
    Susan Lindquist
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  17. FusX TALEN assembly system

    Plasmid Kit
    #1000000063
    Purpose
    Golden Gate assembly of 15-16 RVD TALEN inserts in a single cloning step; designed to be used with high throughput robotics
    Depositor
    Stephen Ekker
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. CRISPR-Cas9 Gateway System for Physcomitrella patens

    Plasmid Kit
    #1000000151
    Purpose
    CRISPR-Cas9 Gateway System is a collection of 24 plasmids providing a modular, efficient CRISPR/Cas9 editing system for accurate and customizable repair in Physcomitrella patens.
    Depositor
    Magdalena Bezanilla
    Available since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only

  19. GoldenPiCS Kit

    Plasmid Kit
    #1000000133
    Purpose
    GoldenPiCS: plasmid kit for engineering in P. pastoris (GoldenMOCS based). Overexpression of up to eight transcription units. 20 promoters, 10 terminators, 4 selection markers and 4 integration sites
    Depositor
    Brigitte Gasser
    Available since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  20. pCri System

    Plasmid Kit
    #1000000058
    Purpose
    pCri System: plasmids for cytoplasmic and periplasmic/extracellular heterologous protein expression in Escherichia coli, Bacillus subtilis, and Pichia pastoris.
    Depositor
    F Xavier Gomis-Ruth
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 61 - 80 of 155 results