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166 results

Showing: 101 - 120 of 166 results

  1. EcoFlex MoClo Extension, Volume I

    Plasmid Kit
    #1000000253
    Purpose
    Plasmids to quickly and efficiently build a wide variety of gene expression constructs using the EcoFlex-standard MoClo system (Addgene #1000000080).
    Depositor
    Mikhail Shapiro
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only

  2. FNL Combinatorial Cloning Platform

    Plasmid Kit
    #1000000211
    Purpose
    A Frederick National Laboratory (FNL) combinatorial cloning set to generate many tagged expression clones in parallel for protein production, in vivo gene expression, and shRNA and CRISPR delivery.
    Depositor
    Dominic Esposito
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Bacterial Expression, Insect Expression, Mammalian Expression
    Cloning Type
    MultiSite Gateway
    Available Since
    Dec. 22, 2022
    Availability
    Academic Institutions and Nonprofits only

  3. Cancer Pathways ORFs Kit

    Plasmid Kit
    #1000000072
    Purpose
    Vectors containing wild-type and mutant ORFs for studying and screening oncogenic signaling pathways.
    Depositor
    David Sabatini, Kris Wood
    Application
    Gene Expression and Labeling
    Vector Type
    Lentiviral, Mammalian Expression
    Available Since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only

  4. SapTrap-SEC kit for C. elegans genome engineering

    Plasmid Kit
    #1000000109
    Purpose
    A collection of C. elegans plasmids used for building homologous repair templates that incorporate a self-excising drug selection cassette (SEC), based on the SapTrap CRISPR/Cas Toolkit.
    Depositor
    Bob Goldstein
    Application
    Genome Editing
    Vector Type
    Worm Expression
    Editing Type
    CRISPR
    Available Since
    June 23, 2017
    Availability
    Academic Institutions and Nonprofits only

  5. C. elegans miniMos transgenesis kit

    Plasmid Kit
    #1000000031
    Purpose
    Transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon.
    Depositor
    Erik Jorgensen
    Application
    Genome Editing
    Vector Type
    Worm Expression
    Editing Type
    Transposon
    Available Since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. Shen Lab DdCBE assembly kit

    Plasmid Kit
    #1000000212
    Purpose
    Generate DddA-derived cytosine base editors (DdCBEs).
    Depositor
    Bin Shen
    Application
    Genome Editing
    Vector Type
    Zebrafish Expression
    Editing Type
    Base Editing
    Available Since
    March 14, 2023
    Availability
    Academic Institutions and Nonprofits only

  7. MoBiFC

    Plasmid Kit
    #1000000222
    Purpose
    Modular bimolecular fluorescence complementation (MoBiFC) toolkit for analyzing protein-protein interactions in plants.
    Depositor
    Benjamin Field
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Plant Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only

  8. Zinc Finger Consortium Expression Vector and Modular Assembly Kit

    Plasmid Kit
    #1000000005
    Purpose
    Plasmids and strain to assemble Zinc finger nucleases (ZFNs) for performing genome engineering in a variety of organisms.
    Depositor
    Keith Joung
    Application
    Genome Editing
    Vector Type
    Bacterial Expression, Insect Expression, Mammalian Expression, Plant Expression
    Editing Type
    ZFN
    Available Since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  9. High Complexity Golden Gate Assembly Standards Kit (24 fragment lac cassette)

    Plasmid Kit
    #1000000221
    Purpose
    End-joining ligation fidelity and bias data predicts high accuracy junction sets for Golden Gate assembly, providing a positive control for complex assembly via blue/white screening in E. coli.
    Depositor
    Eric Cantor
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate
    Available Since
    June 1, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. Joung Lab REAL Assembly TALEN Kit

    Plasmid Kit
    #1000000017
    Purpose
    Restriction Enzyme And Ligation (REAL) TALEN assembly of TALE repeat arrays. Includes vector backbones for mammalian expression or in vitro transcription to produce TALE nuclease mRNAs.
    Depositor
    Keith Joung
    Application
    Genome Editing
    Vector Type
    In Vitro Transcription, Mammalian Expression
    Editing Type
    TALEN
    Available Since
    Nov. 4, 2011
    Availability
    Academic Institutions and Nonprofits only

  11. FX cloning Kit

    Plasmid Kit
    #1000000039
    Purpose
    Use fragment exchange (FX) cloning to generate constructs for screening protein expression in several organisms with a variety of N- and C-terminal tags.
    Depositor
    Eric Geertsma
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Bacterial Expression, Insect Expression, Mammalian Expression, Xenopus Expression, Yeast Expression
    Cloning Type
    FX Cloning
    Available Since
    April 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. Yamamoto Lab TALEN Accessory Pack

    Plasmid Kit
    #1000000030
    Purpose
    Supplemental kit for #1000000024 includes modified pFUS vectors to simplify Golden Gate assembly and mammalian expression/mRNA synthesis vectors containing unique shorter N- and C-terminal domains.
    Depositor
    Takashi Yamamoto
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    In Vitro Transcription, Mammalian Expression
    Editing Type
    TALEN
    Cloning Type
    Golden Gate
    Available Since
    March 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. The Expanded EasyClone-MarkerFree toolkit

    Plasmid Kit
    #1000000190
    Purpose
    Expansion of the EasyClone-MarkerFree Vector Set (#1000000098) with eight additional chromosomal loci for the stable integration of heterologous DNA into Saccharomyces cerevisiae by CRISPR/Cas9.
    Depositor
    Irina Borodina
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    USER
    Available Since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only

  14. Zinc Finger Consortium Expression Vector Kit v1.0

    Plasmid Kit
    #1000000010
    Purpose
    Plasmids and strain for the bacterial two-hybrid system used to screen Zinc finger nucleases (ZFNs).
    Depositor
    Keith Joung
    Application
    Genome Editing
    Vector Type
    Bacterial Expression, Insect Expression, Mammalian Expression, Plant Expression
    Editing Type
    ZFN
    Available Since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  15. Yeast GoldenBraid Cloning System and Toolkit

    Plasmid Kit
    #1000000138
    Purpose
    This toolkit allows generation of transcriptional units using GoldenBraid for integration in the S. cerevisiae genome at loci, YPRCΔ15 and YORWΔ22, to support high and stable transgene expression.
    Depositor
    Elena Caro Bernat
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (GoldenBraid)
    Available Since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  16. POMBOX MoClo YTK

    Plasmid Kit
    #1000000251
    Purpose
    Seamless and efficient assembly of single gene and multigene plasmids for genome integration in Schizosaccharomyces pombe.
    Depositor
    Tomáš Pluskal
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only

  17. LIC TAL Effector Assembly Kit

    Plasmid Kit
    #1000000023
    Purpose
    A TALE repeat unit library of 2-mer fragments that can be assembled via Ligation-independent cloning (LIC) into mammalian expression-ready TALEN constructs in 2 hierarchical assembly steps.
    Depositor
    Veit Hornung
    Application
    Genome Editing
    Vector Type
    Mammalian Expression
    Editing Type
    TALEN
    Available Since
    Jan. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. Hamdoun lab FP fusion plasmids

    Plasmid Kit
    #1000000033
    Purpose
    Generate N- and C-terminal fusions to mCherry, Cerulean, mCitrine, or EGFP for screening fluorescent protein fusion expression in organisms or cells including Zebrafish, Xenopus, and C. elegans.
    Depositor
    Amro Hamdoun
    Application
    Gene Expression and Labeling
    Vector Type
    Worm Expression, Xenopus Expression, Zebrafish Expression
    Available Since
    July 24, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. Myristoylated Kinase Library

    Plasmid Kit
    #1000000012
    Purpose
    Plasmids for human kinases and kinase-related ORFs, with myristoylation sequence and flag-epitope tag to each.
    Depositor
    William Hahn
    Application
    Gene Expression and Labeling
    Vector Type
    Mammalian Expression, Retroviral
    Available Since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  20. Yeast Prototrophy Kit

    Plasmid Kit
    #1000000085
    Purpose
    Plasmids designed to restore prototrophy and test metabolic capacity in common S. cerevisiae laboratory strains.
    Depositor
    Markus Ralser
    Application
    Gene Expression and Labeling
    Vector Type
    Yeast Expression
    Available Since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 101 - 120 of 166 results