163 results

1. Yeast GPCR-sensor Toolkit

   Plasmid Kit

   #1000000157

   Purpose

   The Yeast GPCR-sensor Toolkit includes 42 plasmids for constructing highly-tunable GPCR-based biosensors in yeast.

   Depositor

   Tom Ellis

   Available Since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

2. Zinc Finger Consortium Expression Vector and Modular Assembly Kit

   Plasmid Kit

   #1000000005

   Purpose

   Can be used to efficiently assemble zinc finger arrays via modular assembly (Sangamo, Barbas, and Toolgen). Also includes the bacterial two-hybrid (B2H) system used to screen arrays for activity.

   Depositor

   Keith Joung

   Available Since

   July 16, 2011

   Availability

   Academic Institutions and Nonprofits only

3. FusX TALEN assembly system

   Plasmid Kit

   #1000000063

   Purpose

   Golden Gate assembly of 15-16 RVD TALEN inserts in a single cloning step; designed to be used with high throughput robotics

   Depositor

   Stephen Ekker

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

4. Hamdoun lab FP fusion plasmids

   Plasmid Kit

   #1000000033

   Purpose

   Screen fluorescent protein fusion expression in organisms or cells in which an exogenous mRNA can be injected and expressed, e.g. zebrafish, sea urchin, frog (Xenopus), or worm (C. elegans)

   Depositor

   Amro Hamdoun

   Available Since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

5. Yeast GoldenBraid Cloning System and Toolkit

   Plasmid Kit

   #1000000138

   Purpose

   Modular cloning system for generation of high expression transcriptional units; Integrates in yeast (S. cerevisiae) genome at two different loci supporting high and stable transgene expression

   Depositor

   Elena Caro Bernat

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

6. Zinc Finger Consortium Expression Vector Kit v1.0

   Plasmid Kit

   #1000000010

   Purpose

   Contains plasmids and strains for the bacterial two-hybrid (B2H) system used to screen zinc finger arrays for activity. Also called the Modular Assembly Accessory Reagents Kit.

   Depositor

   Keith Joung

   Available Since

   July 16, 2011

   Availability

   Academic Institutions and Nonprofits only

7. Yamamoto Lab TALEN Accessory Pack

   Plasmid Kit

   #1000000030

   Purpose

   Contains modified pFUS array vectors and destination vectors that are designed for use with the Golden Gate TALEN and TAL Effector Kit.

   Depositor

   Takashi Yamamoto

   Available Since

   March 28, 2013

   Availability

   Academic Institutions and Nonprofits only

8. POMBOX MoClo YTK

   Plasmid Kit

   #1000000251

   Purpose

   Use POMBOX for seamless and efficient assembly of single gene and multigene plasmids for genome integration in S. pombe.

   Depositor

   Tomáš Pluskal

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

9. SapTrap Kit

   Plasmid Kit

   #1000000077

   Purpose

   SapTrap: modular toolkit for building CRISPR/Cas9 targeting vectors to insert genetic tags in C. elegans genome. 21 vector assembly plasmids, Unc-32::GFP targeting vector, 5 Cre/Flp expression vectors

   Depositor

   Erik Jorgensen

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

10. MetClo DNA Assembly Kit

    Plasmid Kit

    #1000000153

    Purpose

    MetClo DNA Assembly Kit allows modular, hierarchical assembly of DNA fragments using IIS restriction enzyme. Includes 3 E.coli strains for BsaI, BpiI and LguI-based MetClo assembly.

    Depositor

    Christopher A. O'Callaghan

    Available Since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

11. FX cloning Kit

    Plasmid Kit

    #1000000039

    Purpose

    Insert sequence of interest into a cloning vector and subclone into expression vectors, containing a variety of N- and C-terminal tags; for use in both prokaryotic and eukaryotic systems

    Depositor

    Eric Geertsma

    Available Since

    April 2, 2014

    Availability

    Academic Institutions and Nonprofits only

12. Target Accelerator Pan-Cancer Mutant Collection

    Plasmid Kit

    #1000000103

    Purpose

    Panel of cancer alleles that have undergone basic, functional screening; full-length, human clones (454 mutant and 177 corresponding wild-type) in the Gateway cloning system

    Depositor

    Jesse Boehm

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

13. LIC TAL Effector Assembly Kit

    Plasmid Kit

    #1000000023

    Purpose

    LIC (ligation-independent cloning) TALE repeat unit library of 2-mer fragments for assembly in an expression ready TALEN construct in 2 hierarchical assembly steps. Semi‐automated TALE generation

    Depositor

    Veit Hornung

    Available Since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

14. Joung Lab REAL Assembly TALEN Kit

    Plasmid Kit

    #1000000017

    Purpose

    Used to assemble any combination of TALE repeats into arrays of any desired length using the REAL, REAL-Fast or FLASH assembly methods.

    Depositor

    Keith Joung

    Available Since

    Nov. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

15. Yeast Prototrophy Kit

    Plasmid Kit

    #1000000085

    Purpose

    Plasmids derived from the pHLUM mini-chromosome, designed to restore prototrophy (eg HIS3, LEU2, URA3, MET17 or LYS2 genes) in common yeast (S. cerevisiae) laboratory strains.

    Depositor

    Markus Ralser

    Available Since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

16. Mobius Assembly Vector Toolkit

    Plasmid Kit

    #1000000134

    Purpose

    Mobius Assembly: Uses a two-level approach for transcriptional unit (TU) and multi-TU augmentation. Each level is comprised of four Acceptor Vectors. Compatible with Golden Gate parts

    Depositor

    Naomi Nakayama

    Available Since

    March 13, 2018

    Availability

    Academic Institutions and Nonprofits only

17. CRISPR-Cas9 Gateway System for Physcomitrella patens

    Plasmid Kit

    #1000000151

    Purpose

    CRISPR-Cas9 Gateway System is a collection of 24 plasmids providing a modular, efficient CRISPR/Cas9 editing system for accurate and customizable repair in Physcomitrella patens.

    Depositor

    Magdalena Bezanilla

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

18. RAS Pathway 2.0 Clone Collection

    Plasmid Kit

    #1000000070

    Purpose

    Collection contains 180 genes in the “RAS pathway version 2.0”, a crowdsourced pathway map of the RAS signaling pathway; For use with Gateway cloning or FNLCR Combinatorial Cloning Platform

    Depositor

    Dominic Esposito

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pDGE Dicot Genome Editing Kit

    Plasmid Kit

    #1000000084

    Purpose

    Golden Gate cloning-based assembly of constructs for CRISPR genome editing in dicotyledonous plants. Consists of "genome editing recipient" and "sgRNA shuttle" vectors.

    Depositor

    Johannes Stuttmann

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

20. Myristoylated Kinase Library

    Plasmid Kit

    #1000000012

    Purpose

    190 human kinases and kinase-related open reading frames (ORFs) cloned into a retroviral vector which adds a myristoylation sequence and flag-epitope tag to each ORF.

    Depositor

    William Hahn

    Available Since

    July 16, 2011

    Availability

    Academic Institutions and Nonprofits only