161 results
-
Plasmid Kit#1000000190PurposeThe Expanded EasyClone-MarkerFree toolkit is a new set of 8 validated chromosomal loci for the stable integration of heterologous DNA into baker’s yeast Saccharomyces cerevisiae.DepositorAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only
-
Hamdoun lab FP fusion plasmids
Plasmid Kit#1000000033PurposeScreen fluorescent protein fusion expression in organisms or cells in which an exogenous mRNA can be injected and expressed, e.g. zebrafish, sea urchin, frog (Xenopus), or worm (C. elegans)DepositorAvailable SinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
B. subtilis Single Gene Deletion Library--Kanamycin
Plasmid Kit#1000000115PurposeB. subtilis Single Gene Deletion Library - Kanamycin: Arrayed deletion library in which each non-essential gene has been individually replaced with a kanamycin resistance cassette.DepositorAvailable SinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
EXPRESSYALI
Plasmid Kit#1000000245PurposeThe EXPRESSYALI Set is a complete kit for the introduction of genes into Yarrowia chromosomal loci. The kit includes six part plasmids, 12 marker-free integrative vectors, and 11 gRNA plasmids.DepositorAvailable SinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Endy/Shearwin pOSIP plasmid set
Plasmid Kit#1000000035Purpose11 Plasmids for use in clonetegration: method for decreasing time and effort needed for integration of DNA into prokaryotic chromosomes. Facilitates cloning, expression of difficult genetic elementsDepositorAvailable SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
SapTrap-SEC kit for C. elegans genome engineering
Plasmid Kit#1000000109PurposeBuild sgRNA and homologous repair templates that incorporate self-excising drug selection cassette (SEC), using SapTrap cloning method. Addition of AAG junction.DepositorAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
Yeast GPCR-sensor Toolkit
Plasmid Kit#1000000157PurposeThe Yeast GPCR-sensor Toolkit includes 42 plasmids for constructing highly-tunable GPCR-based biosensors in yeast.DepositorAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
MetClo DNA Assembly Kit
Plasmid Kit#1000000153PurposeMetClo DNA Assembly Kit allows modular, hierarchical assembly of DNA fragments using IIS restriction enzyme. Includes 3 E.coli strains for BsaI, BpiI and LguI-based MetClo assembly.DepositorAvailable SinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Zinc Finger Consortium Expression Vector and Modular Assembly Kit
Plasmid Kit#1000000005PurposeCan be used to efficiently assemble zinc finger arrays via modular assembly (Sangamo, Barbas, and Toolgen). Also includes the bacterial two-hybrid (B2H) system used to screen arrays for activity.DepositorAvailable SinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
The Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit
Plasmid Kit#1000000210PurposeToolkit contains Cas9-gRNA vectors, integration plasmids, and organellar localization markers as a set of plasmids for Cas9-mediated integration of tagged genes and markers into the yeast genome.DepositorAvailable SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
B. subtilis CRISPRi Essential Gene Knockdown Library
Plasmid Kit#1000000116PurposeB. subtilis CRISPRi Essential Gene Knockdown Library: Arrayed library that uses CRISPRi to sterically hinder transcription and therefore knock down expression of essential genes.DepositorAvailable SinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
Yeast GoldenBraid Cloning System and Toolkit
Plasmid Kit#1000000138PurposeModular cloning system for generation of high expression transcriptional units; Integrates in yeast (S. cerevisiae) genome at two different loci supporting high and stable transgene expressionDepositorAvailable SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Zinc Finger Consortium Expression Vector Kit v1.0
Plasmid Kit#1000000010PurposeContains plasmids and strains for the bacterial two-hybrid (B2H) system used to screen zinc finger arrays for activity. Also called the Modular Assembly Accessory Reagents Kit.DepositorAvailable SinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
ScEnSor Kit
Plasmid Kit#1000000215PurposeThis three-step (Build - Transform - Assess) Saccharomyces cerevisiae kit is comprised of two modules (Engineering and Biosensor) that can be used independently or in combination.DepositorAvailable SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
CRISPRainbow Kit for Multiplex Labeling
Plasmid Kit#1000000079PurposeCRISPR/Cas9 to tag up to 7 different genomic locations. Unique labeling system allows scientists to track DNA movement in live cells and study the structure of the genome in real time.DepositorAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mobius Assembly Vector Toolkit
Plasmid Kit#1000000134PurposeMobius Assembly: Uses a two-level approach for transcriptional unit (TU) and multi-TU augmentation. Each level is comprised of four Acceptor Vectors. Compatible with Golden Gate partsDepositorAvailable SinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Golden GATEway Cloning Kit
Plasmid Kit#1000000054PurposeCombinatorial assembly method that combines Golden Gate (Type IIs endonucleases) cloning with Multisite Gateway (recombination) cloning to construct complex plasmids in an efficient and modular wayDepositorAvailable SinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
POMBOX MoClo YTK
Plasmid Kit#1000000251PurposeUse POMBOX for seamless and efficient assembly of single gene and multigene plasmids for genome integration in S. pombe.DepositorAvailable SinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
Target Accelerator Pan-Cancer Mutant Collection
Plasmid Kit#1000000103PurposePanel of cancer alleles that have undergone basic, functional screening; full-length, human clones (454 mutant and 177 corresponding wild-type) in the Gateway cloning systemDepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Wnt Open Source
Plasmid Kit#1000000022PurposeOpen Source Wnt: 95 plasmid library of all 19 Wnt clones. Collection contains full-length open reading frames (ORFs) for every human Wnt ligand.DepositorAvailable SinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only