Pro-Code Vector Kits
(Kit #
1000000177, 1000000197
)
Depositing Lab: Brian Brown
Kit #1000000177: The Pro-Code Vector Kit is a set of 120 lentiviral vector plasmids, each with a unique protein barcode (Pro-Code) and gRNA cloning site.These plasmids can be used for CRISPR screens and for tracking cell clones or populations.
Kit #1000000197: The Nuclear Pro-Code Vector Kit is a set of 75 lentiviral vector plasmids, each with a unique barcode (Pro-Code) and gRNA cloning site. In this collection, each Pro-Code is linked to a nuclear-localizing mCherry protein, which results in the Pro-Codes being concentrated in the nucleus.
These kits will be sent as bacterial glycerol stocks in 96-well plate format.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Kit | 1000000177 | Pro-Code Vector Kit | 1 | $ 640 | Add to Cart | |
| Kit | 1000000197 | Nuclear Pro-Code Vector Kit | 1 | $ 400 | Add to Cart | |
Original Publications
Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD. Cell. 2018 Nov 1; doi: 10.1016/j.cell.2018.09.022. PubMed PMID: 30343902.
Spatial CRISPR genomics identifies regulators of the tumor microenvironment. Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S, Pe'er D, Merad M, Brown BD. Cell. 2022 Mar 31;185(7):1223-1239.e20. doi: 10.1016/j.cell.2022.02.015. PubMed PMID: 35290801.
Description
Kit 1000000177: Pro-Code Vector Kit This collection contains a set of 120 lentiviral vector plasmids. Each plasmid encodes a unique protein barcode (Pro-Code), which can be identified on cells by staining with a cocktail of just 10 antibodies. Each Pro-Code is comprised of a mutant version of the human NGFR gene (dNGFR) fused to a sequence encoding three different linear epitopes. The Pro-Code is expressed from the human EF1alpha promoter. The lentiviral vector also contains the U6 promoter and a unique cloning site for insertion of a CRISPR guide RNA. The Pro-Code vectors can be used to carry out pooled CRISPR screens in which 120 different CRISPR-carrying cell populations can be resolved by staining cells with a 10 antibody cocktail and performing single cell flow cytometry, mass cytometry, or by multiplex imaging. The Pro-Code vectors can also be used for tracking cell clones or cell populations.
Kit 1000000197: Nuclear Pro-Code Vector Kit This collection is a set of 75 lentiviral vector plasmids, each with a gRNA cloning site and a unique barcode (Pro-Code). The Pro-Codes in these plasmids are linked to a nuclear-localizing mCherry protein, resulting in the Pro-Codes concentrating in the nucleus. These plasmids can be used for CRISPR screens and for tracking cell clones or populations. The nuclear Pro-Codes are well suited for in situ imaging detection.
- Pro-Code and Nuclear Pro-Code Vector Kit overview and experimental approaches.
Kit Documentation
Pro-Code_Kit_Methodology.pdf 548.5Â KBHow to Cite these Kits
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
Kit 1000000177: Pro-Code Vector Kit
For your Materials and Methods section:
“The Pro-Code Vector Kit was a gift from Brian Brown (Addgene kit #1000000177).”
For your Reference section:
Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD. Cell. 2018 Nov 1; doi: 10.1016/j.cell.2018.09.022. PubMed PMID: 30343902.
Kit 1000000197: Nuclear Pro-Code Vector Kit
For your Materials and Methods section:
“The Nuclear Pro-Code Vector Kit was a gift from Brian Brown (Addgene kit #1000000197).”
For your Reference section:
Spatial CRISPR genomics identifies regulators of the tumor microenvironment. Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S, Pe'er D, Merad M, Brown BD. Cell. 2022 Mar 31;185(7):1223-1239.e20. doi: 10.1016/j.cell.2022.02.015. PubMed PMID: 35290801.
Pro-Code Vector Kit - #1000000177
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right, as well as lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Ampicillin |
Inventory
Kit Plate Map - #1000000177
Nuclear Pro-Code Vector Kit - #1000000197
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Ampicillin |