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pQTEV-RPL9

Plasmid

#31334

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

ribosomal protein L9 (RPL9 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pETDuet-1∆HisTag_Nef_MCS2

Plasmid

#66079

Purpose

Single vector system expressing NEF allowing myristoylation of recombinant protein produce in Ecoli system

Depositor

Dieter Willbold

Article

Gluck et al PLoS One. 2010 Apr 9;5(4):e10081. doi: 1

Insert

Nef

Tags

6xHis and HRV 3C Site

Expression

Bacterial

Promoter

T7-lacO

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only
pOPINVH

Plasmid

#26041

Depositor

Ray Owens

Article

Nettleship et al Protein Expr Purif. 2008 Nov . 62(1):83-

Insert

u-LacZ-KHHHHHH

Tags

KHHHHHH and u-phosphatase secretion leader

Expression

Bacterial, Insect, and Mamm…

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pJS183

Plasmid

#134142

Purpose

Bacterial expression of GFP-Ska1 and Ska2

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-GFP-human Ska1; human Ska2

Tags

GFP

Expression

Bacterial

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Neo-PuroDTK

Plasmid

#84036

Purpose

PiggyBac cassette contains Neomycin, puromycin and TK genes

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Expression

Bacterial and Mammalian

Promoter

CAG promoter

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-GGG-ELP(120nm)-Cys

Plasmid

#91572

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-ELP(120nm)-Cys

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Promoter

T7

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-DEST-FLAG

Plasmid

#119754

Purpose

Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal FLAG tag.

Depositor

Andrew Jackson, Martin Reijns

Type

Empty backbone

Tags

FLAG and GST

Expression

Bacterial

Promoter

tac promoter

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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CD Ugi4

Plasmid

#177196

Purpose

Greengate entry vector pGGC000 modified to contain bladderwort insulator 4

Depositor

Wayne Parrott

Article

Park et al bioRxiv 2021.11.04.467280

Insert

Utricularia gibba insulator 4

Expression

Bacterial and Plant

Available Since

June 28, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pAKgfplux2

Plasmid

#14084

Depositor

Attila Karsi

Article

Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

Insert

Bacterial luciferase-lacIq repressor gene

Expression

Bacterial

Available Since

March 12, 2007

Availability

Academic Institutions and Nonprofits only
pFA6a-hphMX6-3nmt1

Plasmid

#105162

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

Insert

hphMX6-3nmt1

Tags

3nmt1

Expression

Bacterial and Yeast

Promoter

3nmt1

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pYTK080

Plasmid

#65187

Purpose

Encodes ZeocinR as a Type 6 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

ZeocinR

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pBS hTSP-4

Plasmid

#13001

Depositor

Jack Lawler

Article

Lawler et al J Biol Chem. 1995 Feb 10. 270(6):2809-14

Insert

TSP-4 (THBS4 Human)

Expression

Bacterial

Available Since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only
pET29a-hNABP2

Plasmid

#128307

Purpose

Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)

Tags

His

Expression

Bacterial

Mutation

Y85C- please see depositor comments below

Promoter

T7

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pPROEX Hsp104 (1-575)

Plasmid

#1318

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (1-575) (HSP104 Budding Yeast)

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

aa1-575 of Hsp104

Available Since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only

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pBAD33.1-DpnII

Plasmid

#127413

Purpose

Expresses DpnII restriction endonuclease in bacteria

Depositor

Douglas Feldman

Insert

DpnII

Expression

Bacterial

Promoter

pBAD

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLC133

Plasmid

#198639

Purpose

Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

Depositor

David Bikard

Article

Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

Insert

dCas9

Tags

3x-FLAG

Expression

Bacterial

Promoter

pTet

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET-Duet1-His-TEV-mCherry-OPTN WT

Plasmid

#190191

Purpose

Plasmid for the expression of His-TEV-mCherry-OPTN WT. Internal reference: SMC797

Depositor

Sascha Martens

Article

Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

optineurin (OPTN Human)

Tags

6xHis, TEV, and mCherry

Expression

Bacterial

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pETDuet-1_VNp-mNeongreen_CydAB-mCherry

Plasmid

#182391

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen and cydAB-mCherry fusions

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Inserts

VNp-mNeongreen-His
cydAB-mCherry

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pH6HTC_RBFOX2-Halo

Plasmid

#175304

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

RBFOX2 (RBFOX2 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pUMV19

Plasmid

#67161

Purpose

inducible operation of the mevalonate pathway in E. coli

Depositor

Tomohisa Kuzuyama

Article

Takagi et al J Bacteriol. 2000 Aug;182(15):4153-7.

Insert

the mevalonate pathway genes

Expression

Bacterial

Promoter

lacZ

Available Since

Oct. 27, 2015

Availability

Academic Institutions and Nonprofits only

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