33,526 results

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pRSFDuet1_CylLL_CylM

Plasmid

#208759

Purpose

Expresses modified cytolysin L (mCylLL) in E. coli

Depositor

Wilfred van der Donk

Publication

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CylLL
CylLM

Tags

Hisx6 Tag

Expression

Bacterial

Promoter

T7

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSFDuet1_CoiA1_CoiB

Plasmid

#208761

Purpose

Precursor peptide CoiA1 and synthase CoiB, for the co-expression of CoiA1 in E.coli.

Depositor

Wilfred van der Donk

Publication

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CoiA1
CoiB

Tags

Hisx6 Tag and SUMO

Expression

Bacterial

Mutation

Changed Ser 20 to Asn, Changed Arg 84 to Lys, bot…

Promoter

T7

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD04

Plasmid

#208258

Purpose

Production of ent-kaurene in E. coli MEV20

Depositor

Christopher Voigt

Publication

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
blr2150

Expression

Bacterial

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pET24a MDH_AQUAR

Plasmid

#204239

Purpose

Bacterial expression of A. arcticum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

Depositor

Malate dehydrogenase CUREs Community (MCC)

Insert

MDH_AQUAR

Tags

6X His

Expression

Bacterial

Promoter

Lac

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a MDHC_YEAST

Plasmid

#204232

Purpose

Bacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

Depositor

Malate dehydrogenase CUREs Community (MCC)

Insert

MDHC_YEAST (MDH2 Budding Yeast)

Tags

6X His

Expression

Bacterial

Promoter

Lac

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 R130A wgMDH

Plasmid

#204266

Purpose

Bacterial expression of R130A wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Publication

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R130A

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pMB1-A-TelR

Plasmid

#204979

Purpose

Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

Depositor

Veronica Godoy-Carter

Publication

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Added replications elements for Acinetobacter spe…

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT194

Plasmid

#202293

Purpose

Type IIS cloning plasmid for LPattB5 integrations w/ OriT

Depositor

Christopher Voigt

Publication

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

sfGFP

Expression

Bacterial

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pET19-MTdTwt

Plasmid

#207460

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

Depositor

Jay Keasling

Publication

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

MTdTwt (Dntt Mouse)

Tags

10xHis-MBP

Expression

Bacterial

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMYCO1_SpdCas9_pmcDA1

Plasmid

#204615

Purpose

CRISPR cytosine deaminase replicative plasmid for Mycoplasma mycoides subsp. mycoides

Depositor

Pascal Sirand-Pugnet

Publication

Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET28C-SARS-CoV-2-Nucleocapsid

Plasmid

#204402

Purpose

E. coli expression of full-length SARS-CoV-2 nucleocapsid

Depositor

Samie Jaffrey

Publication

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

SARS-CoV-2 nucleocapsid (N SARS-CoV-2)

Tags

6x-His

Expression

Bacterial

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-DDX4LCD

Plasmid

#204408

Purpose

E. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Publication

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

DDX4(1-236) (DDX4 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDA303

Plasmid

#195199

Purpose

Overexpressing 6His-TetR in E. coli.

Depositor

Rahul Sarpeshkar

Publication

Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

Insert

TetR

Tags

6xHis

Expression

Bacterial

Promoter

T5 promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCW-LIC-N8C_Fab3a

Plasmid

#203689

Purpose

Expresses N8C_Fab3a

Depositor

Brenda Schulman

Publication

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab3a

Expression

Bacterial

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pMB1-A

Plasmid

#190114

Purpose

Level 1 MoClo plasmid for A. baumannii

Depositor

Veronica Godoy-Carter

Publication

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pIA028

Plasmid

#200419

Purpose

thrC::(PAmCherry1 erm2),for PAmCherry1 fusions.PALM integration vector for B. subtilis. Includes PAmCherry1 photoactivatable FP with multi cloning sites (MCS) for fast and interchangeable cloning

Depositor

Rut Carballido-López

Publication

Altinoglu et al Microbiol Spectr. 2024 Nov 4;12(12):e016

Type

Empty backbone

Tags

PAmCherry1

Expression

Bacterial

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET-jAspSnFR3

Plasmid

#203458

Purpose

jAspSnFR3 in T7 bacterial expression vector

Depositor

Lucas Sullivan

Publication

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET-WHaloCaMP1b-EGFP

Plasmid

#205305

Purpose

Expression of WHaloCaMP1b-EGFP fusion in E. coli

Depositor

Eric Schreiter

Publication

Farrants et al Nat Methods. 2024 Oct;21(10):1916-1925.

Insert

WHaloCaMP1b-EGFP

Expression

Bacterial

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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dCas9-CRY2Clust-BRD4ΔN

Plasmid

#200968

Purpose

induction of phase-separated condensate mediated by BRD4dN-IDR

Depositor

Jian Xu

Publication

Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

Insert

BRD4dN

Expression

Bacterial and Mammalian

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-UFBP1

Plasmid

#204558

Purpose

Expresses GST-tagged UFBP1 in E.coli

Depositor

Masaaki Komatsu

Publication

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Expression

Bacterial

Mutation

none

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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