34,239 results
-
Plasmid#215457PurposeEncodes the bacterial collagen-binding protein CNA35 with N-terminal HaloTag.DepositorInsertCNA35
TagsHaloTagExpressionBacterialMutationIncludes stop codonAvailable sinceMay 29, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMIIS638
Plasmid#238210PurposeExpresses native SpTasH TIGR locus. CmRDepositorInsertSpTasH
ExpressionBacterialAvailable sinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTW386c
Plasmid#237321Purposeexpresses O. sativa Cpn60α Cpn60β Cpn20-2 Raf1 Raf2 RbcX Bsd2DepositorInsertO. sativa Cpn60α Cpn60β Cpn20-2 Raf1 Raf2 RbcX Bsd2
ExpressionBacterialAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTW334i
Plasmid#237319Purposeexpresses A. thaliana Cpn60α Cpn60β Cpn20 Raf2 RbcX Bsd2 N. tabacum Raf1DepositorInsertA. thaliana Cpn60α Cpn60β Cpn20 Raf2 RbcX Bsd2 N. tabacum Raf1
ExpressionBacterialAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY53
Plasmid#221709PurposeEmpty Cas12a vector for multiplex gene editing in fungiDepositorTypeEmpty backboneUseCRISPR; AspergillusExpressionBacterialAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAK004
Plasmid#233641PurposeFor bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagA.DepositorInserteSpCas9(1.1) with c-terminal VitelloTagA
Tags6-HisExpressionBacterialAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
p344_DG
Plasmid#234674PurposeGolden gate domesticated pET28b with His6-3CDepositorTypeEmpty backboneTagsHis6-3CExpressionBacterialMutationNoAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p356_DG
Plasmid#234699PurposeGolden gate domesticated pET28b for scarless cloning with His10-MBP-3CDepositorTypeEmpty backboneTagsHis10-MBP-3CExpressionBacterialMutationNoAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p353_DG
Plasmid#234696PurposeGolden gate domesticated pET28b for scarless cloning with His10-3CDepositorTypeEmpty backboneTagsHis10-3CExpressionBacterialMutationNoAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET23a-His-TEV-HaloTag-linker-SpyCatcher3
Plasmid#235150PurposeBacterial expression of His-tagged HaloTag-linker-SpyCatcher3 separated by a TEV protease recognition site.DepositorInsertHaloTag linked to SpyCatcher3
TagsHisExpressionBacterialPromoterT7Available sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_N-myc
Plasmid#234045PurposeBacterial expression of N-terminally 6His-tagged N-myc; includes TEV cleavage siteDepositorAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_MAX
Plasmid#234044PurposeBacterial expression of N-terminally 6His-tagged MAX; includes TEV cleavage siteDepositorAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL34
Plasmid#227431PurposeKanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)DepositorInserttdTomato
UseCRISPRExpressionBacterialAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIA123
Plasmid#234038PurposeE. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.DepositorInsertsCas9
sgRNA
UseCRISPRExpressionBacterialPromoterPgdh and PxylAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mGold2t
Plasmid#231766PurposeExpression of mGold2t in bacterial cellsDepositorInsertmGold2t
ExpressionBacterialMutationmGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…PromoterSynthetic promoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
HIS-HA-CMYC P53
Plasmid#233738Purposeexpresses HIS-HA-CMYC triple tagged P53 for pulldown assayDepositorAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZP06
Plasmid#232320PurposepZP05 was constructed by cloning the oriFn-Pfdx-E-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, creating a conditional plasmid with replication controlled by Pfdx-E.DepositorInsertPfdx-E
ExpressionBacterialAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
ZIKV isolate PRVABC59 NS3 helicase domain
Plasmid#228664PurposeBacterial expression of the Zika virus NS3 helicaseDepositorInsertZika virus NS3 helicase
TagsHis-GST-TEVExpressionBacterialAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain
Plasmid#228663PurposeBacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)DepositorInsertZika virus portion of NS2B protease coexpressed with NS3 protease
TagsHis-GST-TEVExpressionBacterialAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pEND-400_Cacnes_CAMP2
Plasmid#225606PurposeSuicide C. acnes vector derivative of pEND-350 optimized for recombination into the CAMP2 locus, with BxbI recombination sites flanking EryR resistance cassetteDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only