33,532 results
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Plasmid#199616PurposeEncodes DARPin-FLAG 27G2 for bacterial expression and column purificationDepositorInsertDARPin-FLAG 27G2
Tags8xHis and FLAGExpressionBacterialPromoterT5Available sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDSP32
Plasmid#199374PurposeEncodes dual cistronic nuclear localized jGCaMP7s and mScarlet-I optimized for C. elegans expressionDepositorInsertNLS::jGCaMP7s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
ExpressionBacterialMutationno mutations, except added subcellular localizati…Available sinceApril 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGST2-GST-TEV-OPTN
Plasmid#198428PurposeUsed for the expression and purification of OPTN (WT) (SMC Internal No. 1587)DepositorAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDSP1
Plasmid#199384PurposeKanamycin resistant cloning vector backbone for pDSP23, pDSP31-33, pHJL1-4, pSiM1-4DepositorTypeEmpty backboneExpressionBacterialMutationCloning vector backboneAvailable sinceApril 20, 2023AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pMUT2-pLac-mCherry
Plasmid#192859PurposepMUT2 based vector for sensing lactic acid in E. coli Nissle 1917DepositorInsertlldR operon
ExpressionBacterialAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-pTcpP-mCherry
Plasmid#192861PurposepMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917DepositorInsertTcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids
ExpressionBacterialAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-VtrA-mCherry
Plasmid#192862PurposepMUT2 based vector for sensing human secondary bile acids in E. coli Nissle 1917DepositorInsertVtrA-CadC fusion protein and cognate promoter inducible by secondary bile acids
ExpressionBacterialAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Cx43-BioID2-BioID2-HA
Plasmid#176743PurposeExpresses Connexin-43 fused with BioID2 at the C-terminus in astrocytesDepositorInsertCx43-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDule- Mj Acd A9
Plasmid#197651PurposeE. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pairDepositorInsertsMj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA
TagsNoneExpressionBacterialMutationY32A, L65D, F108L, Q109E, D158S, L162TPromoterGlnS and lppAvailable sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
His SUMO TEV SPOP 1-374
Plasmid#197015PurposeE. coli expression vectorDepositorAvailable sinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
FCM101
Plasmid#194298PurposeE. coli expression vector for monobody adaptor, FCM101, that bind to the Fc region of mouse IgG1 subclass. It contains a single Cys residue at the C-terminus for chemical conjugation, N-terminal His6DepositorInsertFCM101
TagsHis tag, FLAG tagExpressionBacterialPromoterT7Available sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-ALFA
Plasmid#198301PurposeProduces PVCs retargeted with ALFA tagDepositorInsertPVCpnf_pvc13-ALFA
ExpressionBacterialAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-MoonTag
Plasmid#198299PurposeProduces PVCs retargeted with MoonTagDepositorInsertPVCpnf_pvc13-MoonTag
ExpressionBacterialAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro
Plasmid#198441PurposeActivity/inhibition assays, 19F-NMRDepositorInsertglycine linked Zika virus NS2B-NS3
TagsHis6-TEVExpressionBacterialMutationR95*A,PromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE80l A-CC-A
Plasmid#196478PurposeProtein expression of SpyTag-ELP-CarHCC-ELP SpyTag, with GRGD site linked with (GGGGS)2 before CarHCC and after first ELPDepositorInsertA-CC-A
ExpressionBacterialPromoterT7Available sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHis-TEV-Nsp15
Plasmid#187656PurposeE. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tagDepositorInsertNsp15 (N Severe acute respiratory syndrome coronavirus 2)
TagsTEV protease cleavable His6-tagExpressionBacterialMutationnonePromoterT7lacAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDx_mRuby3-SYFP2
Plasmid#189758PurposeDual expression vector for bacteria and mammalian cells producing mRuby3-SYFP2 fusion protein for FRET analysisDepositorInsertmRuby3-SYFP2
Tags6xHisExpressionBacterial and MammalianPromoterCMV & RhaAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGMC00018 (aka pRC0082)
Plasmid#195320PurposeMinimal plasmid backbone with multiple cloning site for HDR donor DNA constructionDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterial and MammalianAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
IRE-SunTag
Plasmid#196920PurposeThe 5UTR of the ferritin heavy chain mRNA was cloned upstream of the SunTag-Renilla coding sequence in the ΔIRE-SunTag reporterDepositorInsertRenilla luciferase
ExpressionBacterial and MammalianAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB222
Plasmid#170012PurposeEncodes LacI-controlled expression of surface-displayed LL-37 and PvirK-mNeonGreen reporterDepositorPublicationInsertsmNeonGreen
LL-37
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only