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3185 pBS Bax cDNA

Plasmid

#8754

Depositor

Stanley Korsmeyer

Article

Knudson et al Science 1995 Oct 6;270(5233):96-9.

Insert

Bax (Bax Mouse)

Expression

Bacterial

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pGEX-EGFAB(H306Y)

Plasmid

#25771

Depositor

Johann Deisenhofer

Article

McNutt et al J Biol Chem. 2009 Apr 17. 284(16):10561-

Insert

LDL receptor EGFAB (LDLR Human)

Tags

GST

Expression

Bacterial

Mutation

His 306 to Tyr

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
M62 pPET28a-TEV-human beta cat domain N(1-137)

Plasmid

#17203

Depositor

Randall Moon

Insert

beta catenin (CTNNB1 Human)

Tags

HIS

Expression

Bacterial

Mutation

amino-terminal residues 1-137

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
SPIN2B (SPIN2BA-c011)

Plasmid

#98238

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

SPIN2BA (SPIN2B Human)

Tags

TEV-His6-Flag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pFP.R371

Plasmid

#138241

Purpose

Expresses the split mCherry reporter interrupted by the MP-M-DnaB artificially mini-intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(NGK).MP-M-DnaB*.(TEL)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pWT046a

Plasmid

#107897

Purpose

W2.2, W2.4-1 plasmid. Contains PTetO-SD8-BE2, PBAD-sgRNA3 and is part of CAMERA 2.2 and 2.4

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-BE2, PBAD-sgRNA3

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX RASGAP(N)-SH2

Plasmid

#46432

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Gap/RASGAP(N) (RASA1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 168-283

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pAN-PA2-RFP

Plasmid

#62248

Purpose

reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

mRFP

Expression

Bacterial

Promoter

pA2

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX CblB-SH2

Plasmid

#46413

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

CblB (CBLB Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 42-359

Promoter

Tac

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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Gi-c009

Plasmid

#173079

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

GNAI1 (GNAI1 Human)

Tags

mCitrine

Expression

Bacterial

Mutation

full length, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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CoV-2-NSP3-c002

Plasmid

#173084

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

rep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))

Expression

Bacterial

Mutation

E1084-E1192, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSEVAT7Z1A

Plasmid

#58300

Purpose

GeneGuard vector cassette: thyA, ColE2 COR, Zeta toxin; with ampR as cargo.

Depositor

Tom Ellis

Article

Wright et al ACS Synth Biol. 2014 May 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-NEMOD311N

Plasmid

#11968

Depositor

Jon Ashwell

Article

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMOD311N (IKBKG Human)

Tags

GST

Expression

Bacterial

Mutation

Asp311 to Asn

Available Since

July 13, 2006

Availability

Academic Institutions and Nonprofits only
OR gate

Plasmid

#44451

Depositor

Drew Endy

Article

Bonnet et al Science. 2013 May 3;340(6132):599-603. d

Insert

OR-gate

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only

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pGex-3x MKK4

Plasmid

#14617

Depositor

Roger Davis

Article

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

MKK4 (MAP2K4 Human)

Tags

GST

Expression

Bacterial

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pMRE166

Plasmid

#118514

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Expression

Bacterial

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET his-mRFP

Plasmid

#113553

Purpose

For bacterial expression and purification of mRFP

Depositor

Jeanne Stachowiak

Article

DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

Insert

his-mRFP

Expression

Bacterial

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046879

Plasmid

#149238

Purpose

Bacterial expression plasmid for SARS-CoV-2 nsp2

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available Since

May 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-MK1

Plasmid

#29638

Depositor

Bruce Tempel

Insert

Mk1 (Kcna1 Mouse)

Expression

Bacterial

Available Since

May 5, 2011

Availability

Academic Institutions and Nonprofits only
pCB410 (His-Ub-Ate1-4 1A7B)

Plasmid

#104339

Purpose

Express His Tag Ate1-4 1A7B

Depositor

Alexander Varshavsky

Insert

ATE1 (Ate1 Mouse)

Tags

6xHis-Ub

Expression

Bacterial

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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