8,223 results
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Plasmid#160025PurposeIntegrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Uracil selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only
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pXP420 v2 (repaired Amp promoter)
Plasmid#86920PurposeShuttle vector to facilitate gene expression for metabolic engineering in S. cerevisiae. Contains TEF1 promoter and CYC1 terminator for insertion of gene of interest. Contains HIS3 selection marker.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastPromoterTEF1Available SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEK20
Plasmid#210582PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdGAPDHpro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastPromoterBdGAPDHproAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS305-mAID-LaM4
Plasmid#198414PurposeExpress mAID-Nb(LaM4) under the control of ADH1 promoter in budding yeastDepositorInsertmAID-Nb (LaM4)
ExpressionYeastPromoterADH1 promoterAvailable SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS305-mAID-LaM8
Plasmid#198415PurposeExpresesses mAID-Nb(LaM8) under the control of ADH1 promoter in budding yeastDepositorInsertmAID-Nb (LaM8)
ExpressionYeastPromoterADH1 promoterAvailable SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
MtPT4-eGFP
Plasmid#117095Purposeprotein expression in yeastDepositorInsertMtPT4-eGFP
TagsHisExpressionYeastPromoterAOXAvailable SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
PP93
Plasmid#78990PurposeSupplemental Figure 4. No Promoter.DepositorInsertGFP
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1TGt
Plasmid#44508DepositorInsertsUseSynthetic Biology; Expression regulator/reporterExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDS296
Plasmid#34985DepositorInsertePDZb1
ExpressionYeastPromoterGAL1Available SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
LD101
Plasmid#69160PurposeIntegration of GFP tag at Atg1 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg1-EGFP
TagsEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGK415-rtetTAK8N, L131L
Plasmid#165969PurposeExpresses rTetTA (K8N, L131L) mutantDepositorInserttetR
UseSynthetic BiologyTagsthree copies of VP16 (VP48)ExpressionYeastPromoterpPGK1Available SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K5
Plasmid#63210PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceFeb. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K4
Plasmid#63212PurposepCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceFeb. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS-Dhh1-PA (405)
Plasmid#121417PurposeExpressing Dhh1-PADepositorInsertDhh1promoter-Dhh1 ORF-PA-ADH1 terminator (DHH1 Budding Yeast)
ExpressionYeastAvailable SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPTK018-3b-RFP_Sec
Plasmid#84977PurposeType 3b, Red fluorescent proteinDepositorInsertRed fluorescent protein
UseSynthetic BiologyExpressionYeastMutationBsaI site removed (1677 c to g and 1794 a to g)PromoterNonAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCfB9337 (pgRNA_IV-1_NatMX)
Plasmid#161590PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IV-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
p42Hyg 3-F GW
Plasmid#58559PurposeMulticopy vector with Hygromycin resistance marker for triple Gateway insertionDepositorTypeEmpty backboneExpressionYeastAvailable SinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free GFP-NES
Plasmid#182491PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS
GFP-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10 and GAL7Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-Sec7-mScarlet2Ix6
Plasmid#140151PurposePlasmid for pop-in/pop-out replacement of Sec7 with Sec7-mScarlet2Ix6DepositorInsertSEC7
ExpressionYeastAvailable SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pILGFPU0 (R)
Plasmid#83562PurposeExpress yEGFP-ERG9 ER targeting peptide-CLN2PEST under the control of TEF1 promoterDepositorInsertTEF1 promoter-yEGFP-ERG9 ER targeting peptide-CLN2PEST
ExpressionYeastPromoterTEF1Available SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only