8,086 results

1. pJO818 (pRS425 Gal1-Ufd2-flag)

   Plasmid

   #159522

   Purpose

   expresses ufd2-flag in yeast cells

   Depositor

   Alexander Varshavsky

   Insert

   Ufd2
   

   Use

   Tags

   Flag

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. BB3aZ_14*

   Plasmid

   #98542

   Purpose

   empty BB3 for insertion and overexpression of 1 gene in P. pastoris

   Depositor

   Brigitte Gasser, Michael Sauer, Diethard Mattanovich

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRS316 GAL cluster

   Plasmid

   #61925

   Purpose

   Expression of GAL1 and GAL10 from S. cerevisiae cloned into pRS316

   Depositor

   Jon Houseley

   Article

   Cruz et al Elife. 2014 Feb 11;3:e01581. doi: 10.755

   Inserts

   GAL1 (GAL1 Budding Yeast)
   GAL10 (GAL10 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFTK019

   Plasmid

   #171291

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   PTET-10x-repeat promoter+CPgpdA (fused)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Promoter

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFTK017

   Plasmid

   #171289

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   PTET-5x-repeat promoter+CPgpdA (fused)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Promoter

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFTK038

   Plasmid

   #171310

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   hph, hygR, hygromycin selection marker
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Promoter

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFTK037

   Plasmid

   #171309

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   bleoR phleomycin, selection marker
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Promoter

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p416GAL1-Zipp.-L-IFP-F[1] (Yeast)

   Plasmid

   #52895

   Purpose

   Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   Zipp.-L-IFP-F[1]
   

   Use

   Tags

   L-IFP-F[1]

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pMEL14

   Plasmid

   #107920

   Purpose

   LEU2 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUAST BmOr2

    Plasmid

    #73926

    Purpose

    UAS induced expression of Bombyx mori odorant receptor 2 (OR2) cDNA

    Depositor

    Leslie Vosshall

    Article

    Nakagawa et al PLoS One. 2012;7(3):e32372. doi: 10.1371

    Insert

    BmOr2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAG413GPD-ccdB-EGFP

    Plasmid

    #14190

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMOD8A-iRGR-r11

    Plasmid

    #74369

    Purpose

    This plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.

    Depositor

    Eric Klavins

    Article

    Gander et al bioRxiv 041871

    Insert

    RGR-r11
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AHD1

    Available Since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFA6-mTFP1-His3MX6

    Plasmid

    #69850

    Purpose

    C-terminal tagging with mTFP1 using His3MX6 as a selection marker in S. cerevisiae.

    Depositor

    Liza Pon

    Article

    Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

    Insert

    mTFP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJW1510

    Plasmid

    #62364

    Purpose

    pFA6a pSEC63-BirA-mVenus-UBC6-TA::HIS5

    Depositor

    Jonathan Weissman

    Article

    Jan et al Science. 2014 Nov 7;346(6210):1257521. d

    Insert

    BirA-mVenus-Ubc6 tail anchor
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    L232H mutation compared to standard mVenus

    Promoter

    Sec63

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG415GAL-ccdB-EYFP

    Plasmid

    #14217

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGBW-m4046464

    Plasmid

    #148970

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pYC119

    Plasmid

    #63916

    Purpose

    Recipient overexpressing vector to construct FLAG tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 (Saccharomyces cerevisiae)

    Available Since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFA6a-link-mDsRed-SpHis5

    Plasmid

    #44639

    Purpose

    Yeast tagging vector to create gene fusion with mDsRed with SpHis5 selection

    Depositor

    Kurt Thorn

    Insert

    mDsRed
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYTK096

    Plasmid

    #65203

    Purpose

    Pre-Assembled Ura3 integration vector to be used in the Dueber YTK system.

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAG413GPD-ccdB-EYFP

    Plasmid

    #14214

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge