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pMTKM07

Plasmid

#233479

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and URA selection.

Depositor

Vincent Martin

Publication

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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T7pro-tRNA-ITSIle intron-T7tVar1-pRS426

Plasmid

#239294

Purpose

Expresses the tRNAIle intron bearing the 5' GGGAGA initially transcribed sequence (ITS); Plasmid #2 of the tet-on overexpression system

Depositor

Anita Hopper

Publication

Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

Inserts

T7 promoter
ITS-tRNAIle intron
T7 terminator - Var1

Expression

Yeast

Available since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pILFPQ13A7

Plasmid

#223837

Purpose

Expressing mScarletI3 under the control of the TEF1 promoter in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Publication

Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

Insert

pTEF1>mScarletI3>

Expression

Yeast

Promoter

TEF1

Available since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pKS1576

Plasmid

#239524

Purpose

Express Vba2p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur

Depositor

John Dueber

Insert

ScVba2p-mTurquoise2-2xCrk_SH3

Expression

Yeast

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLS410

Plasmid

#239123

Purpose

Minimal centromeric shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker KanMX

Depositor

Sahand Jamal Rahi

Publication

Scutteri et al Synth Biol (Oxf). 2025 May 21;10(1):ysaf

Type

Empty backbone

Use

Centromeric

Expression

Bacterial and Yeast

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRO227

Plasmid

#233835

Purpose

MoClo-YTK; ConL1/RE cassette; rtTA systems; tetO7-pLEU2m-mNeonGreen-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

tetO7-pLEU2m-mNeonGreen-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO236

Plasmid

#233847

Purpose

MoClo-YTK; ConL2/RE cassette; ABA systems; phlfO7-pLEU2m-mNeonGreen-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

phlfO7-pLEU2m-mNeonGreen-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN020

Plasmid

#233850

Purpose

MoClo-YTK; ConL2/RE cassette; Auxin systems; amtRO7-pLEU2m-mNeonGreen-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

amtRO7-pLEU2m-mNeonGreen-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN069

Plasmid

#233855

Purpose

MoClo-YTK; ConL2/RE cassette; NS3 systems; lexAO6-pLEU2m-mNeonGreen-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

lexAO6-pLEU2m-mNeonGreen-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAL004

Plasmid

#233863

Purpose

MoClo-YTK URA multigene plasmid with MCS; Tet systems; pRNR2-rtTA-v16-tADH1; tetO7-pLEU2m-MCS-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pRNR2-rtTA-v16-tADH1; tetO7-pLEU2m-MCS-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO174

Plasmid

#233809

Purpose

MoClo-YTK; type3A; ABA systems; Phlf-NES

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

Phlf-NES

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO117

Plasmid

#233823

Purpose

MoClo-YTK; type3; Reporter; mNeonGreen

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

mNeonGreen

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMY.PRC2

Plasmid

#234582

Purpose

Expression of ProtA-EZH2, SUZ12, EED and RBBP4

Depositor

Chen Davidovich

Publication

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

EZH2, SUZ12, EED, RBBP4 (EZH2, SUZ12, RBBP4, EED Human)

Expression

Yeast

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-Dendra2-Nat

Plasmid

#236494

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on Nat.

Depositor

Daniel Lew

Publication

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

Dendra2

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-sfGFP-G418

Plasmid

#236481

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on G418.

Depositor

Daniel Lew

Publication

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

sfGFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6-CFAPoptim-KAN

Plasmid

#221128

Purpose

Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::KANMX

Depositor

Allyson O’Donnell

Publication

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

FAPoptim

Expression

Yeast

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar1

Plasmid

#232738

Purpose

Expresses NADPH/NADP+ biosensor NAPstar1 in S. cerevisiae.

Depositor

Bruce Morgan

Publication

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar1

Expression

Yeast

Available since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar6

Plasmid

#232742

Purpose

Expresses NADPH/NADP+ biosensor NAPstar6 in S. cerevisiae.

Depositor

Bruce Morgan

Publication

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar6

Expression

Yeast

Available since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGBKT7_AvrSr62-5

Plasmid

#233532

Purpose

Expresses AvrSr62-5 in yeast

Depositor

Brande Wulff

Publication

Chen et al Science. 2025 Mar 28;387(6741):1402-1408

Insert

AvrSr62-5

Tags

GAL4 BD, Myc tag

Expression

Yeast

Promoter

ADH1

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGBKT7_AvrSr62-1

Plasmid

#233531

Purpose

Expresses AvrSr62-1 in yeast

Depositor

Brande Wulff

Publication

Chen et al Science. 2025 Mar 28;387(6741):1402-1408

Insert

AvrSr62-1

Tags

GAL4 BD, Myc tag

Expression

Yeast

Promoter

ADH1

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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