8,086 results

1. pJW1670

   Plasmid

   #112044

   Purpose

   CEN/ARS plasmid for GAL-inducible OLE1-V5-TEV-EGFP expression marked with URA

   Depositor

   Jonathan Weissman

   Article

   Costa et al Science. 2018 Jan 18. pii: science.aar36

   Insert

   OLE1
   

   Use

   Tags

   V5-TEV-EGFP

   Expression

   Yeast

   Mutation

   P319T

   Promoter

   GAL1

   Available Since

   May 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBW-m4046658

   Plasmid

   #149226

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp10

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp10 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pGBW-m4046682

   Plasmid

   #149197

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pGBW-m4046642

   Plasmid

   #149164

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp8

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp8 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pGBW-m4046687

   Plasmid

   #149148

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp7

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp7 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pAG424-ccdB

   Plasmid

   #70124

   Purpose

   Yeast Gateway TRP1 2micron vector, no promoter (GPD1 promoter removed)

   Depositor

   Jay Hesselberth

   Article

   Cherry et al RNA. 2018 Mar;24(3):313-323. doi: 10.126

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   None

   Available Since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EZ-L498

   Plasmid

   #131771

   Purpose

   PPGK1_FUSN_Citrine _PixE_TCYC1

   Depositor

   Jared Toettcher

   Article

   Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

   Insert

   PPGK1_FUSN_Citrine _PixE_TCYC1
   

   Use

   Integration into his3 locus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PGK

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPSG465

   Plasmid

   #158587

   Purpose

   Enzymes Bv-CYP76AD5, DODC, ARO4_K229L

   Depositor

   John Dueber

   Article

   Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

   Inserts

   Bv-CYP76AD5
   DODC
   ARO4_K229L
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K229L

   Promoter

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CHL1 wt-eGFP

   Plasmid

   #117876

   Purpose

   protein expression

   Depositor

   Geoffrey Chang

   Insert

   CHL1 wt-eGFP (AT5G40090 Mustard Weed)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ZmMATE2

    Plasmid

    #117094

    Purpose

    protein expression in yeast

    Depositor

    Geoffrey Chang

    Insert

    ZmMATE2
    

    Use

    Tags

    His

    Expression

    Yeast

    Mutation

    Promoter

    AOX

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. OsMATE

    Plasmid

    #117093

    Purpose

    protein expression in yeast

    Depositor

    Geoffrey Chang

    Insert

    AtTPT (ORF-HIS)
    

    Use

    Tags

    His

    Expression

    Yeast

    Mutation

    Promoter

    AOX

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AtFRD3

    Plasmid

    #117086

    Purpose

    protein expression in yeast

    Depositor

    Geoffrey Chang

    Insert

    AtFRD3
    

    Use

    Tags

    His

    Expression

    Yeast

    Mutation

    Promoter

    AOX

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAG426GAL-EYFP-ccdB

    Plasmid

    #14347

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LD229

    Plasmid

    #69192

    Purpose

    Integration of 2GFP tag at Atg29 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg29-2EGFP
    

    Use

    Tags

    2xEGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. plIVMD164

    Plasmid

    #160936

    Purpose

    In vivo mRNA display construct for GFP

    Depositor

    Saeed Tavazoie

    Article

    Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

    Insert

    MCP-sfGFP-HIS tag, 3'UTR MS2 Stem Loop
    

    Use

    Tags

    HIS tag, 3'UTR MS2 Stem Loop

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGBW-m4046268

    Plasmid

    #145423

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pGBW-m4046216

    Plasmid

    #145490

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pGBW-m4046286

    Plasmid

    #149147

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 surface glycoprotein (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pJT2

    Plasmid

    #98306

    Purpose

    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

    Insert

    PTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ERG20 N127W

    Promoter

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRG236

    Plasmid

    #64534

    Purpose

    Integrative E. coli/S. cerevisiae shuttle vector

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge